The Genus<i>Gluconobacter</i>and Its Applications in Biotechnology

Macauley, Sue; McNeil, Brian; Harvey, Linda M.

doi:10.1080/20013891081665

Cited by 104 publications

(49 citation statements)

References 76 publications

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“…As a matter of fact, this enzyme plays a central role in the pentose phosphate pathway, which runs concomitantly with gluconacetan production (19,38). However, glucose-6-phosphate isomerase activity is higher on fructose than on the other substrates.…”

Section: Discussionmentioning

confidence: 99%

Influence of Nutritional Factors on the Nature, Yield, and Composition of Exopolysaccharides Produced by Gluconacetobacter xylinus I-2281

Kornmann

Duboc

Marison

et al. 2003

Appl Environ Microbiol

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The influence of substrate composition on the yield, nature, and composition of exopolysaccharides (EPS) produced by the food-grade strain Gluconacetobacter xylinus I-2281 was investigated during controlled cultivations on mixed substrates containing acetate and either glucose, sucrose, or fructose. Enzymatic activity analysis and acid hydrolysis revealed that two EPS, gluconacetan and levan, were produced by G. xylinus. In contrast to other acetic acid strains, no exocellulose formation has been measured. Considerable differences in metabolite yields have been observed with regard to the carbohydrate source. It was shown that glucose was inadequate for EPS production since most of this substrate (0.84 C-mol/C-mol) was oxidized into gluconic acid, 2-ketogluconic acid, and 5-ketogluconic acid. In contrast, sucrose and fructose supported a 0.35 C-mol/C-mol gluconacetan yield. In addition, growing G. xylinus on sucrose produced a 0.07 C-mol/C-mol levan yield. The composition of EPS remained unchanged during the course of the fermentations. Levan sucrase activity was found to be mainly membrane associated. In addition to levan production, an analysis of levan sucrase's activity also explained the formation of glucose oxides during fermentation on sucrose through the release of glucose. The biosynthetic pathway of gluconacetan synthesis has also been explored. Although the activity of key enzymes showed large differences to be a function of the carbon source, the ratio of their activities remained similar from one carbon source to another and corresponded to the ratio of precursor needs as deduced from the gluconacetan composition.

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Section: Discussionmentioning

confidence: 99%

Influence of Nutritional Factors on the Nature, Yield, and Composition of Exopolysaccharides Produced by Gluconacetobacter xylinus I-2281

Kornmann

Duboc

Marison

et al. 2003

Appl Environ Microbiol

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“…The bacteria are able to grow at a low pH of 3.5 in highly concentrated sugar solutions and on fruit such as apples, pears and grapes as well as in ciders (10,16,52). Gluconobacter species are found and utilized in fermentation processes and production of wine, vinegar, and vitamin C among others (10,36,55). To our knowledge, Gluconobacter asaii has not been previously associated with biocontrol activity.…”

mentioning

confidence: 96%

Biocontrol of the Food-Borne Pathogens Listeria monocytogenes and Salmonella enterica Serovar Poona on Fresh-Cut Apples with Naturally Occurring Bacterial and Yeast Antagonists

Leverentz

Conway

Janisiewicz

et al. 2006

Appl Environ Microbiol

123

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Fresh-cut apples contaminated with either Listeria monocytogenes or Salmonella enterica serovar Poona, using strains implicated in outbreaks, were treated with one of 17 antagonists originally selected for their ability to inhibit fungal postharvest decay on fruit. While most of the antagonists increased the growth of the food-borne pathogens, four of them, including Gluconobacter asaii (T1-D1), a Candida sp. (T4-E4), Discosphaerina fagi (ST1-C9), and Metschnikowia pulcherrima (T1-E2), proved effective in preventing the growth or survival of food-borne human pathogens on fresh-cut apple tissue. The contaminated apple tissue plugs were stored for up to 7 days at two different temperatures. The four antagonists survived or grew on the apple tissue at 10 or 25°C. These four antagonists reduced the Listeria monocytogenes populations and except for the Candida sp. (T4-E4), also reduced the S. enterica serovar Poona populations. The reduction was higher at 25°C than at 10°C, and the growth of the antagonists, as well as pathogens, increased at the higher temperature.

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“…Strains of this genus also produce aliphatic, aromatic carboxylic and thiocarboxylic acids that find applications as flavoring ingredients 6 . Furthermore, Gluconobacter enzymes and whole cells are thought to be ideal for use as the biological element in sensor systems for the detection of alcohols, sugars and polyols 7 . Natural habitats of G. oxydans are flowers and fruits 8 .…”

mentioning

confidence: 99%