Kinetics of Mx expression in rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar L.) parr in response to VHS–DNA vaccination

Acosta, F.; Petrie, Allan G.; Lockhart, K.; Lorenzen, Niels; Ellis, A.E.

doi:10.1016/j.fsi.2004.06.005

Cited by 38 publications

(25 citation statements)

References 30 publications

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“…A number of IFN-related genes (including the unknown LB3 (8)) and mx increased 7 days after injection, thereby confirming the observations made in previous studies using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) (Acosta et al, 2005;Robertsen, 2008). Further studies included differential gene expression in kidneys from Japanese flounder injected with the HRV G gene (protective) in comparison with the N gene •, Infection-by-immersion of zebrafish with VHSV and expression on fins (Encinas et al, 2010).…”

Section: Microarrays In the Study Of The Flatfish/hrv/vhsv Modelssupporting

confidence: 88%

“…As established by quantitative RT-qPCR before the advent of microarrays, 4 to 8 days after DNA vaccination by intramuscular injection, gene expression by fish haematopoietic organs showed an increase in interferon-inducible mx (Acosta et al, 2005;Boudinot et al, 1998;McLauchlan et al, 2003;Purcell et al, 2004;Robertsen, 2008;Tafalla et al, 2007), virallyinduced genes (Vig) (Boudinot et al, 1999;Boudinot et al, 2001) and mhc and tcr genes (Takano et al, 2004). 1 cDNA HRV-infected Leukocytes (Aoki et al, 1999) (Nam et al, 2000) European flounder…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Use of Microarray Technology to Improve DNA Vaccines in Fish Aquaculture - The Rhabdoviral Model

Encinas¹,

Gómez-Casado²,

Estepa³

et al. 2012

Health and Environment in Aquaculture

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Section: Microarrays In the Study Of The Flatfish/hrv/vhsv Modelssupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Use of Microarray Technology to Improve DNA Vaccines in Fish Aquaculture - The Rhabdoviral Model

Encinas¹,

Gómez-Casado²,

Estepa³

et al. 2012

Health and Environment in Aquaculture

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“…The crucian carp IRF-7 was induced within 1 h after IFN treatment and 12 h after ultravioletinactivate virus stimulation, suggesting that it is an early response molecule (Zhang et al, 2003). Fish Mx protein is expressed at a relatively late stage 18 h postpoly I: C stimulation (Staeheli et al, 1989), and its increased expression may last a few days (Jensen and Robertsen, 2000;Acosta et al, 2005). So the Mx protein is a relatively stable molecule compared with IRF-1 and IRF-7.…”

Section: Discussionmentioning

confidence: 99%

Gene structure and transcription of IRF-1 and IRF-7 in the mandarin fish Siniperca chuatsi

Sun

Chang

Song

et al. 2007

Veterinary Immunology and Immunopathology

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“…Dis Aquat Org 95: [137][138][139][140][141][142][143] 2011 in rainbow trout by 7 d and lasting up to 21 d (Acosta et al 2005). A DNA vaccine for VHSV conferred shortterm protection of rainbow trout Oncorhynchus mykiss from infection with heterologous rhabdoviruses and the unenveloped nodavirus of turbot (Sommerset et al 2003).…”

Section: Resale or Republication Not Permitted Without Written Consenmentioning

confidence: 99%

Rainbow trout Oncorhynchus mykiss ladderlectin, but not intelectin, binds viral hemorrhagic septicemia virus IVb

Reid¹,

Young²,

Lumsden³

2011

Dis. Aquat. Org.

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The innate immune system of fish is critical for rapid detection and immediate response to infection, as well as to orchestrate the adaptive branch of the immune system. Rainbow trout Oncorhynchus mykiss ladderlectin and intelectin are plasma pattern recognition receptors (PRR) for bacterial and fungal pathogens of rainbow trout, but their role as PRRs for virus is unknown. Viral hemorrhagic septicemia virus (VHSV) IVb is a recently described fish pathogen in the Great Lakes, and rainbow trout can be experimentally infected. Using an indirect enzyme-linked immunosorbent assay, rainbow trout plasma ladderlectin significantly (p < 0.05) bound purified VHSV while int electin did not. In addition, plasma ladderlectin but not intelectin was eluted from a VHSV-conjugated Toyopearl column using EDTA. Protein identification was confirmed with polyclonal antiserum used with slot immunoblot, 1-dimensional sodium dodecyl sulphate polyacrylamide electrophoresis, and Western immunoblot.KEY WORDS: Oncorhychus mykiss · Pattern recognition receptor · Viral hemorrhagic septicemia virus · Innate immunity · Plasma proteins · Binding · Ladderlectin · Intelectin Resale or republication not permitted without written consent of the publisherDis Aquat Org 95: [137][138][139][140][141][142][143] 2011 in rainbow trout by 7 d and lasting up to 21 d (Acosta et al. 2005). A DNA vaccine for VHSV conferred shortterm protection of rainbow trout Oncorhynchus mykiss from infection with heterologous rhabdoviruses and the unenveloped nodavirus of turbot (Sommerset et al. 2003). Resistance was hypothesized to be due to the initial inflammatory response that is likely to produce innate antiviral proteins such as interferon and Mx (Sommerset et al. 2003). Mx proteins are one of the major families of interferon-stimulated proteins resulting from innate immune recognition of viral infection (Ellis 2001). More recently, VHSV has been shown to stimulate multiple components of the innate immune system. In gilthead seabream Sparus aurata, VHSV has been shown to recruit and increase the activity of natural cytotoxic cells (NCC) (Esteban et al. 2008). Rainbow trout TLR9 was upregulated at the site of injection of VHSV glycoprotein G in muscle, suggesting the recruitment of dendritic cells to the site; in the head kidney and spleen there was transcriptional increase of tumor necrosis factor alpha and interleukin-6, which are cytokines known to be upregulated by TLR9 activated by unmethylated CpG DNA (Ortega-Villaizan et al. 2009). In addition, the RIG-I/MAVS pathway of double stranded RNA-stimulated interferon induction has been documented in numerous fish species (Biacchesi et al. 2009), and Mx transcript upregulation has been shown in rainbow trout (Acosta et al. 2005) in response to VHSV infection. In Japanese flounder Paralichthys olivaceus, VHSV infection upregulates the hepatic expression of C3 and complement regulatory proteins (Byon et al. 2006).Lectins are cell-associated or circulating proteins involved in recognition and binding of vir...

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Kinetics of Mx expression in rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar L.) parr in response to VHS–DNA vaccination

Cited by 38 publications

References 30 publications

Use of Microarray Technology to Improve DNA Vaccines in Fish Aquaculture - The Rhabdoviral Model

Use of Microarray Technology to Improve DNA Vaccines in Fish Aquaculture - The Rhabdoviral Model

Gene structure and transcription of IRF-1 and IRF-7 in the mandarin fish Siniperca chuatsi

Rainbow trout Oncorhynchus mykiss ladderlectin, but not intelectin, binds viral hemorrhagic septicemia virus IVb

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