Krüppel-like factor 5-induced overexpression of long non-coding RNA DANCR promotes the progression of cervical cancer via repressing microRNA-145-3p to target ZEB1

Hu, Chunyan; Yu, Han; Zhu, Genhai; Li, Guifei; Wu, Xiurong

doi:10.1080/15384101.2021.1941625

Cited by 6 publications

(3 citation statements)

References 39 publications

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“…hsa-miR-21-5p (MIMAT0000076) [22][23][24] hsa-miR-29b-3p (MIMAT0000100) [25][26][27] hsa-miR-145-5p (MIMAT0000437) [28][29][30] hsa-miR-451a-5p (MIMAT0001631) [31][32][33] hsa-miR-1246-5p (MIMAT0005898) [34][35][36] hsa-miR-1290-3p (MIMAT0005880) [37,38] 2. Results…”

Section: Mirna/id Referencesmentioning

confidence: 99%

“…References hsa-miR-21-5p (MIMAT0000076) [22][23][24] hsa-miR-29b-3p (MIMAT0000100) [25][26][27] hsa-miR-145-5p (MIMAT0000437) [28][29][30] hsa-miR-451a-5p (MIMAT0001631) [31][32][33] hsa-miR-1246-5p (MIMAT0005898) [34][35][36] hsa-miR-1290-3p (MIMAT0005880) [37,38] All these developments were used to create a new method for the cervical dysplasia evaluation based on an analysis of six CC-associated miRNAs. Additionally, a "random forest" machine learning algorithm was applied to improve the interpretation of the results.…”

Section: Mirna/idmentioning

confidence: 99%

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NOVAprep-miR-Cervix: New Method for Evaluation of Cervical Dysplasia Severity Based on Analysis of Six miRNAs

Kniazeva¹,

Zabegina²,

Shalaev³

et al. 2023

IJMS

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Cervical cancer is one of the most common gynecological malignancies and it is preventable through the yearly diagnosis and management of pre-cancerous cervical disease. The profile of miRNA expression in cervical epithelium cells is altered with cervical dysplasia development and further progression. The NOVAprep-miR-CERVIX is a new approach for the assessment of cervical dysplasia through the analysis of six marker miRNAs. This study aims to evaluate theperformance and diagnostic potency of the new method. Cytological smears from 226 women (NILM, n.114; HSIL, n.112) were included in the study. A VPH test was performed with RealBest DNAHPV HR screen Kit, six marker miRNAs (miR-21, -29b, -145, -451a, -1246, -1290) were assayed using NOVAprep-miR-CERVIX kit. Obtained data were analyzed using the Delta Ct method and random forest machine learning algorithm. The results of the quantitative analysis of six microRNAs were expressed as a miR-CERVIX parameter, which ranged from 0 to 1, where “0” corresponded to the healthy cervical epithelium, while “1” corresponded to high-grade squamous intraepithelial dysplasia. The average value of miR-CERVIX differed in groups of NILM and HSIL samples (0.34 vs. 0.72; p < 0.000005). An estimation of miR-CERVIX allowed for the differentiation between healthy and pre-cancerous samples with sensitivity of 0.79 and specificity of 0.79, as well as to confirm HSIL with specificity of 0.98. Interestingly, the HSIL group included HPV(+) and HPV(−) samples, which were statistically significantly different in terms of miR-CERVIX value. Analysis of CC-associated miRNAs in material of cervical smear might serve as an additional method for the evaluation of cervical dysplasia severity.

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Section: Mirna/id Referencesmentioning

confidence: 99%

Section: Mirna/idmentioning

confidence: 99%

NOVAprep-miR-Cervix: New Method for Evaluation of Cervical Dysplasia Severity Based on Analysis of Six miRNAs

Kniazeva¹,

Zabegina²,

Shalaev³

et al. 2023

IJMS

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“…For instance, the miR-145-3p/HDAC4 axis hampers osteosarcoma cell proliferation and promotes autophagy and apoptosis [8]. miR-145-3p represses malignant behaviors of cervical cancer cells by targeting ZEB1 [9]. miR-145-3p restrains the growth of prostate cancer cells by targeting MTDH [10].…”

Section: Introductionmentioning

confidence: 99%

miR-145-3p Hampers the Malignant Progression of Esophageal Carcinoma via CXCL5 Downregulation

Chen

Teng

Zhu

et al. 2022

Analytical Cellular Pathology

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Esophageal carcinoma (EC) is the most prevalent malignant tumor that occurs frequently worldwide. The early diagnostic biomarkers are crucial for EC treatment. miRNA can regulate EC progression, with diagnostic and prognostic value. Herein, differentially expressed miRNAs and mRNAs (DEmRNAs) in EC were predicted based on TCGA database. The target mRNAs of miRNA were predicted through databases, which were then intersected with DEmRNAs. Next, the correlation between miRNA and candidate mRNAs was analyzed. qRT-PCR was introduced to analyze expression of miR-145-3p and CXCL5 mRNA in EC cell lines, and western blot was performed to assess protein expression of CXCL5. Cell proliferation, migration, invasion, and apoptosis in EC were examined through CCK-8, wound healing, Transwell invasion, and flow cytometry assays. Moreover, targeting relationship between miR-145-3p and CXCL5 was verified through luciferase reporter gene analysis. The experimental results revealed a decreased miR-145-3p expression and an increased CXCL5 expression in EC. Enforced expression of miR-145-3p hindered proliferation, migration, invasion, and stimulated apoptosis of EC cells by repressing CXCL5. This study manifested that miR-145-3p may be a tumor suppressor in EC, and miR-145-3p/CXCL5 axis restrained the malignant progression of EC. These results supply an underlying target for prognosis and treatment of EC patients.

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Autophagy as a self-digestion signal in human cancers: Regulation by microRNAs in affecting carcinogenesis and therapy response

Kadhim

Ramírez‐Coronel

Jalil

et al. 2023

Pharmacological Research

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Krüppel-like factor 5-induced overexpression of long non-coding RNA DANCR promotes the progression of cervical cancer via repressing microRNA-145-3p to target ZEB1

Cited by 6 publications

References 39 publications

NOVAprep-miR-Cervix: New Method for Evaluation of Cervical Dysplasia Severity Based on Analysis of Six miRNAs

NOVAprep-miR-Cervix: New Method for Evaluation of Cervical Dysplasia Severity Based on Analysis of Six miRNAs

miR-145-3p Hampers the Malignant Progression of Esophageal Carcinoma via CXCL5 Downregulation

Autophagy as a self-digestion signal in human cancers: Regulation by microRNAs in affecting carcinogenesis and therapy response

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