“L” forms of Staphylococci; Their reversibility; Changes in the sensitivity pattern after several intermediary passages in the “L” phase

Schonfeld, Justin; Haas-Poppinga, H. A. de

doi:10.1007/bf02542857

Cited by 11 publications

(2 citation statements)

References 5 publications

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“…Staphylococcal L forms were first observed, but not successfully subcultured, by Dienes and Sharp (1956). Later, Schonfeld (1959), Prozorovskii (1959), Marston (1961a), and Kagan, MIolander, and Weinberger (1962) produced staphylococcal L forms which grew when transplanted to fresh medium. These investigators used complex media containing horse serum or ascitic fluid together with penicillin oIr other antibiotics.…”

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FACTORS AFFECTING GROWTH OF STAPHYLOCOCCUS AUREUS L FORMS ON SEMIDEFINED MEDIUM

Banville

1964

J Bacteriol

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A semidefined agar medium was found suitable for production and cultivation of the L form of Staphylococcus aureuis. In semidefined liquid medium, growth of the L form took place in the form of a sediment containing large masses of cells, but heavy and diffuse growth occurred in the same medium with 0.05% agar. The optimal pH for L-colony formation on solid medium was 6.5. More L colonies developed on 0.75% agar than at higher agar concentrations. L colonies developed in greater numbers on pour plates than on streak plates, and in some cases more L colonies appeared under anaerobic incuibation. L-colony formation appeared to be inhibited by sodium citrate. The vitamin requirements of the L forms stuidied were similar to those of the classical form.

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FACTORS AFFECTING GROWTH OF STAPHYLOCOCCUS AUREUS L FORMS ON SEMIDEFINED MEDIUM

Banville

1964

J Bacteriol

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“…Since the report of Dienes & Sharp (1956) on the isolation of various organisms in the L-phase by using a medium containing a high salt concentration, serum and penicillin, there have been several studies on the biology of the L-forms, including those of Staphylococcus aureus (Schonfeld, 1959(Schonfeld, , 1961 ; Marston, 1961 a, b; Williams, 1963). Although these reports, particularly the last, gave indirect evidence, such as osmotic fragility, phage and antibiotic insensitivity, for the lack of cell-wall material, no direct evidence was presented.…”

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Cell-Wall Deficiencies in L-forms of Staphylococcus aureus

Pratt¹

1966

Journal of General Microbiology

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SUMMARYThree strains of penicillin-induced and one strain of D-cycloserineinduced L-forms of Staphylococcus aureus were investigated for components of the normal bacterial cell wall. None of the 4 strains was found to contain more than 0.008 yo of its dry weight as muramic acid, indicating the lack of mucopeptide. These forms also lacked the cell-wall ribitol teichoic acid as shown by the failure to yield any material containing ribitol. Neither muramic acid nor ribitol was detected as acid-soluble nucleotides in the L-forms, and teichoic acid was not released into the supernatant medium. Serological evidence for polysaccharide A was not found, but there was serological identity between extracts of membranes obtained from staphylococci and extracts of membranes isolated from the L-forms.

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Some physiological characters of L forms of Staphylococcus aureus

Smith

Willis

1967

J. Pathol.

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Cell membranes of Mycoplasma were isolated either by osmotic lysis or by ultrasonic disruption of the organisms. The membranes were dissolved in phenol-acetic acid-water (2:1:0.5, w/v/v), and membrane proteins were separated electrophoretically in polyacrylamide gels containing 5 m urea and 35% (v/v) acetic acid. The electrophoretic patterns of membrane proteins were highly specific for the different Mycoplasma strains examined. The use of this method to prove the identity or dissimilarity of Mycoplasma strains is suggested.

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“L” forms of Staphylococci; Their reversibility; Changes in the sensitivity pattern after several intermediary passages in the “L” phase

Cited by 11 publications

References 5 publications

FACTORS AFFECTING GROWTH OF STAPHYLOCOCCUS AUREUS L FORMS ON SEMIDEFINED MEDIUM

FACTORS AFFECTING GROWTH OF STAPHYLOCOCCUS AUREUS L FORMS ON SEMIDEFINED MEDIUM

Cell-Wall Deficiencies in L-forms of Staphylococcus aureus

Some physiological characters of L forms of Staphylococcus aureus

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