Klebsiella Pneumoniaeoxa-48 in a Urology Patient: Case Report

Jajić, Ines; Benčić, Ana; Siroglavić, Marko; Zarfel, Gernot; Ružić, Boris; Pezelj, Ivan; Bedenić, Branka

doi:10.20471/acc.2017.56.01.23

Cited by 4 publications

(4 citation statements)

References 30 publications

(34 reference statements)

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“…Therefore, detection of MBLs in A. baumannii is a huge challenge to clinical microbiologists, particularly in isolates with multiple carbapenem-resistance mechanisms, such as those co-harboring CHDL and MBL. The difficulties with phenotypic detection have also been reported for OXA-48 β-lactamase in Enterobacteriaceae ( 29 ).…”

Section: Discussionmentioning

confidence: 86%

False Positive Phenotypic Detection of Metallo-Beta-Lactamases in Acinetobacter Baumannii

Bedenić

Ladavac

Vranić-Ladavac³

et al. 2019

ACC

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SUMMARY Phenotypic detection of metallo-β-lactamases (MBLs) in Acinetobacter (A.) baumannii is a serious challenge to clinical microbiologists. MBLs are inhibited by metal chelators such as ethylenediaminetetraacetic acid) (EDTA). Production of MBLs cannot be recognized based on resistance phenotype. Therefore, phenotypic tests using EDTA are recommended. The aim of this study was to investigate the sensitivity and specificity of inhibitor based tests (EDTA) for detection of MBL. A total of 172 A. baumannii strains (123 carbapenemase positive and 49 carbapenemase negative) were analyzed. Phenotypic detection of MBLs was performed by the combined disk test with EDTA (CDT-EDTA) and EPI-dilution test (EPI-DT). Both tests were positive in all 11 isolates possessing VIM-1 MBL, showing 100% sensitivity. However, false positive results were observed in strains with class D carbapenemases using both tests, i.e. all OXA-23 and OXA-24/40 producing organisms and most OXA-58 positive strains (77% with CDT-EDTA vs . 65% with EPI-DT). False positive results can occur because oxacillinases are converted to a less active state in the presence of EDTA, leading to augmentation of the inhibition zone around the carbapenem disk or reduction of carbapenem minimum inhibitory concentrations. This study showed high sensitivity but low specificity of phenotypic methods in the detection of MBLs.

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Section: Discussionmentioning

confidence: 86%

False Positive Phenotypic Detection of Metallo-Beta-Lactamases in Acinetobacter Baumannii

Bedenić

Ladavac

Vranić-Ladavac³

et al. 2019

ACC

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“…Dentro dos aminoglicosídeos, a amicacina apresentou menor taxa de resistência e é a que tem menor potencial para induzir a resistência bacteriana, pois a K. pneumoniae possui pouca detecção do gene metilase, o principal mecanismo de resistência a esse fármaco. Por isso, são muito utilizados para o tratamento de infecções nosocomiais (Jajić et al, 2017;Zheng et al, 2018).…”

Section: Discussionunclassified

“…São utilizados em animais de companhia, em infecções graves (Monteani-Ferreira et al, 1999). Já os isolados de humanos têm apresentado alta taxa de resistência pela presença enzima carbapenemase (KPC), como descrito pelos autores (Pereira et al, 2015;Jajić et al, 2017).…”

Section: Discussionunclassified

Perfil de resistência antimicrobiana de Klebsiella pneumoniae isoladas de animais domésticos e silvestres

Sousa

Makino

Bruno

et al. 2019

Arq. Bras. Med. Vet. Zootec.

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RESUMO Klebsiella pneumoniae é um patógeno oportunista, responsável por diversos tipos de infecções nosocomiais, e é considerado um microrganismo multirresistente. Dados na literatura que forneçam informações a respeito da resistência desse microrganismo a antimicrobianos em amostras de animais são escassos. Dessa forma, o objetivo deste trabalho foi avaliar o perfil e o seu aumento das resistências a antimicrobianos dentro da medicina veterinária. Um total de 67 isolados de K. pneumoniae, provenientes de diferentes sítios de isolamento de animais domésticos (39/67) e silvestres (28/67), foi confirmado por sequenciamento do gene 16S rRNA. O maior percentual de isolamento de K. pneumoniae foi de amostras de urina, com 16% (11/67), fezes, com 15% (10/67), e pulmão, com 13,5% (09/67). No perfil de resistência, foram testadas 11 categorias de antibióticos, sendo a maior taxa de resistência ao metronidazol 97% (65/67), à ampicilina 94% (63/67), à amoxicilina 93% (62/67), às sulfonamidas 93% (62/67), à colistina 93% (62/67) e à nitrofurantoína 88% (59/67). Aqueles que apresentaram menor taxa de resistência foram: meropenem 3% (2/67), imipenem 6% (4/67) e amicacina 16% (11/67). Todos os isolados foram considerados bactérias multirresistentes (MRD), com o índice de resistência múltipla aos antibióticos (IRMA) variando de 0,15 a 0,85 e com 60 tipos de padrões de resistência. O resultado deste estudo reforça que os animais são reservatórios de K. pneumoniae multirresistentes.

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“…We hypothesize that resistance to imipenem has been acquired from an environment contaminated with resistant pathogens and exchange of resistance genes since the use of carbapenems in animals is still limited to treating serious diseases in companion animals [29,45]. As for the isolates of human origin, they have shown a high rate of resistance due to the presence of the KPC enzyme, as previously described [46].…”

Section: Discussionmentioning

confidence: 73%

Determination of multidrug-resistant populations and molecular characterization of complex Klebsiella spp. in wild animals by multilocus sequence typing

et al. 2022

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Background and Aim: One of the most significant public health concerns is multidrug-resistant (MDR) microorganisms. Klebsiella spp. have been at the forefront of causing different types of infections such as bacteremia, urinary tract infections, pneumonia, enteritis, and sepsis in humans as well as animals. This study aimed to determine the genomic similarity between Klebsiella spp. isolated from wild animal samples and those described in the Institut Pasteur genomic database to verify the spread of resistant clones regionally in the state of Mato Grosso, and to compare the epidemiological data in different regions of Brazil and the world. Materials and Methods: Isolates from various sites of injury in wild animals were identified by sequencing the 16S rRNA gene. Antimicrobial susceptibility testing was performed using the disk diffusion method to verify the resistance profile, and then, multilocus sequence typing was performed to verify the population structure and compare the isolates from other regions of Brazil and the world. Results: Twenty-three sequence types (STs) were observed; of these, 11 were new STs, as new alleles were detected. There was no predominant ST among the isolates. All isolates were MDR, with high rates of resistance to sulfonamides, ampicillin, amoxicillin, and nitrofurantoin and low resistance to meropenem, imipenem, and amikacin. Conclusion: Improving our understanding of the population structure of Klebsiella spp. in wild animals may help determine the source of infection during outbreaks in humans or animals, as the One Health concept emphasizes the interlinks between humans, animals, and environmental health.

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Klebsiella Pneumoniaeoxa-48 in a Urology Patient: Case Report

Cited by 4 publications

References 30 publications

False Positive Phenotypic Detection of Metallo-Beta-Lactamases in Acinetobacter Baumannii

False Positive Phenotypic Detection of Metallo-Beta-Lactamases in Acinetobacter Baumannii

Perfil de resistência antimicrobiana de Klebsiella pneumoniae isoladas de animais domésticos e silvestres

Determination of multidrug-resistant populations and molecular characterization of complex Klebsiella spp. in wild animals by multilocus sequence typing

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