Detection of lysozyme with aptasensor based on fluorescence resonance energy transfer from carbon dots to graphene oxide

Wu, Jing; Hou, Yu; Wang, Peiyao; Wang, Zhenni; Li, Yanjie; Wang, Shan; Yang, Mei

doi:10.1002/bio.3092

Cited by 27 publications

(15 citation statements)

References 28 publications

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“…Earlier, electron acceptance ability of graphene has been utilized in sensing of potassium ion in presence of crown ether via fluorescence resonance energy transfer in CDs‐graphene . In another study, higher electronic conductivity and large specific surface area of GO were used to detect lysozyme protein based on fluorescence energy transfer from CDs to GO . In this regard, large specific surface area, electron acceptance ability and higher electronic conductivity of graphitic materials (GMs) could play a key role in the optical sensing.…”

Section: Introductionmentioning

confidence: 99%

Green Synthesis of Carbon Dot Weak Gel from Pear Juice: Optical Properties and Sensing Application

Mondal

Srivastava

2018

ChemistrySelect

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Herein, we report for the first time a low-cost and eco-friendly carbon dot weak gel (CDWG) in the absence of any gelling agent from a pear juice as a green precursor. Our findings showed that the volatile nature of acetone, basic media, and cellulose content in pear juice played key roles in the formation of CDWG. This fabrication strategy of CDWG from a green precursor in absence of gelling agent could be more advantageous over CD-hybrids gel. Further, the fabricated gel exhibited excitation dependent red shifted multicolor (blue-green-yellow-orange) emission due to the presence of characteristic hydrophilic surface in CDs. The other unique feature of the work involved utilization of inherent fluorescent property of CDs in establishing optical interaction between 0D CDWG and 2D reduced graphene oxide (G) and graphene oxide (GO). It may be concluded that the quenching is mainly guided by the electron rich CDs and electron acceptance ability, large specific surface area of G and GO. It is also interesting to note that the introducing of gel property in CDs exhibit improved sensing efficiency and limit of detection of CDWG (1.5 μg ml À 1 ) for G. Further, such optical sensing could be beneficial for the fabrication of optoelectronics, photovoltaic devices and sensing of bio-molecules and metal ions.[a] J.

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Section: Introductionmentioning

confidence: 99%

Green Synthesis of Carbon Dot Weak Gel from Pear Juice: Optical Properties and Sensing Application

Mondal

Srivastava

2018

ChemistrySelect

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“…

We report on metal-non-metal doped carbon dots with very high photoluminescent properties in solution. [5][6][7][8] Typically displaying size and excitation dependent luminescence properties, Cdots have received great attention in the present scenario as nascent nano-dots, especially in the field where size, cost of the material and biocompatibility are critical. Our aim is to develop such dual doped carbon dots which can also serve living cell imaging with easy permeation towards cells and show non-cytotoxic attributes.

…”

mentioning

confidence: 99%

Dual doped biocompatible multicolor luminescent carbon dots for bio labeling, UV‐active marker and fluorescent polymer composite

et al. 2018

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We report on metal-non-metal doped carbon dots with very high photoluminescent properties in solution. Magnesium doping to tamarind extract associated with nitrogen-doping is for the first time reported here which also produce very high quantum yield. Our aim is to develop such dual doped carbon dots which can also serve living cell imaging with easy permeation towards cells and show non-cytotoxic attributes. More importantly, the chemical signatures of the carbon dots unveiled in this work can support their easy solubilization into water; even in sub-ambient temperature. The cytotoxicity assay proves the almost negligible cytotoxic effect against human cell lines. Moreover, the use of carbon dots in UV-active marker and polymer composites are also performed which gave clear distinguishable features of fluorescent nanoparticles. Hitherto, the carbon dots can be commercially prepared without adopting any rigorous methods and also can be used as non-photo-bleachable biomarkers of living cells.

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“…Sensitive and selective detection of biomolecules in biomedical technologies is highly crucial in biomedical research and clinical diagnosis. Biomolecule determination methods are based on immunoreactions, which are rapid and sensitive methods . In this study, we have originated a detection method for lysozyme through SPR sensor for the potential diagnosis application in leukemia, meningitis, and other kidney problems without any labelling process require that means can be saved time and cost.…”

Section: Resultsmentioning

confidence: 99%

“…Biomolecule determination methods are based on immunoreactions, which are rapid and sensitive methods. 29 In this study, we have originated a detection method for lysozyme through SPR sensor for the potential diagnosis application in leukemia, meningitis, and other kidney problems without any labelling process require that means can be saved time and cost. The SPR sensor was prepared by modification of the gold surface with the hydrophobic PMAPA nanoparticles and then characterized by ATR-FTIR, AFM, and WCA measurements.…”

Section: Discussionmentioning

confidence: 99%

Synthesis of hydrophobic nanoparticles for real‐time lysozyme detection using surface plasmon resonance sensor

Saylan

Yılmaz

Derazshamshir

et al. 2017

J of Molecular Recognition

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Diagnostic biomarkers such as proteins and enzymes are generally hard to detect because of the low abundance in biological fluids. To solve this problem, the advantages of surface plasmon resonance (SPR) and nanomaterial technologies have been combined. The SPR sensors are easy to prepare, no requirement of labelling and can be detected in real time. In addition, they have high specificity and sensitivity with low cost. The nanomaterials have also crucial functions such as efficiency improvement, selectivity, and sensitivity of the detection systems. In this report, an SPR-based sensor is developed to detect lysozyme with hydrophobic poly (N-methacryloyl-(L)-phenylalanine) (PMAPA) nanoparticles. The SPR sensor was first characterized by attenuated total reflection-Fourier transform infrared, atomic force microscope, and water contact angle measurements and performed with aqueous lysozyme solutions. Various concentrations of lysozyme solution were used to calculate kinetic and affinity coefficients. The equilibrium and adsorption isotherm models of interactions between lysozyme solutions and SPR sensor were determined and the maximum reflection, association, and dissociation constants were calculated by Langmuir model as 4.87, 0.019 nM , and 54 nM, respectively. The selectivity studies of SPR sensor were investigated with competitive agents, hemoglobin, and myoglobin. Also, the SPR sensor was used four times in adsorption/desorption/recovery cycles and results showed that, the combination of optical SPR sensor with hydrophobic ionizable PMAPA nanoparticles in one mode enabled the detection of lysozyme molecule with high accuracy, good sensivity, real-time, label-free, and a low-detection limit of 0.66 nM from lysozyme solutions. Lysozyme detection in a real sample was performed by using chicken egg white to evaluate interfering molecules present in the medium.

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Detection of lysozyme with aptasensor based on fluorescence resonance energy transfer from carbon dots to graphene oxide

Cited by 27 publications

References 28 publications

Green Synthesis of Carbon Dot Weak Gel from Pear Juice: Optical Properties and Sensing Application

Green Synthesis of Carbon Dot Weak Gel from Pear Juice: Optical Properties and Sensing Application

Dual doped biocompatible multicolor luminescent carbon dots for bio labeling, UV‐active marker and fluorescent polymer composite

Synthesis of hydrophobic nanoparticles for real‐time lysozyme detection using surface plasmon resonance sensor

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