Kualitas semen dan produksi semen beku pada bangsa sapi dan bulan penampungan yang berbeda

Rahmawati, Mita Ayu; Susilawati, Trinil; Ihsan, Muhammad Nur

doi:10.21776/ub.jiip.2015.025.03.04

Cited by 11 publications

(14 citation statements)

References 3 publications

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“…The decrease was within the reasonable limit which, according to Parrish (2003), was 10-40%, and 33.27 ± 5.57% in Pasundan Cattle (Baharun et al, 2017). Mostari et al (2004) reported that different breeds showed varied percentage of motility due to differences in energy source composition in seminal plasma (Rahmawati et al, 2015). Accordingly, the declining motility was influenced by breeds as a semen producer.…”

Section: Effect Of Breeds On Semen Qualitymentioning

confidence: 86%

The Influence of Breed and Type of Extender on the Quality of Bull Semen

Sukirman¹,

Sukmawati²,

Rasad

et al. 2020

anprod

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This study aimed to determine the influence of breed and type of extenders on frozen semen quality of cows at BIB Lembang. The experimental study was conducted in a Factorial Randomized Block Design (RBD) with two factors. The first factor was four cow breeds, i.e. Ongole Cross (PO), Brahman (BR), Simmental (SM) and Limousin (LM), and the second factor was two types of extender, i.e. Skim-Egg Yolk (SKT) and AndroMed® (AND), all repeated four times. The observed variables were percentage of spermatozoa motility and intact plasma membrane (IPM). All data obtained were analyzed using a general linear model (IBM SPSS ver. 23). The results demonstrated an interaction between breed and the type of diluent to motility. Breeds showed significantly different motility but non-significantly different intact plasma membrane (MPU) of semen. The type of diluent did not significantly affect motility and intact plasma membrane (MPU) of the frozen semen. The effect of the breed on BR motility was lower and significantly different from PO, LM and SM. The types of diluent did not significantly affect motility, MPU. The results showed that SKT was lower than AND, it was indicative effect of breed on intact plasma membrane (MPU) PO was lower than BR, LM and SM and the effect of the type of diluent on whole plasma membrane (MPU) AND is lower than SKT. It can be concluded that breed influences the motility of semen. The lowest motility reduction in frozen semen is Brahman cattle by using skim-egg yolk extender.

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Section: Effect Of Breeds On Semen Qualitymentioning

confidence: 86%

The Influence of Breed and Type of Extender on the Quality of Bull Semen

Sukirman¹,

Sukmawati²,

Rasad

et al. 2020

anprod

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“…Pada penelitian ini volume semen sapi limousin berada pada kisaran yang normal. Volume semen hasil penelitian ini lebih rendah dibandingkan volume semen sapi limousin hasil penelitian Rahmawati et al (2015) sebanyak ratarata 6,73 ml dan Wiratri et al(2014) sebanyak ratarata 6,75 ml. Namun demikian, ada beberapa hasil penelitian yang melaporkan volume semen lebih rendah daripada hasil penelitian ini antara lain 3,2-7,3 ml (Sumeidiana et al, 2007;Hartanti et al, 2012) dan 4-8 ml (Arifiantini, 2012).…”

Section: Hasil Dan Pembahasan Karakteristik Semen Segar Sap Limousinunclassified

Daya Hidup Spermatozoa Sapi Limousin yang Dipreservasi dengan Pengencer Tris dan Berbagai Konsentrasi Sari Kedelai

Coester¹,

Sulaiman²,

Rizal³

2019

jitro

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ABSTRAK digunakan sebagai salah satu bahan pengencer semen pengganti kuning telur ayam. Penelitian ini bertujuan untuk menguji pengaruh sari kedelai dalam pengencer Tris terhadap motilitas dan daya hidup spermatozoa sapi limousin yang dipreservasi pada suhu 5oC. Semen ditampung dengan vagina buatan. Semen segar yang memenuhisyarat dibagi kedalam empat buah tabung reaksi yang masing-masing berisi pengencer perlakuan, yakni: 80% pengencer dasar Tris + 20% kuning telur (Tris), 97% pengencer dasar Tris + 3% sari kedelai (SK3), 95% pengencer dasar Tris + 5% sari kedelai (SK5), dan 93% pengencer dasar Tris + 7% sari kedelai (SK7). Semen yang telah diencerkan disimpan di dalam refrigerator pada suhu 5oC, dan dievaluasi motilitas dan viabilitas spermatozoa setiap hari hingga hari kelima. Hasil penelitian menunjukkan bahwa motilitas dan viabilitas spermatozoa di dalam pengencer Tris nyata (P<0,05) lebih tinggi dibandingkan dengan spermatozoa di dalam pengencer SK3, SK5, dan SK7 selama empat hari penyimpanan. Berdasarkaan hasil penelitian dapat disimpulkan bahwa pengencer Tris-kuning telur lebih baik dalam mempertahankan motilitas dan daya hidup spermatozoa sapi limousin dibandingkan dengan pengencer Tris-sari kedelai yang dipreservasi pada suhu 5°C. Pengencer Tris dengan konsentrasi 3% sari kedelai lebih baik dibandingkan dengan konsentrasi 5% dan 7%.Kata Kunci : sapi limousine, sari kedelai, semen, trisABSTRACTSoybean contains anlecithin (phosphatidyl choline), that has the potential to be used as substitute for chicken egg yolk as one of the semen extender compound. The aim of this research was to examine the effect of soybean juice in Tris extender on the motility and viability of Limousin cattle spermatozoa preserved at 5oC. Semen was collected using artificial vagina. Fresh semen was divided into four tubes containing a treatment extender, i.e. 80% Tris base extender + 20 egg yolk (Tris), 97% Tris-based extender + 3% soybean juice (SJ3), 95% Tris-based extender + 5% soybean juice (SJ5), 93% Tris-based extender + 7% soybean juice (SJ7), respectively. Diluted-semen was preserved in refrigerator at 5oC, and evaluation of spermatozoa motility and viability were conducted on daily basis up to five days. The result showed that percentages of motility and viability of spermatozoa in Tris-yolk extender were significantly (P<0.05) higher than spermatozoa in SJ3, SJ5, and SJ7extenders during four days of storage. In conclusion, Tris-yolk extender is better than Tris-soybean juice in maintaining the spermatozoa motility and viability of Limousin cattle preserved at 5°C. Tris extender containing 3% soybean juice is better than 5% and 7%.Keywords: limousin cattle, semen, soybean juice, tris

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“…Keywords: Artificial insemination, sexual behavior, sperm production, Brahman bulls (Abidin, 2002) dan termasuk tipe pedaging yang tumbuh cepat di daerah tropis karena sapi ini tahan terhadap panas, gigitan caplak, dan mudah beradaptasi terhadap pakan kualitas jelek (Sudarmono, 2008). Bangsa sapi Brahman memiliki volume, persentase motilitas individu, dan konsentrasi semen yang relatif tinggi, sehingga dapat memproduksi straw semen beku lebih tinggi dibandingkan bangsa sapi yang lain (Rahmawati et al, 2016). Berdasarkan hal tersebut, sapi Brahman mempunyai potensi dikembangkan untuk membantu meningkatkan produksi daging sapi di Indonesia.…”

Section: Hubungan Antara Tingkah Laku Seksual Dengan Produksi Spermatunclassified

Hubungan antara Tingkah Laku Seksual dengan Produksi Spermatozoa Sapi Brahman

et al. 2018

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ABSTRAK. Pejantan untuk program inseminasi buatan adalah pejantan unggul yang diseleksi berdasarkan libido dan produksi spermatozoa. Penelitian bertujuan untuk menganalisa hubungan antara tingkah laku seksual dengan produksi spermatozoa sapi Brahman. Materi penelitian adalah 3 pejantan sapi Brahman yang ditampung menggunakan vagina buatan. Metode penelitian adalah observasional dengan mengamati waktu reaksi dan lama ejakulasi. Observasi laboratorium dengan mengamati volume, konsentrasi, dan motilitas individu untuk menghitung total spermatozoa (TS) dan total spermatozoa motil (TSM). Pengamatan dilakukan sebanyak 10 ulangan. Data dianalisa secara deskriptif serta hubungan antara tingkah laku seksual dan produksi spermatozoa dihitung dengan korelasi Pearson. Hasil penelitian menunjukkan terdapat variasi tingkah laku seksual dan produksi spermatozoa pada sapi Brahman. Individu Cipta mempunyai waktu reaksi dan lama ejakulasi paling singkat serta produksi spermatozoa paling rendah. Hubungan signifikan antara waktu reaksi dengan TS serta TSM pada individu Bizzyard dengan koefisien determinasi (R2) sebesar 14 % dan 20%. Hubungan sangat signifikan antara lama ejakulasi dengan TS serta TSM pada individu Cipta dengan R2 sebesar 46% dan 58%. Kesimpulan penelitian adalah terdapat hubungan antara tingkah laku seksual dengan produksi spermatozoa pada sapi Brahman, dengan koefisien korelasi (r) tertinggi sebesar 0,72 dan R2 sebesar 58% antara lama ejakulasi dan total spermatozoa motil pada individu Cipta. (The relationship between sexual behavior and semen production in Brahman bulls) ABSTRACT. Bulls for artificial insemination must be selected based on libido and sperm production. The study aimed to analyze the relationship between sexual behavior with sperm production on Brahman bulls. The material was 3 Brahman bulls collected using artificial vagina. The method was observational by observing reaction time and ejaculation duration. Laboratory observations by observing volume, concentration, and motility to calculate total sperm (TS) and total motile sperm counts (TMSC). Observations were replicated 10 times. The data were analyzed descriptively, the relationship between behavior and sperm production was examined using Pearson correlation. The results showed that there were variations in sexual behavior and sperm production in each bull. Cipta have the shortest reaction time and ejaculation duration and the lowest sperm production. A significant relationship between reaction time and TS and TMSC in Bizzyard with coefficient of determination (R2) of 14% and 20%. A very significant between ejaculation duration with TS and TMSC in Cipta with R2 of 46% and 58%. In conclusion, there was a relationship between sexual behavior and sperm production in Brahman bulls, with the highest correlation coefficient (r) of 0.72 and R2 of 58% between ejaculation duration and the total motile sperm counts in Cipta.

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Kualitas semen dan produksi semen beku pada bangsa sapi dan bulan penampungan yang berbeda

Cited by 11 publications

References 3 publications

The Influence of Breed and Type of Extender on the Quality of Bull Semen

The Influence of Breed and Type of Extender on the Quality of Bull Semen

Daya Hidup Spermatozoa Sapi Limousin yang Dipreservasi dengan Pengencer Tris dan Berbagai Konsentrasi Sari Kedelai

Hubungan antara Tingkah Laku Seksual dengan Produksi Spermatozoa Sapi Brahman

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