Single-cell microfluidic impedance cytometry: a review

Sun, Tao; Morgan, Hywel

doi:10.1007/s10404-010-0580-9

Cited by 491 publications

(420 citation statements)

References 121 publications

(130 reference statements)

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“…This opens the route to a possible integration of a Coulter counter unit on chip. Coulter counters integrated with micro fluid systems have been reported previously (18)(19)(20)(21) and on-going work in our group now targets the development of a microfluidic system with integrated acoustophoretic and Coulter counter functionality.…”

Section: Resultsmentioning

confidence: 99%

Label‐free somatic cell cytometry in raw milk using acoustophoresis

Grenvall

Folkenberg²,

Augustsson

et al. 2012

Cytometry Pt A

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A microfluidic system for cell enumeration in raw milk was developed. The new method, preconditions the milk sample using acoustophoresis that removes lipid particles which are larger than a few micrometers. The acoustophoretic preprocessing eliminates the need for conventional sample preparation techniques, which include chemical solvents, cell labeling and centrifugation, and facilitates rapid cell enumeration using microscopy or coulter counter measurements. By introducing an acoustic standing wave with three pressure nodes in a microchannel at the same time as the milk sample is laminated to the channel center, lipids are acoustically driven to the closest pressure antinode at each side of the channel center and the cells in the milk sample are focused in the central pressure node. The extracted center fraction with cells becomes sufficiently clean from lipid vesicles to enable enumeration of somatic cells without any labeling step either by direct light microscopy or by coulter counting. Obtained lipid free milk fractions clearly revealed the cell fraction when analyzed by Coulter Counting.

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Section: Resultsmentioning

confidence: 99%

Label‐free somatic cell cytometry in raw milk using acoustophoresis

Grenvall

Folkenberg²,

Augustsson

et al. 2012

Cytometry Pt A

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“…[109][110][111] More details on DEP and ROT models can be found in other reviews. 9,112 The most popular ROT setup uses four electrodes connected to sine waves in phase quadrature. 91,113 Cells are placed in the center of the four electrodes (e.g., using laser tweezers).…”

Section: Electrorotationmentioning

confidence: 99%

“…130 More details on impedance flow cytometry can be found elsewhere. 112,130 In addition to coplanar and parallel facing electrode designs, Mernier et al demonstrated ''liquid electrodes'' to discriminate living and dead yeast cells (see Fig. 4(c)).…”

Section: Impedance Flow Cytometry (Ifc)mentioning

confidence: 99%

Recent advances in microfluidic techniques for single-cell biophysical characterization

et al. 2013

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“…DEP, integrated within microfluidic systems, is more applicable to the manipulation and separation of single cells than their analysis [19,20]. In contrast, EIS enables the frequency-dependent multiparameter readout of cellular and even subcellular information in a high-throughput setting [21,22] or dynamically [23]. EIS provides information related to the cell size at lower frequencies (from hundreds of kHz to MHz), related to the cell membrane capacitance at higher frequencies (several MHz), and information on intracellular features at even higher frequencies.…”

Section: Introductionmentioning

confidence: 99%

“…Microfluidic devices with integrated EIS functions have been developed already for singlecell analysis in the past decade [13,14,21]. Most of those microfluidic devices, called electrical impedance cytometers, were used to characterize suspended biological samples in a flow-through setup.…”

Section: Introductionmentioning

confidence: 99%

Real-time monitoring of immobilized single yeast cells through multifrequency electrical impedance spectroscopy

et al. 2014

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We present a microfluidic device, which enables single cells to be reliably trapped and cultivated while simultaneously being monitored by means of multifrequency electrical impedance spectroscopy (EIS) in the frequency range of 10 kHz-10 MHz. Polystyrene beads were employed to characterize the EIS performance inside the microfluidic device. The results demonstrate that EIS yields a low coefficient of variation in measuring the diameters of captured beads (~0.13 %). Budding yeast, Saccharomyces cerevisiae, was afterwards used as model organism. Single yeast cells were immobilized and measured by means of EIS. The bud growth was monitored through EIS at a temporal resolution of 1 min. The size increment of the bud, which is difficult to determine optically within a short time period, can be clearly detected through EIS signals. The impedance measurements also reflect the changes in position or motion of single yeast cells in the trap. By analyzing the multifrequency EIS data, cell motion could be qualitatively discerned from bud growth. The results demonstrate that single-cell EIS can be used to monitor cell growth, while also detecting potential cell motion in real-time and label-free approach, and that EIS constitutes a sensitive tool for dynamic single-cell analysis.

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Single-cell microfluidic impedance cytometry: a review

Cited by 491 publications

References 121 publications

Label‐free somatic cell cytometry in raw milk using acoustophoresis

Label‐free somatic cell cytometry in raw milk using acoustophoresis

Recent advances in microfluidic techniques for single-cell biophysical characterization

Real-time monitoring of immobilized single yeast cells through multifrequency electrical impedance spectroscopy

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