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Shubnikova, E. A.; Chentsov, Yu. S.

doi:10.1023/a:1026678829760

Cited by 4 publications

(8 citation statements)

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“…2). This vesicular release of secretion by the endocrine type as a sign of secretion intensifi cation had been described previously in diabetic animals treated with isoproteronol and in animals after pancreas removal [1,3]. Presumably, transplantation of SMG to animals with STZ diabetes led to intense production and extrusion of protein from the granular compartment cells.…”

Section: Resultssupporting

confidence: 68%

“…Diabetes is associated with morphological and functional changes, including changes in the expression of secretory proteins, accumulation of lipid droplets in secretory cells, reduced response to cholinergic stimulation, increase of autophagal and lysosomal activities, endocytosis of secretory proteins, and accumulation of membrane material in ductal cells of the salivary gland [1,3,7,12]. Our experiments on animals with STZ diabetes confi rmed the development of some of these changes.…”

Section: Resultsmentioning

confidence: 58%

“…Presumably, transplantation of SMG to animals with STZ diabetes led to intense production and extrusion of protein from the granular compartment cells. This insulin-like protein of extrapancreatic origin is suffi ciently well studied [3]. The effect of isoproteronol on protein production rhythm in normal animals and animals with alloxan diabetes has been described.…”

Section: Resultsmentioning

confidence: 99%

“…The search for new means providing stable compensation of diabetes mellitus and reducing the risk of secondary complications is an important problem of modern science. The use of salivary glands as the source of insulin-producing cells seems to be a promising approach: numerous experimental data demonstrated the presence of insulin-like protein in cells of the granular compartment of submandibular gland (SMG) ducts [3,10,11]. This protein is similar to pancreatic insulin by many characteristics in this animal species [3].…”

mentioning

confidence: 99%

“…SMG organogenesis can change under these conditions and its compensatory effects can manifest at an earlier age [2]. In addition, the compensatory function of the salivary gland has been found in depancreatized animals, in animals with induced diabetes mellitus, and animals treated with isoproteronol (nonselective β-adrenergic receptor agonist) [1,3]. It is noteworthy that isoproteronol treatment stimulates the synthesis and extrusion of insulin-like protein into the blood, which leads to reduction and even disappearance of diabetes symptoms in animals [1,3].…”

mentioning

confidence: 99%

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Compensatory function of submandibular gland in mice with streptozotocin diabetes under conditions of transplantation

2012

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Transplantation of the submandibular gland under the renal capsule of mice with streptozotocin-induced diabetes mellitus stimulated the compensatory function of the recipient submandibular gland. An increase in insulin I and insulin II gene expression in the submandibular gland after transplantation was demonstrated by PCR. More intensive production and extrusion of these proteins in the apical and basal directions in granular compartment cells of the submandibular gland was confirmed by electron microscopy. All these changes led to a reduction of blood glucose levels in diabetic animals as soon as 2-2.5 weeks after transplantation.

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Section: Resultssupporting

confidence: 68%

Section: Resultsmentioning

confidence: 58%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Compensatory function of submandibular gland in mice with streptozotocin diabetes under conditions of transplantation

2012

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Valproic Acid Increases the Hepatic Differentiation Potential of Salivary Gland Cells

et al. 2015

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The studies of cell plasticity and differentiation abilities are important problems in modern cellular biology. The use of histone deacetylase inhibitor - valproic acid is a promising approach to increasing the differentiation efficiency of various cell types. In this paper we investigate the ability of mouse submandibular salivary gland cells to differentiate into the hepatic direction and the effect of valproic acid on the efficiency of this differentiation. It was shown that the gene expression levels of hepatocyte markers (Aat, Afp, G6p, Pepck, Tat, Cyp3a13) and liver-enriched transcription factors (Hnf-3α, Hnf-3β, Hnf-4α, Hnf-6) were increased after differentiation in salivary gland cells. Valproic acid increases the specificity of hepatic differentiation, reducing the expression levels of the ductal (Krt19, Hhex1, Cyp7a1) and acinar (Ptf1a) markers. After valproic acid exposure, the efficiency of hepatic differentiation also increases, as evidenced by the increase in the gene expression level of Alb and Tdo, and increase in urea production by differentiated cells. No change was found in DNA methylation of the promoter regions of the genes; however, valproic acid treatment and subsequent hepatic differentiation largely affected the histone H3 methylation of liver-enriched genes. Thus, mouse submandibular salivary gland cells are capable of effective differentiation in the hepatic direction. Valproic acid increases the specificity and efficiency of the hepatic differentiation of these cells.

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Stem Cells in the Treatment of InsulinDependent Diabetes Mellitus

et al. 2016

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Diabetes affects over 350 million people worldwide, with the figure projected to rise to nearly 500 million over the next 20 years, according to the World Health Organization. Insulin-dependent diabetes mellitus (type 1 diabetes) is an endocrine disorder caused by an autoimmune reaction that destroys insulin-producing -cells in the pancreas, which leads to insulin deficiency. Administration of exogenous insulin remains at the moment the treatment mainstay. This approach helps to regulate blood glucose levels and significantly increases the life expectancy of patients. However, type 1 diabetes is accompanied by long-term complications associated with the systemic nature of the disease and metabolic abnormalities having a profound impact on health. Of greater impact would be a therapeutic approach which would overcome these limitations by better control of blood glucose levels and prevention of acute and chronic complications. The current efforts in the field of regenerative medicine are aimed at finding such an approach. In this review, we discuss the time-honored technique of donor islets of Langerhans transplantation. We also focus on the use of pluripotent stem and committed cells and cellular reprogramming. The molecular mechanisms of pancreatic differentiation are highlighted. Much attention is devoted to the methods of grafts delivery and to the materials used during its creation.

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Cited by 4 publications

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Compensatory function of submandibular gland in mice with streptozotocin diabetes under conditions of transplantation

Compensatory function of submandibular gland in mice with streptozotocin diabetes under conditions of transplantation

Valproic Acid Increases the Hepatic Differentiation Potential of Salivary Gland Cells

Stem Cells in the Treatment of InsulinDependent Diabetes Mellitus

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