Some physiological characters of L forms of <i>Staphylococcus aureus</i>

Smith, John A.; Willis, A. T.

doi:10.1002/path.1700940215

Cited by 27 publications

(4 citation statements)

References 18 publications

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“…When the loss of cell wall is obtained by means of growth on penicillin-containing media, several properties are reported to remain unchanged and only a few are lost or changed. Smith and Willis (19) have reported that L-forms of S. aureus resembled their original coccal forms in the qualitative production of catalase, coagulase, deoxyribonuclease, gelatinase, lipase, and colonial pigmentation; fibrinolysin and hemolysin could not be observed. Several authors also report that coagulase production is maintained in the L-forms (11,22).…”

Section: Resultsmentioning

confidence: 98%

Investigation of the Penicillinase Activity in L Colonies of Staphylococcus aureus

Rosdahl¹,

Vejlşgaard²

1970

Applied Microbiology

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Section: Resultsmentioning

confidence: 98%

Investigation of the Penicillinase Activity in L Colonies of Staphylococcus aureus

Rosdahl¹,

Vejlşgaard²

1970

Applied Microbiology

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“…Landman and Spiegelman (15) reported that high concentrations of nucleases were detrimental to the survival of protoplasts of Bacillus megaterium. However, L forms of S. aureus have been found to produce DNase (22), and so have protoplasts of group A streptococci (10).…”

Section: Discussionmentioning

confidence: 99%

Biochemical markers of the penicillin-induced L phase of a group B, type III Streptococcus sp

Flores

Ferrieri²

1983

J Clin Microbiol

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The penicillin-induced L phase of growth of the group B. type III streptococcal strain 76-043 was examined for biochemical properties used for the identification of group B streptococci. After numerous serial subcultures in the cell walldefective state, this stable L phase continued to produce hemolysin. hippuricase, and CAMP factor in addition to the group-and type-specific antigens. Hemolysin production by the L-phase cells was observed on solid and in liquid media containing sheep erythrocytes. Washed whole L-phase cells hydrolyzed hippuric acid. CAMP factor was detected by the characteristic hemolysis produced on blood agar by L-phase cells or filtered culture supernatants. CAMP factor activity was quantitated by an agar well diffusion system and a macrotube assay with partially purified preparations of CAMP factor and staphylococcal beta-hemolysin. Hyperimmune cow serum neutralized the CAMP activity of the L phase and parent bacterial phase to the same degree, suggesting identity of the CAMP factors. Production of hemolysin, hippuricase. and CAMP factor confirmed the bacterial origin of this L phase. Assay for these biological markers could be used to identify L-phase organisms derived from group B streptococci.

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“…The suspensions were disrupted by sonication (thirty 2-s bursts at 200–300 W with a 2-s cooling period between each burst) and then centrifuged at 10,000 × g for 20 min at 4°C. The supernatant was centrifuged at 100,000 × g for 1 h at 4°C to collect the cell membranes [34]. The cell membrane pellet was dissolved in resuspension buffer (10 mM potassium phosphate, 50 mM NaCl, 20% glycerol (v/v)) and stored at -70°C until use.…”

Section: Methodsmentioning

confidence: 99%

A recombinant chimera comprising the R1 and R2 repeat regions of M. hyopneumoniae P97 and the N-terminal region of A. pleuropneumoniaeApxIII elicits immune responses

Lee

Chae

et al. 2014

BMC Vet Res

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BackgroundInfection by Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae, either alone or together, causes serious respiratory diseases in pigs.ResultsTo develop an efficient multi-disease subunit vaccine against these pathogens, we produced a chimeric protein called Ap97, which comprises a deletion derivative of the N-terminal region of the A. pleuropneumoniae ApxIII toxin (ApxN) and the R1 and R2 repeats of M. hyopneumoniae P97 adhesin (P97C), using an E. coli expression system.The levels of both IgG1 and IgG2a isotypes specific for ApxN and P97C in the sera of Ap97-immunized mice increased, and Ap97 induced the secretion of IL-4 and IFN-γ by mouse splenocytes. Antisera from mice and pigs immunized with Ap97 readily reacted with both native ApxIII and P97 proteins. In addition, immunization with the Ap97 vaccine effectively protected pigs against challenge with both pathogens.ConclusionsThese findings suggest that Ap97 confers immunogenicity, and is an effective vaccine that protects pigs against infection by M. hyopneumoniae and A. pleuropneumoniae.

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Some physiological characters of L forms of Staphylococcus aureus

Cited by 27 publications

References 18 publications

Investigation of the Penicillinase Activity in L Colonies of Staphylococcus aureus

Investigation of the Penicillinase Activity in L Colonies of Staphylococcus aureus

Biochemical markers of the penicillin-induced L phase of a group B, type III Streptococcus sp

A recombinant chimera comprising the R1 and R2 repeat regions of M. hyopneumoniae P97 and the N-terminal region of A. pleuropneumoniaeApxIII elicits immune responses

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