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Rao, Rifaqat Ali Khan; Ajmal, Mohammad; Siddiqui, Bilquees Ata; Ahmad, Sarfaraz

doi:10.1023/a:1005999418810

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Ultraviolet-visible Spectrophotometric Detection1

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2003

2021

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Cited by 4 publications

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“…A study was carried out on the performance of direct phase (silica gel) and RP (C 18 ) columns, using a MeOH-0.1 M NaClO 4 mobile phase, for the HPLC-UVD (at 256 nm) analysis of five aromatic amines: aniline (1a), 1-(8a) and 2-napththylamine (9a), di-(DPA) and triphenylamine (TPA). Good resolutions and separation factors were observed with the RP column for 1a vs. the other analytes and for 8a or 9a vs. DPA or TPA; however, separation of the 8a-9a or DPA-TPA pairs was poor 210 .…”

Section: Ultraviolet-visible Spectrophotometric Detectionmentioning

confidence: 94%

Analytical Aspects of Aromatic Amines

Zabicky

2009

Patai's Chemistry of Functional Groups

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Section: Ultraviolet-visible Spectrophotometric Detectionmentioning

confidence: 94%

Analytical Aspects of Aromatic Amines

Zabicky

2009

Patai's Chemistry of Functional Groups

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Rapid determination of amino acids in biological samples using a monolithic silica column

2011

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A high-performance liquid chromatography method in which fluorescence detection is used for the simultaneous determination of 21 amino acids is proposed. Amino acids were derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) and then separated on a monolithic silica column (MonoClad C18-HS, 150 mm×3 mm i.d.). A mixture of 25 mM citrate buffer containing 25 mM sodium perchlorate (pH 5.5) and acetonitrile was used as the mobile phase. We found that the most significant factor in the separation was temperature, and a linear temperature gradient from 30 to 49°C was used to control the column temperature. The limits of detection and quantification for all amino acids ranged from 3.2 to 57.2 fmol and 10.8 to 191 fmol, respectively. The calibration curves for the NBD-amino acid had good linearity within the range of 40 fmol to 40 pmol when 6-aminocaproic acid was used as an internal standard. Using only conventional instruments, the 21 amino acids could be analyzed within 10 min. This method was found to be suitable for the quantification of the contents of amino acids in mouse plasma and adrenal gland samples.

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