L3T4-Positive cells in the lymph nodes during induction phase of contact hypersensitivity reaction of mice: flow cytometric analysis

Flow Cytometric Analysis of Protein Kinase C in Lymph Node Cells during the Induction Phase of Contact Hypersensitivity Reaction

“…It is known that Con A and PHA, both vegetable lectins, promote DNA synthesis of T cells in vitro. In vivo, on the other hand, these vegetable lectin antigens activate lymphocytes through the cell membrane receptors, as previously reported by us (6). In other words, the behavior of the lymph node cells of PCI-A/O mice in our experimental system prove that cells with a positive IL-2 receptor reaction are increased by priming.…”

Phenotypic Analysis of Lymphocyte Subpopulations in Lymph Nodes Draining the Ear Following Exposure to Contact Allergens and Irritants

“…There exist few reports on the phenotypic characterization of the DLN during the induction phase of a contact sensitization response. A study by Morita et al (1987) demonstrated that T cells were preferentially accumulating in lymph nodes draining the abdomen because L3T4 cells in BALB/c mice increased from 51.7 to 79.3% on the 5th day after a single application of DNFB in acetone:olive oil. This is in contrast to studies presented here, though we did not evaluate DNFB or abdominal lymph nodes.…”