Korelasi Kandungan Antosianin Total dengan Parameter Warna (L*, a*, dan b*) dan Penanda Mikrosatelit pada Beras Hitam Lokal

Abstract

Black rice is considered as a functional food for its high anthocyanin content, expressed as purple-black grain rice color. The study was aimed to determine the correlation between total anthocyanin content with L*, a*, and b* color variables, total anthocyanin content with color morphology and total anthocyanin content with microsatellite markers. Eleven local black rice varieties derived from the Yogyakarta Assesment Institute of Agriculture Technology collections and two varieties of white rice (Situbagendit and Inpari 6 Jete) were planted on plastic pots in green house. The trial was arranged in a completely randomized design with five replications. At three weeks after planting, leaf samples were tak en for DNA isolation and further P CR amplifications using four microsatellite markers (RM 180, RM 220, RM 224 and RM 252). The black rice grains were observed based on color morphology using a chromameter and analyzed for their anthocyanin content. Regression, correlation, and path analyses were conducted to determine the relationship between total anthocyanin content and L* a* b * color variable, color morphology, and microsatellite markers. Results showed that there were correlation between total anthocyanin content and color variables (rL* =-0.568, ra* =-0.561, rb*= 0.844). The total anthocyanin content were also correlated with color scores of morphology and total microsatellite markers (rRM 180 =-0.419, rRM 220 =0.27, rRM 224 =-0.493, rRM 252 =0.265) and color scores of morphology, r =-0.442. Selection of high-anthocyanin content on rice could use the color variable criteria (L* and a* low, and b* high). Microsatellite markers RM 220 and RM 252 can be used as molecullar markers for identification of rice having high anthocyanin content.

Grain color measurement and nutrient content of local pigmented rice from South Sulawesi

“…The correlation between grain color and anthocyanin content was reported: the higher the value of the color parameters L*, a*, and b*, the lower the total anthocyanin content, and vice versa, the lower the value of the color parameters L*,a*, and b* the higher the total anthocyanin content. This finding is understandable because the higher the L* value, the lighter the color [13]. Previous work of [8] also mentioned that anthocyanin was superior in the dark bold color of grains.…”

“…The total amount of anthocyanins in seventeen different kindsof traditional colored rice of South Sulawesi ranged from 2.89 mg CGE/100-gram dry weight to 187.23 mg CGE/100 g dry weight [8]. It has been demonstrated that the nutritional quality of grainproduced by certain traditional landraces is superior to that of grain produced by conventional, modern rice varieties, primarily as a result of their more efficient accumulation of bioactive compounds [9,10] Color variation in pigmented rice was previously studied by [11] in Yogyakarta and [12] in South Sulawesi. They reported variations in colorimetric measurement.…”

Analysis of Black Rice Farming Competitiveness (A Case Study in Merkarwangi Village, Cisayong District, Tasikmalaya Regency, West Java Province)

“…Therefore, black rice can prevent and treat the body from various degenerative diseases (Dewi et al, 2017). In line with the statement of Dewi et al (2017), (Kristamtini, 2009) stated that black rice is a functional food, namely food that naturally or through certain processes contains one or more compounds that are considered to have physiologically beneficial health. Even the Chinese consider black rice to be a very healthy food (Nosowitz, 2015).…”

“…Open Access Kristamtini et al (2017), black rice contains anthocyanins which are antioxidants in the form of vitamin E, B vitamins, magnesium, iron, zinc, and phosphorus. Therefore, black rice can prevent and treat the body from various degenerative diseases (Dewi et al, 2017).…”

Black Rice Bran Extracts and Fractions Containing Cyanidin 3-glucoside and Peonidin 3-glucoside Induce Apoptosis in Human Cervical Cancer Cells

“…One of them is cultivated in the Special Region of Yogyakarta Province (DIY) named as 'Cempo Ireng' (Kristamtini et al, 2013). Previous studies reported that Cempo Ireng contains high level of anthocyanin and antioxidant activity, thus normalize serum lipid profi le in hyperlipidemia rats (Kristamtini et al, 2012;Pratiwi et al, 2014).…”