L-carnitine affects osteoblast differentiation in NIH3T3 fibroblasts by the IGF-1/PI3K/Akt signalling pathway

Ge, Pinglan; Cui, Yazhou; Liu, Fang; Luan, Jing; Zhou, Xiaoyan; Han, Jinxiang

doi:10.5582/bst.2015.01000

Cited by 22 publications

(18 citation statements)

References 44 publications

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“…However, we saw that L‐carnitine correlated negatively with levels of IGF‐1 as well as MUPs, although we measured L‐carnitine in the liver. A study using mouse embryonic fibroblasts showed that although low levels of L‐carnitine activated the IGF‐1/PI3K/Akt pathways, high levels of L‐carnitine decreased the expression of IGF‐1 and significantly inhibited the pathway (Ge et al ., 2015). This may indicate that carnitine regulation of IGF‐1/PI3K/Akt pathways is dose dependent.…”

Section: Discussionmentioning

confidence: 99%

The effects of graded levels of calorie restriction: IX. Global metabolomic screen reveals modulation of carnitines, sphingolipids and bile acids in the liver of C57BL/6 mice

et al. 2017

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SummaryCalorie restriction (CR) remains the most robust intervention to extend lifespan and improve health span. Using a global mass spectrometry‐based metabolomic approach, we identified 193 metabolites that were significantly differentially expressed (SDE) in the livers of C57BL/6 mice, fed graded levels of CR (10, 20, 30 and 40% CR) compared to mice fed ad libitum for 12 h a day. The differential expression of metabolites also varied with the different feeding groups. Pathway analysis revealed that graded CR had an impact on carnitine synthesis and the carnitine shuttle pathway, sphingosine‐1‐phosphate (S1P) signalling and methionine metabolism. S1P, sphingomyelin and L‐carnitine were negatively correlated with body mass, leptin, insulin‐like growth factor‐ 1 (IGF‐1) and major urinary proteins (MUPs). In addition, metabolites which showed a graded effect, such as ceramide, S1P, taurocholic acid and L‐carnitine, responded in the opposite direction to previously observed age‐related changes. We suggest that the modulation of this set of metabolites may improve liver processes involved in energy release from fatty acids. S1P also negatively correlated with catalase activity and body temperature, and positively correlated with food anticipatory activity. Injecting mice with S1P or an S1P receptor 1 agonist did not precipitate changes in body temperature, physical activity or food intake suggesting that these correlations were not causal relationships.

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Section: Discussionmentioning

confidence: 99%

The effects of graded levels of calorie restriction: IX. Global metabolomic screen reveals modulation of carnitines, sphingolipids and bile acids in the liver of C57BL/6 mice

et al. 2017

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“…The insulin-like growth factor (IGF) axis is an important growth-regulatory pathway that is prevalent in a variety of cancer types, including NSCLC [36]. It has been reported that IGF-1 plays an important role in the activation of the IGF-1/PI3K/Akt signaling pathway [37]. Our study found that compared with the A549 cells in the IGF-1 siRNA group, while those in the miR-135a inhibitors + IGF-1 siRNA group had higher proliferation, invasion, and migration as well as reduced apoptosis, which suggested that miR-135a resulted in the inhibition of the IGF-1/PI3K/Akt signaling pathway to suppress NSCLC cells.…”

Section: Discussionmentioning

confidence: 99%

Effect of microRNA-135a on Cell Proliferation, Migration, Invasion, Apoptosis and Tumor Angiogenesis Through the IGF-1/PI3K/Akt Signaling Pathway in Non-Small Cell Lung Cancer

Zhou

et al. 2017

Cell Physiol Biochem

112

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Objective: This study explored the ability of microRNA-135a (miR-135a) to influence cell proliferation, migration, invasion, apoptosis and tumor angiogenesis through the IGF-1/PI3K/Akt signaling pathway in non-small cell lung cancer (NSCLC). Methods: NSCLC tissues and adjacent normal tissues were collected from 138 NSCLC patients. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression levels of miR-135a and IGF-1, PI3K, Akt, VEGF, bFGF and IL-8 mRNA; western blotting was used to determine the expression levels of IGF-1, PI3K and Akt protein; and enzyme-linked immunosorbent assay (ELISA) was used to analyze the expression levels of VEGF, bFGF and IL-8 protein. Human NSCLC cell lines (A549, H460, and H1299) and the human bronchial epithelial cell line (HBE) were selected. A549 cells were assigned to blank, negative control (NC), miR-135a mimics, miR-135a inhibitors, IGF-1 siRNA and miR-135a inhibitors + IGF-1 siRNA groups. The following were performed: an MTT assay to assess cell proliferation, a scratch test to detect cell migration, a Transwell assay to measure cell invasion, and a flow cytometry to analyze cell apoptosis. Results: The expression level of miR-135a was lower while those of IGF-1, PI3K and Akt mRNA were higher in NSCLC tissues than in the adjacent normal tissues. Dual-luciferase reporter assay indicated IGF-1 as a target of miR-135a. The in vitro results showed that compared with the blank group, cell proliferation, migration and invasion were suppressed, mRNA and protein levels of IGF-1, PI3K, Akt, VEGF, bFGF and IL-8 were reduced, and cell apoptosis was enhanced in the miR-135a mimics and IGF-1 siRNA groups. Compared with the IGF-1 siRNA group, cells in the miR-135a inhibitors + IGF-1 siRNA group demonstrated increased cell proliferation, migration and invasion, elevated mRNA and protein levels of IGF-1, PI3K, Akt, VEGF, bFGF and IL-8 and reduced cell apoptosis. Conclusion: These findings indicated that miR-135a promotes cell apoptosis and inhibits cell proliferation, migration, invasion and tumor angiogenesis by targeting IGF-1 gene through the IGF-1/PI3K/Akt signaling pathway in NSCLC.

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“…After treatment with siRNA transfection and osteoblast induction, ALP expression was assayed by means of an ALP activity staining kit (GenMed Scientifics Inc., USA), and the activity was measured as described previously (13). Briefly, lysis buffer (25 mM Tris-HCL, 0.5% TritonX-100) was added to precipitated SaOS2 cells and MVs to release ALP at 4°C for 1 h respectively, and then after incubating with p-nitrophenyl phosphate (p-NPP) substrate at 37°C for 20 min, the absorbance of the mixture at 405 nm was measured using a micro plate reader.…”

Section: Alkaline Phosphatase (Alp) Activity Alizarinred Staining Amentioning

confidence: 99%

Tenascin C affects mineralization of SaOS2 osteoblast-like cells through matrix vesicles

Cui

Luan

et al. 2016

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L-carnitine affects osteoblast differentiation in NIH3T3 fibroblasts by the IGF-1/PI3K/Akt signalling pathway

Cited by 22 publications

References 44 publications

The effects of graded levels of calorie restriction: IX. Global metabolomic screen reveals modulation of carnitines, sphingolipids and bile acids in the liver of C57BL/6 mice

The effects of graded levels of calorie restriction: IX. Global metabolomic screen reveals modulation of carnitines, sphingolipids and bile acids in the liver of C57BL/6 mice

Effect of microRNA-135a on Cell Proliferation, Migration, Invasion, Apoptosis and Tumor Angiogenesis Through the IGF-1/PI3K/Akt Signaling Pathway in Non-Small Cell Lung Cancer

Tenascin C affects mineralization of SaOS2 osteoblast-like cells through matrix vesicles

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