Label-free detection of Babesia bovis infected red blood cells using impedance spectroscopy on a microfabricated flow cytometer

Küttel, Claudia; Nascimento, Elisabete; Demierre, Nicolas; Silva, Tiago; Braschler, Thomas; Renaud, Philippe; Oliva, Abel

doi:10.1016/j.actatropica.2007.03.002

Cited by 57 publications

(37 citation statements)

References 24 publications

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“…The coplanar electrodes design (Fig. 9a) proposed by Gawad et al (2001) was adapted for single particle/cell impedance sensing and counting by many authors (Nieuwenhuis et al 2004;Wood et al 2005Wood et al , 2007aMorgan et al 2006;Benazzi et al 2007;Küttel et al 2007;Iliescu et al 2007;Rodriguez-Trujillo et al 2007, 2008Murali et al 2009). High-speed measurements were demonstrated by Wood et al (2005) who used a radio frequency resonance detection technique with coplanar electrodes to eliminate stray capacitance effect at high frequencies and particles could be counted at a throughput of 25000/s.…”

Section: Microfluidic Impedance Cytometrymentioning

confidence: 99%

Single-cell microfluidic impedance cytometry: a review

Sun

Morgan

2010

Microfluid Nanofluid

491

391

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Lab-on-chip technologies are being developed for multiplexed single cell assays. Impedance offers a simple non-invasive method for counting, identifying and monitoring cellular function. A number of different microfluidic devices for single cell impedance have been developed. These have potential applications ranging from simple cell counting and label-free identification of different cell types or detecting changes in cell morphology after invasion by parasites. Devices have also been developed that trap single cells and continuously record impedance data. This technology has applications in basic research, diagnostics, or non-invasively probing cell function at the single-cell level. This review will describe the underlying principles of impedance analysis of particles. It then describes the state-of-the-art in the field of microfluidic impedance flow cytometry. Finally, future directions and challenges are discussed.

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Section: Microfluidic Impedance Cytometrymentioning

confidence: 99%

Single-cell microfluidic impedance cytometry: a review

Sun

Morgan

2010

Microfluid Nanofluid

491

391

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“…A voltage is applied at several different frequencies and the change in current is analysed to determine the cell dielectric properties. Microfluidic impedance cytometry has been used to analyse micro-organisms [5][6][7] , erythrocytes 8,9 , leukocytes 10,11 , platelets 12,13 , and animal and human cell lines [14][15][16] . Low frequency (e.g.…”

Section: Introductionmentioning

confidence: 99%

High accuracy particle analysis using sheathless microfluidic impedance cytometry

et al. 2016

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This paper describes a new design of microfluidic impedance cytometer enabling accurate characterization of particles without the need for focusing. The approach uses multiple pairs of electrodes to measure the transit time of particles through the device using two simultaneous different current measurements, a transverse (top to bottom) current and an oblique current. This gives a new metric that can be used to estimate the vertical position of the particle trajectory through the microchannel. This parameter effectively compensates for the non-uniform electric field in the channel that is an unavoidable consequence of the use of planar parallel facing electrodes. The new technique is explained and validated using numerical modelling. Impedance data for 5, 6 and 7 µm particles are collected and compared with simulations. The method gives excellent Coefficient of Variation in (electrical) radius of particles of 1% for a sheath less configuration.

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“…14,15 Impedance cytometry can be used to differentiate many different cell types, for example, the three main populations of human leukocytes, 16,17 stem cells, 18 or parasite infected cells. 19 Recently, Choi et al 20 developed a DC microfluidic impedance cytometer which can identify cancer cells directly in whole blood without dilution. Using samples spiked with ovarian cancer cells, the detection efficiency was 88%.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic impedance cytometry of tumour cells in blood

2014

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The dielectric properties of tumour cells are known to differ from normal blood cells, and this difference can be exploited for label-free separation of cells. Conventional measurement techniques are slow and cannot identify rare circulating tumour cells (CTCs) in a realistic timeframe. We use high throughput single cell microfluidic impedance cytometry to measure the dielectric properties of the MCF7 tumour cell line (representative of CTCs), both as pure populations and mixed with whole blood. The data show that the MCF7 cells have a large membrane capacitance and size, enabling clear discrimination from all other leukocytes. Impedance analysis is used to follow changes in cell viability when cells are kept in suspension, a process which can be understood from modelling time-dependent changes in the dielectric properties (predominantly membrane conductivity) of the cells. Impedance cytometry is used to enumerate low numbers of MCF7 cells spiked into whole blood. Chemical lysis is commonly used to remove the abundant erythrocytes, and it is shown that this process does not alter the MCF7 cell count or change their dielectric properties. Combining impedance cytometry with magnetic bead based antibody enrichment enables MCF7 cells to be detected down to 100 MCF7 cells in 1 ml whole blood, a log 3.5 enrichment and a mean recovery of 92%. Microfluidic impedance cytometry could be easily integrated within complex cell separation systems for identification and enumeration of specific cell types, providing a fast in-line single cell characterisation method.

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Label-free detection of Babesia bovis infected red blood cells using impedance spectroscopy on a microfabricated flow cytometer

Cited by 57 publications

References 24 publications

Single-cell microfluidic impedance cytometry: a review

Single-cell microfluidic impedance cytometry: a review

High accuracy particle analysis using sheathless microfluidic impedance cytometry

Microfluidic impedance cytometry of tumour cells in blood

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