Kinetics of polyol oxidation with Gluconobacter oxydans

Ohrem, H. Leonhard; Voβ, Harald

doi:10.1007/bf00128385

Cited by 8 publications

(4 citation statements)

References 9 publications

(3 reference statements)

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“…The inoculum for the bioreactor originated from a shake-flask culture using sorbitol as substrate. According to previous works [10,11], the product DHA could not be metabolized by Gluconobacter oxydans as long as the substrate glycerol was present in the fermentation broth. Productivity was high for the pH range 4.5-6.0.…”

Section: Methodsmentioning

confidence: 86%

Optimization of the microbial synthesis of dihydroxyacetone from glycerol with Gluconobacter oxydans

Hekmat¹,

Bauer²,

Fricke

2003

Bioprocess and Biosystems Engineering

147

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An optimized repeated-fed-batch fermentation process for the synthesis of dihydroxyacetone (DHA) from glycerol utilizing Gluconobacter oxydans is presented. Cleaning, sterilization, and inoculation procedures could be reduced significantly compared to the conventional fed-batch process. A stringent requirement was that the product concentration was kept below a critical threshold level at all times in order to avoid irreversible product inhibition of the cells. On the basis of experimentally validated model calculations, a threshold value of about 60 kg x m(-3) DHA was obtained. The innovative bioreactor system consisted of a stirred tank reactor combined with a packed trickle-bed column. In the packed column, active cells could be retained by in situ immobilization on a hydrophilized Ralu-ring carrier material. Within 17 days, the productivity of the process could be increased by 75% to about 2.8 kg x m(-3) h(-1). However, it was observed that the maximum achievable productivity had not been reached yet.

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Section: Methodsmentioning

confidence: 86%

Optimization of the microbial synthesis of dihydroxyacetone from glycerol with Gluconobacter oxydans

Hekmat¹,

Bauer²,

Fricke

2003

Bioprocess and Biosystems Engineering

147

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“…Similar product (or feedback) inhibition can be expected with the sorbitol bioconversion process (Ohrem and Voss, 1995b).…”

Section: Product Inhibitionmentioning

confidence: 82%

“…The dehydrogenase enzymes provide only enough energy for maintenance and no growth is possible. Growth and respiratory activity are restored after supply of a nutrient, for example, yeast extract (Ohrem and Voss, 1995b). Critical Reviews in Biotechnology Downloaded from informahealthcare.com by Boston College on 09/16/13…”

Section: A Nutritional Requirementsmentioning

confidence: 99%

“…Carbon dioxide formation at low pH values is inhibited but remains relatively constant as long as the pH does not drop below 6.5. The enzymes of the pentose phosphate pathway that optimally operate at pH 7.0 are inactivated at pH 4.5 (Ohrem and Voss, 1995b). As almost all carbon dioxide produced arises via the pentose phosphate pathway, a lower pH (<4.5) would be advantageous for product formation, although nutrient requirements are increased at low pH values (Olijve and Kok, 1979).…”

Section: Phmentioning

confidence: 99%

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The GenusGluconobacterand Its Applications in Biotechnology

Macauley

McNeil

Harvey

2001

Critical Reviews in Biotechnology

104

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Organisms of the genus Gluconobacter have been widely utilized within the biotechnology industry for many decades, due to their unique metabolic characteristics. The metabolic features that render Gluconobacter so useful in biotransformation processes, vitamin synthesis, and, as the biological element in sensor systems, are critically evaluated, and the relevance of recent biochemical genetic studies to current and future industrial Gluconobacter processes is discussed. The impact of recombinant gene technology on the status of Gluconobacter processes and the potential use of such techniques in clarifying aspects of the physiology of Gluconobacter is reviewed.

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Entwicklung und Optimierung eines halbtechnischen semikontinuierlichen, zweistufigen Zulaufverfahrens zur mikrobiellen Herstellung von Dihydroxyaceton

Hekmat

Bauer

2006

Chemie Ingenieur Technik

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In manchen Fällen ist die Synthese von Feinchemikalien auf biologischem Wege wirtschaftlicher als über chemische Verfahren. Ein klassisches Beispiel hierfür ist die mikrobielle Herstellung von Dihydroxyaceton. Der konventionelle etablierte Prozess hierbei ist das diskontinuierliche Zulaufverfahren im Rührkesselbioreaktor. Im vorliegenden Beitrag wird ein innovatives semikontinuierliches, zyklisch betriebenes zweistufiges Zulaufverfahren im halbtechnischen Maßstab beschrieben, das gegenüber dem etablierten Prozess deutliche Vorteile hinsichtlich der Produktivität bringt. Eine Optimierung des Verfahrens wird durch die zyklische Zugabe einer zweiten Kohlenstoffquelle erzielt.

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Kinetics of polyol oxidation with Gluconobacter oxydans

Cited by 8 publications

References 9 publications

Optimization of the microbial synthesis of dihydroxyacetone from glycerol with Gluconobacter oxydans

Optimization of the microbial synthesis of dihydroxyacetone from glycerol with Gluconobacter oxydans

The GenusGluconobacterand Its Applications in Biotechnology

Entwicklung und Optimierung eines halbtechnischen semikontinuierlichen, zweistufigen Zulaufverfahrens zur mikrobiellen Herstellung von Dihydroxyaceton

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