Klebsiella serotype-13 capsular polysaccharide: Primary structure and depolymerization by a bacteriophage-borne glycanase

Niemann, Heiner; Frank, Norbert; Stirm, Stephan

doi:10.1016/s0008-6215(00)83303-0

Cited by 47 publications

(19 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In comparison with the previously published pentasaccharide structure, [23] oligosaccharide P1 had a different reducing end, as evidenced by the HMBC spectrum. The anomeric proton of residue A had no interresidual connectivity …”

Section: β-D-manp-(1 → 4)-α-d-glcpsupporting

confidence: 40%

See 1 more Smart Citation

Preparation and Characterization of the Enzymatic Degradation Products of the Exopolysaccharide FromKlebsiellaK13

Shang

Liu

et al. 2014

Journal of Carbohydrate Chemistry

View full text Add to dashboard Cite

Oligosaccharides were prepared from the exopolysaccharide of Klebsiella K13 by enzymatic degradation and characterized. A phage-borne depolymerase enzyme was used to degrade the exopolysaccharide of Klebsiella K13. Bio-Gel P4 and P6 were used to purify the oligosaccharide products. The purified oligosaccharides were characterized by HPLC, mass spectroscopy, infrared spectroscopy, and NMR spectroscopy. The monosaccharide constituents of these enzymatic degradation products include D-glucose, D-galactose, D-mannose, and D-glucuronic acid. It was concluded that a pentasaccharide repeating unit with the following structure, as well as its dimer and trimer, was released from the exopolysaccharide: 3,4-O-(1-carboxyethylidene)-β-D-Galp-(1→4)-α-DGlcpA-(1→3)-β-D-Manp-(1→4)-α-D-Glcp-(1→3)-D-Glcp

show abstract

Section: β-D-manp-(1 → 4)-α-d-glcpsupporting

confidence: 40%

“…Niemann [23] analyzed the structure of the EPS from Klebsiella K13 using bacteriophage degradation and proton NMR spectroscopy. The previously published structure of the repeating unit of EPS from Klebsiella K13 was:…”

Section: β-D-manp-(1 → 4)-α-d-glcpmentioning

confidence: 99%

Preparation and Characterization of the Enzymatic Degradation Products of the Exopolysaccharide FromKlebsiellaK13

Shang

Liu

et al. 2014

Journal of Carbohydrate Chemistry

View full text Add to dashboard Cite

show abstract

“…This can increase the head size rather than genome size. Other workers also reported isolation of the K. pneumoniae specific phages belonging to families Siphoviridae and Myoviridae (Niemann et al 1977;Wu et al 2007).…”

Section: Discussionmentioning

confidence: 92%

Isolation and characterization of Klebsiella pneumoniae specific bacteriophages from sewage samples

2010

View full text Add to dashboard Cite

Five bacteriophages (Kpn5, Kpn12, Kpn13, Kpn17 and Kpn22), each having specificity against Klebsiella pneumoniae strain B5055, were isolated from sewage samples and characterized in terms of growth characteristics, genetic material, morphology and structural proteins. Adsorption rate as well as single step growth curve experiments showed variation among phages. Restriction enzyme digestion of DNA confirmed the presence of double stranded DNA as well as the heterogeneous nature of genetic material. RAPD-PCR was performed to further distinguish these closely related phages. Their genome fingerprint confirmed their diversity. Transmission electron microscopy, on the other hand, showed their morphological similarity; they were assigned to family Podoviridae, order Caudovirales on the basis of their head and tail morphology. Structural proteins resolved on SDS-PAGE showed the presence of similar major outer membrane proteins. The bacteriophages, belonging to Podoviridae family with short stumpy tails, were found to be nontoxic to mice. They showed maximum count in various organs at 6 h post inoculation, which persisted till 36 h. These phages thus have the potential to be used for phage therapy.

show abstract

“…In addition, phages are strain-specific, highly efficient against biofilms, and easy to isolate and manipulate. Capsule depolymerases have multiple applications, including use as therapeutic agents against bacterial pathogens 37, 38 , for the prevention or eradication of biofilms 39, 40 , and for the production of oligosaccharides from polysaccharides 39–41 . Our previous study has indicated that a bacteriophage (NTUH-K2044-K1-1) and its capsule depolymerase exhibit specificity for encapsulated K .…”

Section: Introductionmentioning

confidence: 99%

Two T7-like Bacteriophages, K5-2 and K5-4, Each Encodes Two Capsule Depolymerases: Isolation and Functional Characterization

Hsieh

Lin

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Two Klebsiella bacteriophages K5-2 and K5-4, which are able to infect and grow on either capsular types K30/K69 and K5 or K8 and K5 of Klebsiella strains, were isolated and characterized. Each phage contained two open reading frames (ORFs), which encoded two putative capsule depolymerases, respectively. The first ORF encoded tail fiber proteins, which have K30/K69 depolymerase and K8 depolymerase activities. The second ORF encoded hypothetical proteins, which are almost identical in amino acid sequences, and have K5 depolymerase activity. Alcian blue staining of enzyme-treated capsular polysaccharides (CPS) showed that purified depolymerases can cleave purified Klebsiella CPS in vitro and liberate monosaccharaides. Capsule K5 deletion mutants were not lysed by either phage, suggesting that the capsule was essential for phage infection. Bacterial killing was observed when incubated Klebsiella strains with phages but not with purified depolymerases. Treatment with the K5-4 phage significantly increased the survival of mice infected with a K. pneumoniae K5 strain. In conclusion, two dual host-specific Klebsiella phages and their tailspikes exhibit capsule depolymerase activity were characterized. Each phage and phage-encoded depolymerase has specificity for capsular type K30/K69, K8 or K5, and could be used for the typing and treatment of K. pneumoniae infection.

show abstract

Klebsiella serotype-13 capsular polysaccharide: Primary structure and depolymerization by a bacteriophage-borne glycanase

Cited by 47 publications

References 45 publications

Preparation and Characterization of the Enzymatic Degradation Products of the Exopolysaccharide FromKlebsiellaK13

Preparation and Characterization of the Enzymatic Degradation Products of the Exopolysaccharide FromKlebsiellaK13

Isolation and characterization of Klebsiella pneumoniae specific bacteriophages from sewage samples

Two T7-like Bacteriophages, K5-2 and K5-4, Each Encodes Two Capsule Depolymerases: Isolation and Functional Characterization

Contact Info

Product

Resources

About