Flow Cytometric Analysis of Protein Kinase C in Lymph Node Cells during the Induction Phase of Contact Hypersensitivity Reaction

Morita, Hideki; Yamagata, M; Inohara, Shinichi; Sagami, Seichiro

doi:10.1111/j.1346-8138.1989.tb01279.x

Cited by 5 publications

(2 citation statements)

References 7 publications

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“…The immune cells activated in the initiation phase then circulate throughout the body with lymph and blood circulation. [ 12 ] During the elicitation phase, effector T cells from lymph nodes and blood were recruited at the challenged site. [ 4 ] The animal model used in this experiment was designed to mimic human CD, especially allergic contact dermatitis.…”

Section: Discussionmentioning

confidence: 99%

Decoction of Dictamnus Dasycarpus Turcz. Root bark ameliorates skin lesions and inhibits inflammatory reactions in mice with contact dermatitis

Yang¹,

Lee²,

Kim³

et al. 2017

Phcog Mag

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Background:The root bark of Dictamnus dasycarpus Turcz. (Dictamni Radicis Cortex) has been widely used to treat skin diseases in Korea, and its anti-inflammatory efficacies were recently reported.Objective:The paper aims to investigate the inhibitory effects of decoction of Dictamni Radicis Cortex (DDRC) in mice with contact dermatitis (CD).Materials and Methods:We investigated the effects of DDRC on skin lesion characteristics such as crust, scales, incrustation and petechiae, the erythema and melanin indexes, skin thickness, histopathologic changes, and cytokine production in 1-fluoro-2,4-dinitrofluorobenzene (DNFB)-induced CD mice.Results:Topical application of DDRC ameliorated crust, scales, incrustation, and induced by DNFB. In addition, DDRC lowered the erythema index significantly (P < 0.05). DDRC effectively inhibited enlargement of skin thickness (P < 0.05). Histopathologic observation showed that DDRC inhibited epidermal hyperplasia, hyperkeratosis, and spongiotic changes. Finally, DDRC decreased production levels of IFN-γ, TNF-α and IL-6 induced by repeated application of DNFB (P < 0.05).Conclusion:These data suggest that DDRC can be used in the treatment of inflammatory skin diseases including CD. Moreover, these results are closely related to the decreasing production of TNF-α IFN-γ and IL-6 in inflamed tissues.SUMMARY DDRC ameliorated skin lesions such as crust, scales, incrustation and petechiae, and lowered erythema index on skin surface in CD miceDDRC inhibited enlargement of dorsal skin and prevented epidermal hyperplasia, hyperkeratosis, and spongiotic changes in inflamed tissuesDDRC reduced the levels of TNF-α, IFN-γ, and IL-6 in inflamed tissues of CD miceDDRC did not affect spleen/body weight ratio in CD mice. Abbreviations used: DDRC: decoction of Dictamni Radicis Cortex, CD: contact dermatitis, DNFB: 1-fluoro-2,4-dinitrofluorobenzene, AOO: acetone and olive oil, DEX: dexamethasone, CBA: cytometric bead array

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Section: Discussionmentioning

confidence: 99%

Decoction of Dictamnus Dasycarpus Turcz. Root bark ameliorates skin lesions and inhibits inflammatory reactions in mice with contact dermatitis

Yang¹,

Lee²,

Kim³

et al. 2017

Phcog Mag

View full text Add to dashboard Cite

show abstract

“…portant role in proliferation and differentiation of keratinocytes, Langerhans' cells (LC) and melanocytes (7). Recently, a rô le for PKC in the migration of LC from epidermis and also in the induction of contact hypersensitivity has been demonstrated (8,9). In a preliminary study in our laboratory, a decrease (down-regulation) of mouse skin PKC a, b, g and d protein levels in sodium dodecyl sulfate (SDS) induced ICD was detected.…”

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confidence: 99%

Down‐regulation of protein kinase C isoforms in irritant contact dermatitis

Fiedler

Kumar

1998

Contact Dermatitis

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Protein kinase C (PKC) isoforms are important in cell signal transduction associated with regulation of cell proliferation and differentiation. In this study, alterations of PKC isoform levels in irritant patch test reactions were detected by Western immunoblots. 4 chemically and structurally different irritants, 4% and 8% hydrochloric acid (HCl); 1% and 2% sodium hydroxide (NaOH); 20% and 40% nonanoic acid (NON) in 1-propanol; and 5% and 10% sodium dodecyl sulfate (SDS) were applied on the backs of mice in Finn Chambers and fixed with surgical dressings. The patches were kept on the skin for 24 h and removed. 24 h after removal, mild erythema with thickening of skin without vesicles was observed in all irritated skin, except that 4% HCl and 1% NaOH treated skin showed unremarkable skin reaction. No visible skin reaction was detected in vehicle-treated skin. PKC isoform alpha, beta, gamma, and delta levels in irritated skin revealed a 10% to 65% decrease compared to vehicle treated skin. These results indicate that in HCl, NaOH, NON and SDS-induced irritation, activation of the PKC related cell signal transduction cascade may be involved, and that PKC mediated events may be a common phenomenon in irritant contact dermatitis.

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Protein kinase C isoform levels in normal and sodium dodecyl sulphate-irritated mouse skin

Kumar

1998

Br J Dermatol

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p53 plays an essential part in the maintenance of the cellular genetic stability after a DNA-damaging event such as ultraviolet (UV) radiation. Following UV radiation, the amount of p53 protein is elevated. The increased p53 is believed to induce cell cycle arrest, promote nucleotide excision repair (NER) and apoptosis. To study if cells respond differently to high- and low-dose UV radiation, we examined the DNA repair efficiency and apoptosis rate of human and murine fibroblasts after UV radiation. Using a host cell reactivation assay, we found that NER was increased after low doses but not after high doses of UV radiation. In contrast, apoptosis occurred only after the cells received high doses (over 200 J/m2), but not low doses of UVB. The induction of both NER and apoptosis was observed only in p53+/+ murine fibroblasts, not in p53-/- cells, indicating that both stress response mechanisms are dependent on wild-type p53 function. UV radiation induced the expression of p53 protein in a dose-dependent manner up to 400 J/m2. In contrast, p21waf1/cip1 was induced only after low doses and bax only after high doses of UV radiation, supporting the roles of p21waf1/cip1 and bax in NER and apoptosis, respectively. Taken together, these results indicate that cellular stress response to UV radiation depends on UV dose, DNA repair after low doses and apoptosis after high doses, and that both mechanisms are dependent on wild-type p53 function.

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Flow Cytometric Analysis of Protein Kinase C in Lymph Node Cells during the Induction Phase of Contact Hypersensitivity Reaction

Cited by 5 publications

References 7 publications

Decoction of Dictamnus Dasycarpus Turcz. Root bark ameliorates skin lesions and inhibits inflammatory reactions in mice with contact dermatitis

Decoction of Dictamnus Dasycarpus Turcz. Root bark ameliorates skin lesions and inhibits inflammatory reactions in mice with contact dermatitis

Down‐regulation of protein kinase C isoforms in irritant contact dermatitis

Protein kinase C isoform levels in normal and sodium dodecyl sulphate-irritated mouse skin

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