Label-free detection with micro optical fluidic systems (MOFS): a review

Liu, A. Q.; Huang, Hung Ji; Chin, Lip Ket; Yu, Yi; Li, X. C.

doi:10.1007/s00216-008-1878-2

Cited by 68 publications

(52 citation statements)

References 88 publications

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“…There has already been a considerable amount of insightful review articles on related topics. [67][68][69][70][71][72] Various detection methods exist in the field of chemical, biological diagnosis, or analysis on microfluidic platforms.…”

Section: Detection Methodsmentioning

confidence: 99%

Microfluidic sensing: state of the art fabrication and detection techniques

2011

J. Biomed. Opt.

175

141

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Section: Detection Methodsmentioning

confidence: 99%

Microfluidic sensing: state of the art fabrication and detection techniques

2011

J. Biomed. Opt.

175

141

View full text Add to dashboard Cite

“…[5][6][7] The great success of microfluidics in many applications such as chemical analysis, biomedical assay, and drug screening has well demonstrated its remarkable capabilities in dealing with particles, fluids, and light in an integrated platform. [7][8][9][10][11][12][13][14][15][16] Thanks to its merits of flexible flow control, large surface-area-to-volume ratio, and compact size, the microfluidics could benefit the photocatalysis [16][17][18][19][20] in various aspects such as fast mass a) E-mail: apzhang@polyu.edu.hk. Tel.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic reactors for visible-light photocatalytic water purification assisted with thermolysis

Wang¹,

Tan²,

Wan³

et al. 2014

Biomicrofluidics

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Photocatalytic water purification using visible light is under intense research in the hope to use sunlight efficiently, but the conventional bulk reactors are slow and complicated. This paper presents an integrated microfluidic planar reactor for visible-light photocatalysis with the merits of fine flow control, short reaction time, small sample volume, and long photocatalyst durability. One additional feature is that it enables one to use both the light and the heat energy of the light source simultaneously. The reactor consists of a BiVO 4 -coated glass as the substrate, a blank glass slide as the cover, and a UV-curable adhesive layer as the spacer and sealant. A blue light emitting diode panel (footprint 10 mm Â 10 mm) is mounted on the microreactor to provide uniform irradiation over the whole reactor chamber, ensuring optimal utilization of the photons and easy adjustments of the light intensity and the reaction temperature. This microreactor may provide a versatile platform for studying the photocatalysis under combined conditions such as different temperatures, different light intensities, and different flow rates. Moreover, the microreactor demonstrates significant photodegradation with a reaction time of about 10 s, much shorter than typically a few hours using the bulk reactors, showing its potential as a rapid kit for characterization of photocatalyst performance.

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“…18 Confocal microscopy requires fluorescent labeling for detection, which is time-consuming, complex, and may not always be suitable for rapid biophysical and routine characterization tasks. 22 In addition, real-time hybridization monitoring using confocal microscopy cannot be performed, because it is hard to distinguish between unbound targets in solution and those that have been hybridized to the probe on the surface, as both will fluoresce when imaged. 23 Photonic microcavities such as circular resonators or spherical resonators are promising optical label-free detection setups.…”

Section: Introductionmentioning

confidence: 99%

Photonic detection and characterization of DNA using sapphire microspheres

et al. 2014

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Abstract. A microcavity-based deoxyribonucleic acid (DNA) optical biosensor is demonstrated for the first time using synthetic sapphire for the optical cavity. Transmitted and elastic scattering intensity at 1510 nm are analyzed from a sapphire microsphere (radius 500 μm, refractive index 1.77) on an optical fiber half coupler. The 0.43 nm angular mode spacing of the resonances correlates well with the optical size of the sapphire sphere. Probe DNA consisting of a 36-mer fragment was covalently immobilized on a sapphire microsphere and hybridized with a 29-mer target DNA. Whispering gallery modes (WGMs) were monitored before the sapphire was functionalized with DNA and after it was functionalized with single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). The shift in WGMs from the surface modification with DNA was measured and correlated well with the estimated thickness of the add-on DNA layer. It is shown that ssDNA is more uniformly oriented on the sapphire surface than dsDNA. In addition, it is shown that functionalization of the sapphire spherical surface with DNA does not affect the quality factor (Q ≈ 10 4 ) of the sapphire microspheres. The use of sapphire is especially interesting because this material is chemically resilient, biocompatible, and widely used for medical implants.

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Label-free detection with micro optical fluidic systems (MOFS): a review

Cited by 68 publications

References 88 publications

Microfluidic sensing: state of the art fabrication and detection techniques

Microfluidic sensing: state of the art fabrication and detection techniques

Microfluidic reactors for visible-light photocatalytic water purification assisted with thermolysis

Photonic detection and characterization of DNA using sapphire microspheres

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