Kinetics of maternally derived antibodies upon intramuscular vaccination against classical swine fever with Suvaxyn ® CSF Marker (CP7_E2alf)

Reduced specificity of Erns antibody ELISAs for samples from piglets with maternally derived antibodies induced by vaccination of sows with classical swine fever marker vaccine CP7_E2alf

“…This strongly suggests that the false‐positive results during the first 4 weeks of age are due to cross‐reactive MDAs. This is in strong contrast to a former study on MDAs induced by vaccination of sows with CP7_E2alf, where the pigtype ELISA showed a high number of false‐positive results (35%), especially in the late phase of the trail where no E2 antibodies were detectable (Schroeder et al., ). In the latter study, the false‐positive results could be eliminated by heat treatment of the serum samples.…”

“…So far, only limited information is available on the influence of MDAs on serology testing to differentiate between infected and vaccinated animals (DIVA principle), which is important if breeding farms are affected by emergency vaccination with the marker vaccine. A previous study reported that approximately 35% of serum samples taken from piglets with a low‐to‐moderate titre of MDAs against CP7_E2alf showed a false‐positive result in the pigtype ELISA, especially in the late phase of the trail where no E2‐specific MDAs are detectable (Schroeder et al., ). In addition, it was reported that after heat treatment all samples were correctly classified negative in that study.…”

Protection of Piglets with Maternally Derived Antibodies from Sows Inoculated with an Attenuated Live Marker Classical Swine Fever Vaccine (Flc-LOM-BErns)

“…In association with the growth of the piglets, the increased catabolism may also affect the decline of the MDAs. The decay of reactivities seemed to be almost linear with clear negative ELISA results at about 10 weeks post farrowing [12]. Depending on the age of piglets, MDAs may have different effects on the development of antibodies by live attenuated CSFV vaccination.…”

“…Lactogenic transfer of maternally derived antibodies (MDAs) from immunized sows is considered an effective and economic way to provide piglets with passive immunity against virus infection before they acquire active immunity via direct vaccination [5,6]. In piglets that have ingested low quantities of colostrum, vaccination induces a strong antigenic stimulus, which is characterized by a normal humoral immune response and resistance to subsequent virus challenge [7][8][9][10][11][12][13]. A number of studies have been conducted to investigate whether pigs that have passive antibodies obtained from colostrum can acquire active immunity after an attenuated live CSF vaccination [7,9]; unfortunately, limited studies were conducted to test the efficacy of passive immunity provided only by colostral antibodies against virulent virus challenge [11,14].…”

A decade of research into classical swine fever marker vaccine CP7_E2alf (Suvaxyn® CSF Marker): a review of vaccine properties

“…The corresponding “CP7_E2alf” vaccinated sows had a maximum Neutralizing Peroxidase Linked Assay (NPLA) titer of 80 to 1280 ND 50 . Recently, MDA kinetics were also investigated after single vaccination of pregnant sows 21 days before farrowing [ 44 ]. The E2 ELISA reactivities showed an almost linear decrease over 10 weeks after which all piglets were tested negative in the ELISA again.…”