Konkur Atlarında Egzersiz Öncesi ve Sonrası ADAMTS-4 ve ADAMTS-5 Düzeyleri

Abstract

A disintegrin-like and metalloproteinase with thrombospondin motifs (ADAMTS) proteinase family play an important role in many physiological and physiopathological processes such as the maintenance of locomotor system health in sport horses. In this study, we aimed to determine the changes of ADAMTS-4 and ADAMTS-5 levels in concour horses before and after exercise. The Oldenburg and Selle Français horse-breed types which are healthy, 6-15 years old, around 650-750 kg, and distinct genders were used (n=10). Following the physical examinations, the horses were subjected to 50 minutes of regular exercise program. Blood samples were collected into anticoagulant-free tubes in order to determine ADAMTS-4 and ADAMTS-5 mRNA expression and ELISA levels before and after exercise. There were no differences were observed statistically on ADAMTS-4, neither mRNA expression in spite of 25% downregulated, nor at the ELISA levels. On the other hand, ADAMTS-5 mRNA expression were upregulated 3.88 fold (p<0.01), as well ELISA level significantly increased as 1.2 fold (p<0.01). In conclusion, the increase of the serum ADAMTS-5 levels may be one of the potential biomarkers in the joint disorders. However, it is necessary to investigate more extensively to clarify its action with clinical evidence.