Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells

Bhat, Mahesh P.; Thendral, Venkatachalam; Uthappa, U.T.; Lee, Kyeong–Hwan; Kigga, Madhuprasad; Altalhi, Tariq; Kurkuri, Mahaveer D.; Kant, Kamal

doi:10.3390/bios12040220

Cited by 26 publications

(18 citation statements)

References 178 publications

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“…Because of the scarcity of CTCs, isolating enough CTCs is a crucial step for single-cell sequencing, which relies on the efficiency of enrichment and isolation of the system. With the advancement of microfluidic instruments in recent years, the enrichment and capture efficiency of most technologies can reach 80–90% or higher [ 12 ]. The CTC platforms based on microfluidic technology have better capture efficiency than other technologies.…”

Section: Discussionmentioning

confidence: 99%

Isolation of TTF-1 Positive Circulating Tumor Cells for Single-Cell Sequencing by Using an Automatic Platform Based on Microfluidic Devices

Jou

Ho²,

Huang³

et al. 2022

IJMS

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Single-cell sequencing provides promising information in tumor evolution and heterogeneity. Even with the recent advances in circulating tumor cell (CTC) technologies, it remains a big challenge to precisely and effectively isolate CTCs for downstream analysis. The Cell RevealTM system integrates an automatic CTC enrichment and staining machine, an AI-assisted automatic CTC scanning and identification system, and an automatic cell picking machine for CTC isolation. H1975 cell line was used for the spiking test. The identification of CTCs and the isolation of target CTCs for genetic sequencing were performed from the peripheral blood of three cancer patients, including two with lung cancer and one with both lung cancer and thyroid cancer. The spiking test revealed a mean recovery rate of 81.81% even with extremely low spiking cell counts with a linear relationship between the spiked cell counts and the recovered cell counts (Y = 0.7241 × X + 19.76, R2 = 0.9984). The three cancer patients had significantly higher TTF-1+ CTCs than healthy volunteers. All target CTCs were successfully isolated by the Cell Picker machine for a subsequent genetic analysis. Six tumor-associated mutations in four genes were detected. The present study reveals the Cell RevealTM platform can precisely identify and isolate target CTCs and then successfully perform single-cell sequencing by using commercially available genetic devices.

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Section: Discussionmentioning

confidence: 99%

Isolation of TTF-1 Positive Circulating Tumor Cells for Single-Cell Sequencing by Using an Automatic Platform Based on Microfluidic Devices

Jou

Ho²,

Huang³

et al. 2022

IJMS

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“…In the past decade, microfluidic technology has developed more rapidly because of its many advantages, such as high throughput, low cost, high efficiency, precision and low sample consumption [ 30 ]. For example, Wang et al, have reported a capture efficiency about 81% and a high purity of 83% by using multifunctional microsphere-assisted inertial microfluidics to isolate fnRBCs [ 31 ].…”

Section: Microfluidics Technologies For Nipdmentioning

confidence: 99%

“…Due to advances in fabrication technique of microfluidic system, recovery rates of CRCs can reach 80–90% or higher for most systems [ 30 ]. Chromosomal aberrations can be detected using on-chip FISH.…”

Section: Isolation Of Fetal Cellsmentioning

confidence: 99%

Recent Advances of Microfluidic Platform for Cell Based Non-Invasive Prenatal Diagnosis

Jou

Lo²,

Ling³

2023

IJMS

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The purpose of the present review is to try to highlight recent advances in the application of microfluidic technology on non-invasive prenatal diagnosis (NIPD). The immunoaffinity based microfluidic technology is the most common approach for NIPD, followed by size-based microfluidic methods. Immunoaffinity microfluidic methods can enrich and isolate circulating fetal extravillous trophoblasts (fEVTs) or fetal nucleated red blood cells (fnRBCs) for NIPD by using specific antibodies, but size-based microfluidic systems are only applied to isolate fEVTs. Most studies based on the immunoaffinity microfluidic system gave good results. Enough fetal cells were obtained for chromosomal and/or genetic analysis in all blood samples. However, the results from studies using size-based microfluidic systems for NIPD are less than ideal. In conclusion, recent advances in microfluidic devices make the immunoaffinity based microfluidic system potentially a powerful tool for cell-based NIPD. However, more clinical validation is needed.

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“…In recent years, microfluidic devices have been increasingly utilized for different biophysical tasks, such as cell cultivation [ 1 , 2 , 3 , 4 , 5 ], cell separation [ 6 , 7 , 8 , 9 , 10 , 11 ], expansion of CTC from patient blood [ 12 , 13 , 14 , 15 , 16 , 17 ], flow cytometric analysis and cell manipulation [ 18 , 19 , 20 , 21 , 22 , 23 ], tissue engineering (Organ-On-a-Chip) [ 24 , 25 , 26 , 27 , 28 , 29 ], cell sorting and cell counting [ 30 , 31 , 32 , 33 ], and point of care (POC) diagnosis [ 34 , 35 , 36 , 37 , 38 , 39 ]. Many biochemical analyses can be screened at a faster rate in disease diagnosis [ 40 , 41 ].…”

Section: Introductionmentioning

confidence: 99%

Novel Pumping Methods for Microfluidic Devices: A Comprehensive Review

2022

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This review is an account of methods that use various strategies to control microfluidic flow control with high accuracy. The reviewed systems are divided into two large groups based on the way they create flow: passive systems (non-mechanical systems) and active (mechanical) systems. Each group is presented by a number of device fabrications. We try to explain the main principles of operation, and we list advantages and disadvantages of the presented systems. Mechanical systems are considered in more detail, as they are currently an area of increased interest due to their unique precision flow control and “multitasking”. These systems are often applied as mini-laboratories, working autonomously without any additional operations, provided by humans, which is very important under complicated conditions. We also reviewed the integration of autonomous microfluidic systems with a smartphone or single-board computer when all data are retrieved and processed without using a personal computer. In addition, we discuss future trends and possible solutions for further development of this area of technology.

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Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells

Cited by 26 publications

References 178 publications

Isolation of TTF-1 Positive Circulating Tumor Cells for Single-Cell Sequencing by Using an Automatic Platform Based on Microfluidic Devices

Isolation of TTF-1 Positive Circulating Tumor Cells for Single-Cell Sequencing by Using an Automatic Platform Based on Microfluidic Devices

Recent Advances of Microfluidic Platform for Cell Based Non-Invasive Prenatal Diagnosis

Novel Pumping Methods for Microfluidic Devices: A Comprehensive Review

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