Gene structure and transcription of IRF-1 and IRF-7 in the mandarin fish Siniperca chuatsi

Gene structures and promoter characteristics of interferon regulatory factor 1 (IRF-1), IRF-2 and IRF-7 from snakehead Channa argus

“…At present, fish IRF-1, IRF-2 and IRF-7 genes are shown to be induced by virus, IFN or poly I:C in several species (Collet et al, 2003a,b;Ordas et al, 2006;Sun et al, 2006Sun et al, , 2007Zhang et al, 2003). Our findings also indicated that poly I:C can induce the expression of CaIRF genes in snakehead spleen and liver.…”

“…IRF-2 has been reported in rainbow trout and mandarin fish (Siniperca chuatsi), and homologous to known vertebrate IRF-2 (Collet et al, 2003a,b;Sun et al, 2006). Moreover, IRF-7 has been cloned from goldfish (Carassius auratus) and mandarin fish (Zhang et al, 2003;Sun et al, 2007). The functional studies of fish IRFs showed that they have characteristic properties of mammalian IRFs.…”

“…IRF-1 gene was induced by virus in flounder and poly I:C in trout, turbot, sea bream (Collet et al, 2003a,b;Ordas et al, 2006), and IRF-2 gene was induced by poly I:C in trout and mandarin fish (Collet et al, 2003a,b;Sun et al, 2006). The IRF-7 gene was up-regulated by virus-infection or IFN treatment in goldfish, and induced by poly I:C in mandarin fish (Zhang et al, 2003;Sun et al, 2007). In addition, the putative site for the NF-B transcription factor was found in the promoter of pufferfish IRF-1, rainbow trout IRF-1 and IRF-2 (Richardson et al, 2001;Collet and Secombes, 2002;Collet et al, 2003a,b).…”

Expression analysis of three immune genes Interferon-gamma, Mx and Interferon regulatory factor-1 of Japanese flounder ( Paralichthys olivaceus )

“…Several IRFs have been characterized in model fish and some economically important fish species. For example, IRF-1 had been cloned in Japanese flounder 30 and later in rainbow trout 31 , turbot (Scophthalmus maximus) 32 and mandarin fish (Siniperca chuasti) 33 . Japanese flounder is an important economic marine fish species in the world due to its rapid growth, good taste and high nutritive value.…”

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“…As IRF-1 plays an critical role in immune pathways, it has been cloned successfully in Japanese flounder (Paralichthys olivaceus) (Yabu et al 1998), fugu (Takifugu rubripes) (Richardson, Tay, Goh, Venkatesh, & Brenner, 2001), rainbow trout (Oncorhynchus mykiss) (Collet et al 2003), zebrafish (Danio rerio) (Song et al 2004), turbot (Scophthalmus maximus) (Ordás, Abollo, Costa, Figueras, & Novoa, 2006), sea bream (Sparus aurata) (Ordás et al 2006), mandarin fish (Siniperca chuatsi) (Sun, Chang, Song, Yao, & Nie, 2007), crucian carp (Carassius carassius) (Shi et al 2008), orange-spotted grouper (Epinephelus coioides) (Shi, Zhu, Yin, Zhang, & Gui, 2010), Atlantic cod (Gadus morhua) (Feng et al 2009), large yellow croaker (Pseudosciaena crocea) (Yao, Kong, Huang, & Wang, 2010), and Atlantic salmon (Salmo salar) (Bergan, Kileng, Sun, & Robertsen, 2010). Many studies have shown that IRF-1 plays an important role in fish defense against viral infections (Caipang, Hirono, & Aoki, 2005;Collet et al 2007;Shi et al 2008) and that it is up-regulated by salmon IFN1 and by salmon anemia virus compared with IRF-3 and IRF-7 (Bergan et al 2010).…”