FACTORS AFFECTING GROWTH OF
            <i>STAPHYLOCOCCUS AUREUS</i>
            L FORMS ON SEMIDEFINED MEDIUM

Banville, Robert R.

doi:10.1128/jb.87.5.1192-1197.1964

Cited by 12 publications

(3 citation statements)

References 14 publications

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“…Growth of Listeria L forms in liquid media as clumps and discrete colonies covering the agar block inoculum has also been reported by Brem and Eveland (3). Similar growth has been reported for other bacterial L forms (2,4,7). Madoff and Dienes (Bacteriol.…”

Section: Discussionsupporting

confidence: 81%

Listeria monocytogenes L Forms I. Induction, Maintenance, and Biological Characteristics

1968

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L forms were induced from 15 of 16 strains of Listeria monocytogenes on penicillin gradient plates incubated under aerobic conditions. The culture medium for maintenance of these L forms must contain an electrolyte in a concentration of 1 % or sucrose in a concentration of 10%. The electrolytes NaCl, KCI, or MgSO4 were used in both induction and maintenance media. Induction of L forms occurred more rapidly on media containing KCl. Listeria L forms had the same fermentation reactions as the parent bacterium. The L-form growth in liquid medium was slow, not extensive, and appeared as clumps on the bottom of culture tubes. The morphology of Listeria L forms was similar to that reported for other bacterial L forms. The L forms derived from strain 10403, serotype 1, were stable after two or more passages on penicillin media. They did not revert to the bacterial form after 40 subcultures on penicillin-free media. Some L-form colonies derived from strain 10403 did revert to the bacterial form when transferred directly from induction plates to penicillin-free media. Studies of the growth characteristics for L forms derived from strain 10403 gave the following results: an optimal temperature of 30 C, high electrolyte or sucrose concentration necessary for induction and maintenance, and no requirement for serum.

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Section: Discussionsupporting

confidence: 81%

Listeria monocytogenes L Forms I. Induction, Maintenance, and Biological Characteristics

1968

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“…One hypothesis is that reflux may be affecting the mucosal environment and changing the composition of the laryngeal microbiome. However, Staphylococcus aureus does not grow well at pH environments less than 5.5, 16 making a link between reflux and MSSA/MRSA laryngitis less likely. Additionally, comparing the laryngeal microbiome of patients with and without reflux demonstrated no change in microbial diversity or composition.…”

Section: Discussionmentioning

confidence: 99%

Factors Associated With Infectious Laryngitis: A Retrospective Review of 15 Cases

Thomas

Jetté

Clary

2017

Ann Otol Rhinol Laryngol

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Objectives To identify the culturable microbes associated with infectious laryngitis and outline effective treatment strategies. Methods This is a retrospective chart review of adult patients with persistent dysphonia plus evidence of laryngeal inflammation who underwent biopsy for culture at a tertiary care medical center. Demographic factors, symptoms as reported on validated patient assessment tools, past medical history, social history, culture results, and treatment duration and response were reviewed. Results Fifteen patients with infectious laryngitis were included in this study. Culture results demonstrated Methicillin-sensitive Staphylococcus aureus (MSSA), Methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, Serratia marcescens, and “normal respiratory flora.” In most patients, multiple courses of prolonged antibiotics were needed to treat MSSA or MRSA. Infections associated with other microbes resolved with a single course of antibiotics. Conclusions In this population, infectious laryngitis is defined as colonization with bacteria not found in the previously characterized laryngeal microbiome of benign vocal fold lesions. In suspected cases of infectious laryngitis, culture is recommended, by biopsy if needed. For MSSA- and MRSA-associated laryngitis, an extended course of antibiotics may be necessary for symptom improvement and resolution of laryngeal inflammation. However, the optimal treatment regimen has yet to be defined and will require larger, prospective studies.

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“…A semidefined broth medium was compounded by Abrams (1), and a completely synthetic broth was described by Medill and O'Kane (14) for the Proteus L forms. In addition, a Staphylococcus aureus L form (5) and the 3B type L form of Bacillus subtilis (15) have been cultured on semidefined and defined media, respectively. The B. subtilis L form required an agar support.…”

mentioning

confidence: 99%

Induction and Propagation of a Bacillus subtilis L Form in Natural and Synthetic Media

Burmeister

Hesseltine

1968

J Bacteriol

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The L form of Bacillus subtilis NRRL B-3275 was induced in a 7% NaCl broth medium and subsequently propagated in natural and synthetic media. The L form grew readily in tryptone broth supplemented with glucose, NaCl, and phosphate buffer, and in a synthetic medium containing only glucose and biotin, in addition to the required salts. Successive transfers from the bacillus inoculum and subsequent large bodies in the tryptone broth with 7% NaCl resulted in gradual selection or transition from the bacillary form to a stable L form without the addition of an antibiotic. The number of viable granules attained in the broth culture exceeded 9 X 107 per ml, and numerous large bodies were always present in rapidly growing cultures.

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FACTORS AFFECTING GROWTH OF STAPHYLOCOCCUS AUREUS L FORMS ON SEMIDEFINED MEDIUM

Cited by 12 publications

References 14 publications

Listeria monocytogenes L Forms I. Induction, Maintenance, and Biological Characteristics

Listeria monocytogenes L Forms I. Induction, Maintenance, and Biological Characteristics

Factors Associated With Infectious Laryngitis: A Retrospective Review of 15 Cases

Induction and Propagation of a Bacillus subtilis L Form in Natural and Synthetic Media

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