Knockdown of SERPINE1 reverses resistance of triple‑negative breast cancer to paclitaxel via suppression of VEGFA

Zhang, Qian; Li, Lei; Jiang, Di

doi:10.3892/or.2020.7770

Cited by 39 publications

(45 citation statements)

References 59 publications

(65 reference statements)

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“…In estrogen receptor (ER)-negative breast cancer, it has been indicated that ATF4 can activate the expression of the oncogene PSAT1 and promote cell proliferation [ 40 ]. Knockdown of SERPINE1 was found to significantly inhibit cell survival and induce apoptosis of breast cancer cells in vitro [ 41 ]. SESN2 is involved in the suppression of genesis of breast cancer genesis through its interaction with AMP-activated protein kinase (AMPK) [ 42 ].…”

Section: Resultsmentioning

confidence: 99%

Transcriptome study of oleanolic acid in the inhibition of breast tumor growth based on high-throughput sequencing

Liang

Pan

Xia

et al. 2021

Aging

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The function of oleanolic acid (OA) in various types of cancer has been reported frequently, especially for breast cancer. However, the regulation of breast tumor growth in response to OA treatment has not been studied in depth. Here, we first explored the effect of OA treatment on breast tumors in vitro and in vivo and then used RNA-seq technology to study the effect and molecular mechanism of OA treatment of MCF-7 cells, particularly at the level of functional genomics. The results showed that 40 μM OA treatment could significantly inhibit the proliferation and induce the apoptosis of MCF-7 cells. Through analysis of RNA sequencing data quality and differentially expressed genes (DEGs), 67 significantly downregulated genes and 260 significantly upregulated genes were identified to be involved in OA treatment of MCF-7 cells. Among these genes, 43 unique DEGs were enriched in several signaling pathways and Gene Ontology terms, such as p53 signaling pathway, TNF signaling pathway and mTOR signaling pathway. Six downregulated genes, including THBS1, EDN1, CACNG4, CCN2, AXIN2 and BMP4, as well as six upregulated genes, including ATF4, SERPINE1, SESN2, PPARGC1A, EGR1 and JAG1, were selected as target genes in response to OA treatment. The inhibitory effect of OA on breast cancer was also found in the following mouse experiments. Our study provides evidence and molecular support for the treatment of breast cancer with OA.

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Section: Resultsmentioning

confidence: 99%

Transcriptome study of oleanolic acid in the inhibition of breast tumor growth based on high-throughput sequencing

Liang

Pan

Xia

et al. 2021

Aging

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“…SERPINE1 may have a role in the establishment of the disease. Indeed, SERPINE1 has been reported to be involved in angiogenesis, observed as enriched in MF tissues compared to M, and tumorigenesis [ 47 ]. Based on this, SERPINE1 expression in the myometrium could be used for early diagnosis of the disease and as a therapeutic target.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analyses of Myometrium from Fibroid Patients Reveals Phenotypic Differences Compared to Non-Diseased Myometrium

Paul

Burns

Carpenter

et al. 2021

IJMS

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Uterine fibroid tissues are often compared to their matched myometrium in an effort to understand their pathophysiology, but it is not clear whether the myometria of uterine fibroid patients represent truly non-disease control tissues. We analyzed the transcriptomes of myometrial samples from non-fibroid patients (M) and compared them with fibroid (F) and matched myometrial (MF) samples to determine whether there is a phenotypic difference between fibroid and non-fibroid myometria. Multidimensional scaling plots revealed that M samples clustered separately from both MF and F samples. A total of 1169 differentially expressed genes (DEGs) (false discovery rate < 0.05) were observed in the MF comparison with M. Overrepresented Gene Ontology terms showed a high concordance of upregulated gene sets in MF compared to M, particularly extracellular matrix and structure organization. Gene set enrichment analyses showed that the leading-edge genes from the TGFβ signaling and inflammatory response gene sets were significantly enriched in MF. Overall comparison of the three tissues by three-dimensional principal component analyses showed that M, MF, and F samples clustered separately from each other and that a total of 732 DEGs from F vs. M were not found in the F vs. MF, which are likely understudied in the pathogenesis of uterine fibroids and could be key genes for future investigation. These results suggest that the transcriptome of fibroid-associated myometrium is different from that of non-diseased myometrium and that fibroid studies should consider using both matched myometrium and non-diseased myometrium as controls.

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“…SERPINE1 is a fibrinolytic inhibitor that has been involved in several human tumor malignancies. For example, SERPINE1 has been indicated to be associated with PTX resistance of breast cancer [ 31 ], and LncRNA LINC00200 promotes the progression of gastric cancer by regulating miR-143-3p/SERPINE1 axis [ 32 ]. Li et, al have reported that CEP72 induces bladder urothelial carcinoma cell aggressiveness by overexpression of SERPINE1 [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of a novel circular RNA circZNF652/miR-486-5p/SERPINE1 signaling cascade that regulates cancer aggressiveness in glioblastoma (GBM)

et al. 2022

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Circular RNAs (circRNAs) are closely associated with cancer development in glioblastoma (GBM), and this study aims to explore the molecular mechanisms of a novel circular RNA circZNF652 in regulating GBM aggressiveness. The present study found that CircZNF652 and SERPINE1 were upregulated, while miR-486-5p was downregulated in GBM tissues and cell lines, and GBM patients with high expression of CircZNF652 and SERPINE1, and patients with low expression of miR-486-5p tended to have a worse prognosis. Further results validated that both silencing of circZNF652 and miR-486-5p overexpression suppressed cell growth, migration, invasion, epithelial–mesenchymal transition (EMT) and tumorigenesis in GBM cells in vitro and in vivo . Next, the underlying mechanisms were investigated, and we found that circZNF652 sponged miR-486-5p to upregulate SERPINE1 in GBM cells. Also, we validated that knock-down of circZNF652 regulated the miR-486-5p/SERPINE1 axis to reverse the malignant phenotypes in GBM cells. Interestingly, we noticed that GBM cells derived exosomes were characterized by high-expressed CircZNF652. Collectively, we concluded that targeting the circular RNA circZNF652/miR-486-5p/SERPINE1 axis was a novel and effective strategy to suppress cancer progression in GBM.

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Knockdown of SERPINE1 reverses resistance of triple‑negative breast cancer to paclitaxel via suppression of VEGFA

Cited by 39 publications

References 59 publications

Transcriptome study of oleanolic acid in the inhibition of breast tumor growth based on high-throughput sequencing

Transcriptome study of oleanolic acid in the inhibition of breast tumor growth based on high-throughput sequencing

Transcriptome Analyses of Myometrium from Fibroid Patients Reveals Phenotypic Differences Compared to Non-Diseased Myometrium

Identification of a novel circular RNA circZNF652/miR-486-5p/SERPINE1 signaling cascade that regulates cancer aggressiveness in glioblastoma (GBM)

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