miR-145-3p Hampers the Malignant Progression of Esophageal Carcinoma via CXCL5 Downregulation

Abstract

Esophageal carcinoma (EC) is the most prevalent malignant tumor that occurs frequently worldwide. The early diagnostic biomarkers are crucial for EC treatment. miRNA can regulate EC progression, with diagnostic and prognostic value. Herein, differentially expressed miRNAs and mRNAs (DEmRNAs) in EC were predicted based on TCGA database. The target mRNAs of miRNA were predicted through databases, which were then intersected with DEmRNAs. Next, the correlation between miRNA and candidate mRNAs was analyzed. qRT-PCR was introduced to analyze expression of miR-145-3p and CXCL5 mRNA in EC cell lines, and western blot was performed to assess protein expression of CXCL5. Cell proliferation, migration, invasion, and apoptosis in EC were examined through CCK-8, wound healing, Transwell invasion, and flow cytometry assays. Moreover, targeting relationship between miR-145-3p and CXCL5 was verified through luciferase reporter gene analysis. The experimental results revealed a decreased miR-145-3p expression and an increased CXCL5 expression in EC. Enforced expression of miR-145-3p hindered proliferation, migration, invasion, and stimulated apoptosis of EC cells by repressing CXCL5. This study manifested that miR-145-3p may be a tumor suppressor in EC, and miR-145-3p/CXCL5 axis restrained the malignant progression of EC. These results supply an underlying target for prognosis and treatment of EC patients.

MicroRNAs: A novel signature in the metastasis of esophageal squamous cell carcinoma

“…The receptor tyrosine kinases (RTKs) signaling pathway effect many signals, including epidermal growth factor (EGF), fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), insulin-like growth factor (IGF) and their receptors, as well as intracellular signaling pathways, such as mitogen-activated protein kinases (MAPKs) and phosphatidylinositol 3-kinase (PI3K). The dysregulation of miRNAs on the RTK signaling pathway is play important roles in ESCC metastasis[ 10 , 12 , 13 , 15 , 23 , 24 , 26 , 30 - 33 , 36 , 39 , 44 , 50 , 53 , 62 , 63 , 66 , 69 , 70 - 73 , 76 - 78 , 80 , 81 , 83 , 85 , 86 , 88 - 91 , 93 , 95 , 97 , 100 - 104 , 107 - 109 , 112 , 117 , 123 , 125 , 128 , 140 - 144 , 146 , 151 , 154 , 160 , 163 , 169 , 174 , 175 , 178 - 180 , 185 - 187 , 189 , 192 , 193 , 195...…”