Kinetics of urinary recovery of five sugars after orogastric administration in healthy dogs

Steiner, Jörg M.; Williams, David A.; Moeller, Erik M.

doi:10.2460/ajvr.2002.63.845

Cited by 14 publications

(14 citation statements)

References 17 publications

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“…The later was also observed in our study during meloxicam and meloxicam-misoprostol treatment at different time points. Hypertonic sugar solutions that were used in the present study may also cause osmotic diarrhoea, distended abdomen and flatulence (Uil et al, 1997;Uil et al, 2000;Steiner et al, 2002), which were observed in four dogs soon after sugar solutions were ingested. It is known that NSAIDs can affect gastrointestinal mucosa either by a local topical effect or via systemic actions.…”

Section: Resultsmentioning

confidence: 88%

Effect of meloxicam and meloxicam with misoprostol on serum prostaglandins and gastrointestinal permeability in healthy beagle dogs

Roškar¹,

Nemec²,

Jerin³

et al. 2011

Acta vet. (Beogr.)

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The aim of the present study was to investigate the effect of meloxicam and meloxicam with misoprostol on prostaglandin E2 (PGE2) and prostaglandin I2 (PGI2) serum concentration, as well as on gastrointestinal permeability. NSAIDs, such as meloxicam, have gastrointestinal side effects, which are due to prostaglandins depletion and topical damage. Seven adult beagle dogs were included in the study. Three different 20 days long treatments were carried out (placebo, meloxicam and meloxicam with misoprostol). The same seven dogs participated in all three treatments. On days 1 to 10 the dogs received placebo, meloxicam or meloxicam together with misoprostol PO. Dogs were than monitored from day 11 to 20. Samples for serum PGE2 and PGI2 concentration and plasma lactulose, mannitol and sucrose concentration determination were drawn on day 0, 2, 6, 11 and 20. Lactulose/mannitol (L/M) index was calculated. Treatment with meloxicam and meloxicam with misoprostol resulted in lower PGE2 and PGI2 serum concentrations in comparison to the placebo. L/M index and sucrose plasma concentration were increased in both groups in comparison to the placebo. According to the results of the study, meloxicam has altered gastrointestinal permeability and depleted prostaglandins production. Misoprostol was shown to be an effective preventing treatment

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Section: Resultsmentioning

confidence: 88%

Effect of meloxicam and meloxicam with misoprostol on serum prostaglandins and gastrointestinal permeability in healthy beagle dogs

Roškar¹,

Nemec²,

Jerin³

et al. 2011

Acta vet. (Beogr.)

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“…Other tests use different sugars such as lactulose, rhamnose, and sucrose. 17,18 Because of the complicated assays required to quantify sugars in serum, sugar permeability tests are usually performed in urine. 19 This requires urinary bladder catheterization of dogs, which is difficult, especially in bitches, and carries a risk of infection.…”

Section: Introductionmentioning

confidence: 99%

Estimation of intestinal permeability in healthy dogs using the contrast medium iohexol

Klenner¹,

Coenen

Failing

et al. 2009

Veterinary Clinical Pathol

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“…Sucrose has been determined and quantified in biological samples by several methods, including enzymatic assays, 14,33 high-performance liquid chromatography (HPLC)-mass spectrometry, 6,7,13 HPLC pulsed amperometric detector, 26 HPLC fluorescence detector, 15 and gas chromatography with mass spectrometry. 24 Gas chromatography with flame ionization detection (GC-FID) has been used for quantifying sucrose in urine 1,9 ; however, to the authors' knowledge, a GC-FID method for quantifying sucrose in serum has not been published.…”

Section: Research-article2014mentioning

confidence: 99%

Development and validation of a gas chromatography–flame ionization detection method for quantifying sucrose in equine serum

et al. 2014

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Abstract.A simple and accurate method for quantifying sucrose in equine serum that can be applied to sucrose permeability testing in the horse was developed and validated using gas chromatography with flame ionization detection. The assay provided an acceptable degree of linearity, accuracy, and precision at concentrations of sucrose as low as 2.34 μmol/l and as high as 20.45 μmol/l. Percentage recovery of sucrose from serum ranged from 89% to 102%; repeatability and intermediate precision (relative standard deviation) ranged from 3.6% to 6.7% and 4.1% to 9.3%, respectively. The limit of detection was 0.73 μmol/l. No interfering peaks were observed except lactose, which gave 2 peaks, one of which overlapped partially with sucrose. To evaluate the suitability of the method for quantifying sucrose in serum samples from horses with naturally occurring gastric ulceration, 10 horses with and without naturally occurring gastric ulceration were subjected to sucrose permeability testing. All horses demonstrated an increase in serum sucrose concentration over time following oral administration of sucrose; however, the increase from baseline was significant for horses with gastric ulceration at 45 min (P = 0.0082) and 90 min (P = 0.0082) when compared with healthy horses. It was concluded that gas chromatography with flame ionization detection is a valid method for quantifying sucrose in equine serum and can be applied directly to the analysis of sucrose in equine serum as part of a larger validation study aimed at developing a blood test for the diagnosis of gastric ulcers in horses.

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Kinetics of urinary recovery of five sugars after orogastric administration in healthy dogs

Abstract: In healthy dogs, a urine collection period of 6 hours is sufficient for gastrointestinal permeability and mucosal function testing following orogastric administration of lactulose, rhamnose, xylose, methylglucose, and sucrose.

Cited by 14 publications

References 17 publications

Effect of meloxicam and meloxicam with misoprostol on serum prostaglandins and gastrointestinal permeability in healthy beagle dogs

Effect of meloxicam and meloxicam with misoprostol on serum prostaglandins and gastrointestinal permeability in healthy beagle dogs

Estimation of intestinal permeability in healthy dogs using the contrast medium iohexol

Development and validation of a gas chromatography–flame ionization detection method for quantifying sucrose in equine serum

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