Label-free bifunctional electrochemiluminescence aptasensor for detection of adenosine and lysozyme

Wang, Haiyan; Gong, Wei; Tan, Zhijun; Yin, Xunxun; Wang, Lun

doi:10.1016/j.electacta.2012.05.056

Cited by 31 publications

(21 citation statements)

References 22 publications

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“…Changes in lysozyme amounts are related to some health problems, for example the increasing concentration of lysozyme in serum and urine may be caused by leukemia and several kidney problems . Many aptamer‐based methods for the detection of lysozyme have been developed in recent years, including fluorescence , electrochemiluminescence , surface plasmon resonance scattering . Among these methods, fluorescence detection has the advantages of high sensitivity, rapidity and simplicity.…”

Section: Introductionmentioning

confidence: 99%

Detection of lysozyme with aptasensor based on fluorescence resonance energy transfer from carbon dots to graphene oxide

Hou

Wang

et al. 2016

Luminescence

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A photoluminescent aptasensor has been developed for the detection of lysozyme based on fluorescence resonance energy transfer (FRET) between the carbon dots (CDs) and graphene oxide (GO). In the sensing system, the CDs-labeled aptamer is adsorbed onto the GO surface and the photoluminescence (PL) signal of the CDs is effectively quenched by GO. Addition of lysozyme can cause a significant FRET inhibition and recover the PL signal of the CDs due to the specific combination of lysozyme and its aptamer and the removal of the CDs-labeled aptamer from GO surface. Under optimal conditions, the ratio of PL intensity change at 440 nm of the sensing system before and after the addition of lysozyme shows a good linear relationship against the concentration of lysozyme in the range of 0.01-2 μg/mL, with a low detection limit of 1 × 10 À3 μg/mL. In addition, the aptasensor has good selectivity so it can distinguish lysozyme with no or little interference by many other biomolecules. It was applied to the detection of lysozyme in human sera with satisfactory recoveries. The results demonstrate the applicability of the aptasensor for monitoring lysozyme in real samples.

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Section: Introductionmentioning

confidence: 99%

Detection of lysozyme with aptasensor based on fluorescence resonance energy transfer from carbon dots to graphene oxide

Hou

Wang

et al. 2016

Luminescence

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“…In general, the results demonstrated that the proposed ECL aptasensor could be potentially used for the proteins detection in a complex biological environment. Furthermore, we have summarized the performances of several aptamer-based sensing platforms [34,57,[63][64][65][66][67][68] in comparison to this developed method (Table S1 and Table S2) and the results indicate that our method has comparable or even lower detection limits, demonstrating its high sensitivity without any further labelling or amplification process.…”

Section: Application To the Real Sample Of The Ecl Aptasensormentioning

confidence: 93%

Ultrasensitive and Signal‐on Electrochemiluminescence Aptasensor Using the Multi‐tris(bipyridine)ruthenium(II)‐β‐cyclodextrin Complexes

Zhao

Chen

et al. 2014

Chin. J. Chem.

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An ultrasensitive and signal-on electrochemiluminescence (ECL) aptasensor to detect target protein (thrombin or lysozyme) was developed using the host-guest recognition between a metallocyclodextrin complex and single-stranded DNA (ss-DNA). The aptasensor uses both the photoactive properties of the metallocyclodextrins named multi-tris(bipyridine)ruthenium(II)-β-cyclodextrin complexes and their specific recognition with ss-DNA, which amplified the ECL signal without luminophore labeling. After investigating the ECL performance of different multi-tris(bipyridine)ruthenium(II)-β-cyclodextrin (multi-Ru-β-CD) complexes, tris-tris(bipyridine)-ruthenium(II)-β-cyclodextrin (tris(bpyRu)-β-CD) was selected as a suitable host molecule to construct an atasensor. First, double-stranded DNA (ds-DNA) formed by hybridization of the aptamer and its target DNA was attached to a glassy carbon electrode via coupling interaction, which showed low ECL intensity with 2-(dibutylamino) ethanol (DBAE) as coreactant, because of the weak recognition between ds-DNA and tris(bpyRu)-β-CD. Upon addition of the corresponding protein, the ECL intensity increased when target ss-DNA was released because of the higher stability of the aptamer-protein complex than the aptamer-DNA one. A linear relationship was observed in the range of 0.01 pmol/L to 100 pmol/L between ECL intensity and the logarithm of thrombin concentrations with a limited detection of 8.5 fmol/L (S/N＝3). Meanwhile, the measured concentration of lysozyme was from 0.05 pmol/L to 500 pmol/L and the detection limit was 33 fmol/L (S/N＝3). The investigations of proteins in human serum samples were also performed to demonstrate the validity of detection in real clinical samples. The simplicity, high sensitivity and specificity of this aptasensor show great promise for practical applications in protein monitoring and disease diagnosis.

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“…The development of label‐free ECL aptasensors could overcome these drawbacks . Wang et al used Ru(phen) 3 2+ as the probe to develop an aptasensor for adenosine and lysozyme, with the linear ranges of 0.5 × 10 −9 to 7 × 10 −9 mol/L and 0.5 to 9 ng/mL, respectively. As indicated in Figure , Liu et al prepared the ordered mesoporous silica films (MSFs) by in situ growth on an ITO electrode .…”

Section: The Application Of Ecl Fna‐based Sensors In Food Contaminantsmentioning

confidence: 99%

Electrochemiluminescent functional nucleic acids‐based sensors for food analysis

Zhu

Wang

et al. 2019

Luminescence

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Foodborne contaminants widely exist in foods, which can lead to various foodborne diseases and food safety issues. The development of quick, sensitive and universal analytical approaches for foodborne contaminants is imperative. Electrochemiluminescent functional nucleic acids (ECL FNAs)‐based sensors are a series of sensing devices using FNAs as the recognition elements and ECL as the transducer. Contributing to the specific recognition ability of FNA and the high sensitivity of ECL, ECL FNA‐based sensors are considered to be of great application potential for foodborne contaminants monitoring. This review mainly presents the applications of ECL FNA‐based sensors for foodborne contaminants (including microorganisms, mycotoxins, allergens, antibiotics, heavy metal ions, pesticides and some illegal additives). In general, the application of ECL FNA‐based sensors in the field of food analysis is just in its infancy. Although there are several limitations and challenges, it is envisaged that ECL FNA‐based sensors will have broad prospects for food analysis in the future.

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Label-free bifunctional electrochemiluminescence aptasensor for detection of adenosine and lysozyme

Cited by 31 publications

References 22 publications

Detection of lysozyme with aptasensor based on fluorescence resonance energy transfer from carbon dots to graphene oxide

Detection of lysozyme with aptasensor based on fluorescence resonance energy transfer from carbon dots to graphene oxide

Ultrasensitive and Signal‐on Electrochemiluminescence Aptasensor Using the Multi‐tris(bipyridine)ruthenium(II)‐β‐cyclodextrin Complexes

Electrochemiluminescent functional nucleic acids‐based sensors for food analysis

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