Tenascin C affects mineralization of SaOS2 osteoblast-like cells through matrix vesicles

Li, Chengzhi; Cui, Yazhou; Luan, Jing; Zhou, Xiaoyan; Li, Haiying; Wang, Huaxin; Shi, Liang; Han, Jinxiang

doi:10.5582/ddt.2016.01009

Cited by 17 publications

(13 citation statements)

References 23 publications

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“…One of the recently detected cell surface proteoglycans that is carried by EV is glypican-1, which is enriched on cancer cell-derived EV and is one of the most promising EV markers in the early diagnostics of pancreatic cancer [54]. An ECM glycoprotein tenascin C carried by MV regulates bone mineralization activity [55]. Enzymatically active lysyl oxidase-like 2, which is carried on the surface of EV derived from hypoxic endothelial cells, facilitates collagen crosslinking in the ECM [56].…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

Extracellular vesicles are integral and functional components of the extracellular matrix

et al. 2019

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Extracellular vesicles (EV) are small plasma membrane-derived particles released into the extracellular space by virtually all cell types. Recently, EV have received increased interest because of their capability to carry nucleic acids, proteins, lipids and signaling molecules and to transfer their cargo into the target cells. Less attention has been paid to their role in modifying the composition of the extracellular matrix (ECM), either directly or indirectly via regulating the ability of target cells to synthesize or degrade matrix molecules. Based on recent results, EV can be considered one of the structural and functional components of the ECM that participate in matrix organization, regulation of cells within it, and in determining the physical properties of soft connective tissues, bone, cartilage and dentin. This review addresses the relevance of EV as specific modulators of the ECM, such as during the assembly and disassembly of the molecular network, signaling through the ECM and formation of niches suitable for tissue regeneration, inflammation and tumor progression. Finally, we assess the potential of these aspects of EV biology to translational medicine.

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Section: Accepted M Manuscriptmentioning

confidence: 99%

Extracellular vesicles are integral and functional components of the extracellular matrix

et al. 2019

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“…After mineralization in bone matrix, deposition of calcium around bone tissue can be detected by calcium staining experiments, including Alizarin Red S 7 . The mineralization of bone matrix is mainly promoted by induction of collagen type I alpha 1 chain (COL1A1), 8 osteocalcin (OC), 9 osteopontin (OPN), 10 bone sialoprotein (BSP), 11 transforming growth factors‐β (TGF‐β), 12 and bone morphogenic proteins (BMPs) 13 . Moreover, Runt‐related transcription factor 2 (RUNX2), 14 osterix (OSX), 15 distal‐less homeobox 5 (DLX5), 16 and β‐catenin 17 are known to play a pivotal role in osteoblast differentiation.…”

Section: Introductionmentioning

confidence: 99%

Cannabidiol induces osteoblast differentiation via angiopoietin1 and p38 MAPK

Kang

Lee

Park

2020

Environmental Toxicology

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In this study, we report the potential of cannabidiol, one of the major cannabis constituents, for enhancing osteoblastic differentiation in U2OS and MG‐63 cells. Cannabidiol increased the expression of Angiopoietin1 and the enzyme activity of alkaline phosphatase in U2OS and MG‐63. Invasion and migration assay results indicated that the cell mobility was activated by cannabidiol in U2OS and MG‐63. Western blotting analysis showed that the expression of tight junction related proteins such as Claudin1, Claudin4, Occuludin1, and ZO1 was increased by cannabidiol in U2OS and MG‐63. Alizarin Red S staining analysis showed that calcium deposition and mineralization was enhanced by cannabidiol in U2OS and MG‐63. Western blotting analysis indicated that the expression of osteoblast differentiation related proteins such as distal‐less homeobox 5, bone sialoprotein, osteocalcin, type I collagen, Runt‐related transcription factor 2 (RUNX2), osterix (OSX), and alkaline phosphatase was time dependently upregulated by cannabidiol in U2OS and MG‐63. Mechanistically, cannabidiol‐regulated osteoblastic differentiation in U2OS and MG‐63 by strengthen the protein‐protein interaction among RUNX2, OSX, or the phosphorylated p38 mitogen‐activated protein kinase (MAPK). In conclusion, cannabidiol increased Angiopoietin1 expression and p38 MAPK activation for osteoblastic differentiation in U2OS and MG‐63 suggesting that cannabidiol might provide a novel therapeutic option for the bone regeneration.

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“…Tenascin-like, tnc, titin and fras1 related extracellular matrix 3 precursor were identified as specific differentially expressed proteins in 1-IB-vs-2-IB. Tenascin C (TNC) is an extracellular matrix glycoprotein synthesized by osteoblasts during bone growth and morphogenesis, which was reported to affect mineralization of osteoblast-like cells by matrix vesicles [ 45 – 47 ]. Powers et al study had proved that titin protein made an effect on skeletal muscle sarcomeres [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative proteomics analysis of teleost intermuscular bones and ribs provides insight into their development

et al. 2017

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BackgroundIntermuscular bones (IBs) and ribs both are a part of skeletal system in teleosts, but with different developing process. The chemical composition of fish IBs and ribs as well as the underlying mechanism about their development have not been investigated. In the present study, histological structures showed that one bone cavity containing osteoclasts were existed in ribs, but not in IBs of Megalobrama amblycephala. We constructed the first proteomics map for fish bones including IBs and ribs, and identified the differentially expressed proteins between IBs and ribs through iTRAQ LC-MS/MS proteomic analysis.ResultsThe proteins extracted from IBs and ribs at 1- to 2-year old M. amblycephala were quantified 2,342 proteins, with 1,451 proteins annotated with GO annotation in biological processes, molecular function and cellular component. A number of bone related proteins as well as pathways were identified in the study. A total of 93 and 154 differently expressed proteins were identified in comparison groups of 1-IB-vs-1-Rib and 2-IB-vs-2-Rib, which indicated the obvious differences of chemical composition between these two bone tissues. The two proteins (vitronectin b precursor and matrix metalloproteinase-2) related to osteoclasts differentiation were significantly up-regulated in ribs compared with IBs (P < 0.05), which was in accordance with the results from histological structures. In comparison groups of 1-IB-vs-2-IB and 1-Rib-vs-2-Rib, 33 and 51 differently expressed proteins were identified and the function annotation results showed that these proteins were involved in regulating bone development and differentiation. Subsequently, 11 and 13 candidate proteins in comparison group of 1-IB-vs-1-Rib and 1-IB-vs-2-IB related to bone development were validated by MRM assays.ConclusionsOur present study suggested the different key proteins involved in the composition of fish ribs and IBs as well as their growth development. These findings could provide important clues towards further understanding of fish skeletal system and the roles of proteins playing in regulating diverse biological processes in fish.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-017-3530-z) contains supplementary material, which is available to authorized users.

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Tenascin C affects mineralization of SaOS2 osteoblast-like cells through matrix vesicles

Cited by 17 publications

References 23 publications

Extracellular vesicles are integral and functional components of the extracellular matrix

Extracellular vesicles are integral and functional components of the extracellular matrix

Cannabidiol induces osteoblast differentiation via angiopoietin1 and p38 MAPK

Comparative proteomics analysis of teleost intermuscular bones and ribs provides insight into their development

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