Kodiak � --a successful biological-control product for suppression of soil-borne plant pathogens of cotton

Brannen, Phillip M.; Kenney, D S

doi:10.1038/sj.jim.2900439

Cited by 121 publications

(68 citation statements)

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“…Strains CAU-B946 (Chinese Agricultural University, Beijing, China), NAU-B3 (Nanjing Agricultural University, Nanjing, China) and YAU Y2 (Yunnan Agricultural University, Kunming, China) were isolated as plant-root-associated bacteria from different locations in China and were previously described as representatives of B. amyloliquefaciens . Other plant-associated strains of B. amyloliquefaciens used in this study were NAU B2, NAU B55 and NAU B85 (Nanjing Agricultural University, Nanjing, China), FZB45 (ABiTEP, Berlin; Krebs et al, 1998;Idriss et al 2002), A1/3 (5FZB109; Hofemeister et al, 2004), GB03 (used for biofungicide Kodiak, Bayer Crop Science LP, NC 27709, USA; Brannen & Kenney, 1997), QST713 (isolated from a California peach orchard in 1995, active ingredient of biofungicide Serenade, AgraQuest, CA, USA), FDK21 (S. Kunz, University of Konstanz, Germany; Chen et al, 2009a), UCM B5113 (isolated from soil; red pigmented) and UCM B5044 (isolated from a cotton plant; Ukranian Collection of Microorganisms, Kyiv, Ukraine; Reva et al, 2004), and Bacillus pumilus INR7 (J. Kloepper, Auburn University, AL, USA; Raupach & Kloepper, 1998). Bacteria were cultivated routinely in Luria broth (LB) solidified with 1.5 % agar.…”

Section: Methodsmentioning

confidence: 99%

Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7T and FZB42T: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on complete genome sequence comparisons

Borriss

Chen

Rueckert

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

253

178

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The whole-genome-sequenced rhizobacterium Bacillus amyloliquefaciens FZB42T (Chen et al., 2007) and other plant-associated strains of the genus Bacillus described as belonging to the species Bacillus amyloliquefaciens or Bacillus subtilis are used commercially to promote the growth and improve the health of crop plants. Previous investigations revealed that a group of strains represented a distinct ecotype related to B. amyloliquefaciens; however, the exact taxonomic position of this group remains elusive (Reva et al., 2004). In the present study, we demonstrated the ability of a group of Bacillus strains closely related to strain FZB42T to colonize Arabidopsis roots. On the basis of their phenotypic traits, the strains were similar to Bacillus amyloliquefaciens DSM 7T but differed considerably from this type strain in the DNA sequences of genes encoding 16S rRNA, gyrase subunit A (gyrA) and histidine kinase (cheA). Phylogenetic analysis performed with partial 16S rRNA, gyrA and cheA gene sequences revealed that the plant-associated strains of the genus Bacillus, including strain FZB42T, formed a lineage, which could be distinguished from the cluster of strains closely related to B. amyloliquefaciens DSM 7T. DNA–DNA hybridizations (DDH) performed with genomic DNA from strains DSM 7T and FZB42T yielded relatedness values of 63.7–71.2 %. Several methods of genomic analysis, such as direct whole-genome comparison, digital DDH and microarray-based comparative genomichybridization (M-CGH) were used as complementary tests. The group of plant-associated strains could be distinguished from strain DSM 7T and the type strain of B. subtilis by differences in the potential to synthesize non-ribosomal lipopeptides and polyketides. Based on the differences found in the marker gene sequences and the whole genomes of these strains, we propose two novel subspecies, designated B. amyloliquefaciens subsp. plantarum subsp. nov., with the type strain FZB42T ( = DSM 23117T = BGSC 10A6T), and B. amyloliquefaciens subsp. amyloliquefaciens subsp. nov., with the type strain DSM 7T( = ATCC 23350T = Fukumoto Strain FT), for plant-associated and non-plant-associated representatives, respecitvely. This is in agreement with results of DDH and M-CGH tests and the MALDI-TOF MS of cellular components, all of which suggested that the ecovars represent two different subspecies.

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Section: Methodsmentioning

confidence: 99%

Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7T and FZB42T: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on complete genome sequence comparisons

Borriss

Chen

Rueckert

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

253

178

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“…Several Bacillus-based commercial products are available, such as Quantum-400 (B. subtilis GB03), Serenade (B. subtilis QST713), and Rhizovital (B. amyloliquefaciens FZB42) (20,21). Bacillus spp.…”

mentioning

confidence: 99%

Bacilysin from Bacillus amyloliquefaciens FZB42 Has Specific Bactericidal Activity against Harmful Algal Bloom Species

Chen

et al. 2014

Appl Environ Microbiol

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bHarmful algal blooms, caused by massive and exceptional overgrowth of microalgae and cyanobacteria, are a serious environmental problem worldwide. In the present study, we looked for Bacillus strains with sufficiently strong anticyanobacterial activity to be used as biocontrol agents. Among 24 strains, Bacillus amyloliquefaciens FZB42 showed the strongest bactericidal activity against Microcystis aeruginosa, with a kill rate of 98.78%. The synthesis of the anticyanobacterial substance did not depend on Sfp, an enzyme that catalyzes a necessary processing step in the nonribosomal synthesis of lipopeptides and polyketides, but was associated with the aro gene cluster that is involved in the synthesis of the sfp-independent antibiotic bacilysin. Disruption of bacB, the gene in the cluster responsible for synthesizing bacilysin, or supplementation with the antagonist N-acetylglucosamine abolished the inhibitory effect, but this was restored when bacilysin synthesis was complemented. Bacilysin caused apparent changes in the algal cell wall and cell organelle membranes, and this resulted in cell lysis. Meanwhile, there was downregulated expression of glmS, psbA1, mcyB, and ftsZ-genes involved in peptidoglycan synthesis, photosynthesis, microcystin synthesis, and cell division, respectively. In addition, bacilysin suppressed the growth of other harmful algal species. In summary, bacilysin produced by B. amyloliquefaciens FZB42 has anticyanobacterial activity and thus could be developed as a biocontrol agent to mitigate the effects of harmful algal blooms. Eutrophication of surface waters has many undesirable effects and can lead to major water quality issues in freshwater and coastal systems (1). This phenomenon results in blooms of harmful algal species in freshwater lakes and brackish waters throughout the world. Moreover, the excessive growth of harmful algae, such as microalgae and cyanobacteria, often increases the production of inherent toxins such as microcystins and nodularins that cause acute poisonings of fish, birds, and mammals, including humans (2). For example, dogs died after they were exposed to a cyanobacterial bloom of Microcystis aeruginosa in Lake Amstelmeer (The Netherlands), and the concentration of microcystin in this lake was up to 5.27 ϫ 10 3 g g Ϫ1 of dry weight (3). In recent years, harmful algal blooms of eutrophic water in China have occurred frequently, including in Lake Taihu and Lake Chaohu, and Microcystis is thought to be the dominant bloom genera (4).Many control techniques have been used to prevent and mitigate bloom problems, including yellow loess (5), clay (6), and chemical agents such as copper sulfate and hydrogen peroxide (7). However, each of the physical and chemical methods available to remediate eutrophic water is associated with certain disadvantages (8). Therefore, there is still a pressing need for environmentally friendly, cost-effective, and convenient bactericidal agents directed against cyanobacterial blooms in eutrophic lakes. Biological control agents such as bact...

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“…Several strains of B. subtilis have been reported to be capable of forming multicellular structures or biofilms in rhizosphere and roots to enhance the plant growth (Bais et al 2004). The commercial B. subtilis fertilizers (Kodiak, Quantum-400 and Serenade) are successfully employed in the agricultural field to enhance the crop yield (Brannen and Kenney 1997;Ngugi et al 2005;Cawoy et al 2011). The bio-control aspects of B. subtilis are studied against few plant pathogens, but there is a lack of understanding the mitigation strategy of B. subtilis in crop plants infected with M. phaseolina.…”

Section: Introductionmentioning

confidence: 99%

Plant defense approach ofBacillus subtilis(BERA 71) againstMacrophomina phaseolina(Tassi) Goid in mung bean

Hashem

Radhakrishnan

Kumar

2017

Journal of Plant Interactions

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This study was aimed to elucidate the mitigation mechanism of an endophytic bacterium, Bacillus subtilis (BERA 71) against Macrophomina phaseolina (Tassi) Goid disease in mung bean. M. phaseolina reduced the plant growth by inducing disease, hydrogen peroxide (H 2 O 2 ) and lipid peroxidation. The inoculation of B. subtilis to diseased plants increased chlorophyll, ascorbic acids, and superoxide dismutase, catalase, peroxidase, ascorbate peroxidase and glutathione reductase activities, and while inhibited H 2 O 2 and lipid peroxidation for enhancing plant growth. In addition, B. subtilis association in plants mitigated the M. phaseolina infection due to increase of indole acetic acids and indole butyric acid, and also a decrease of abscisic acid. However, the nutrients (N, K, Ca, Mg, Zn, Cu, Mn and Fe) were increased, except Na in M. phaseolina diseased plants treated with B. subtilis. The result of this study suggests that B. subtilis interaction with plants can modulate the metabolism of pigments, hormones, antioxidants and nutrients against M. phaseolina to induce disease resistance in mung bean. ARTICLE HISTORY

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Kodiak � --a successful biological-control product for suppression of soil-borne plant pathogens of cotton

Cited by 121 publications

References 1 publication

Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7T and FZB42T: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on complete genome sequence comparisons

Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7T and FZB42T: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on complete genome sequence comparisons

Bacilysin from Bacillus amyloliquefaciens FZB42 Has Specific Bactericidal Activity against Harmful Algal Bloom Species

Plant defense approach ofBacillus subtilis(BERA 71) againstMacrophomina phaseolina(Tassi) Goid in mung bean

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