A decade of research into classical swine fever marker vaccine CP7_E2alf (Suvaxyn® CSF Marker): a review of vaccine properties

Blome, Sandra; Wernike, Kerstin; Reimann, Ilona; König, Patricia; Moß, Claudia; Beer, Martin

doi:10.1186/s13567-017-0457-y

Cited by 34 publications

(37 citation statements)

References 51 publications

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“…Occurrence of mutations during cell culture passaging is a helpful mechanism to improve replication of artificially constructed genomes of RNA viruses. In this line, multiple mutations have been previously described in the CP7_E2Alf chimera, including one important amino acid change in the E rns reported to be responsible for efficient replication in porcine cells [ 7 ]. The amino acid changes at position T4I and S130R of the “RaPro” E rns are different to the mutations observed in CP7_E2Alf and another mutation known to be important for acquired heparan sulfate binding during cell culture adaptation [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

“…It harbours the E2 coding region of CSFV in the context of the bovine viral diarrhea virus (BVDV) strain CP7 [ 6 ]. This chimeric MLV confers good protection and was characterized in detail [ 7 ]. However, due to close antigenic relationship of CSFV and BVDV, cross-reactive antibodies may result in false-positive reactions and failure in DIVA discrimination [ 8–10 ].…”

Section: Introductionmentioning

confidence: 99%

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Genetically distinct pestiviruses pave the way to improved classical swine fever marker vaccine candidates based on the chimeric pestivirus concept

Postel

Becher

2020

Emerging Microbes & Infections

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Classical swine fever (CSF) is one of the most important viral diseases of pigs. In many countries, the use of vaccines is restricted due to limitations of subunit vaccines with regard to efficacy and onset of protection as well as failure of live vaccines to d ifferentiate i nfected from v accinated a nimals (DIVA principle). Chimeric pestiviruses based on CSF virus (CSFV) and the related bovine viral diarrhea virus (BVDV) have been licensed as live marker vaccines in Europe and Asia, but cross-reactive antibodies can cause problems in DIVA application due to close antigenic relationship. To develop marker vaccine candidates with improved DIVA properties, three chimeric viruses were generated by replacing E rns of CSFV Alfort-Tübingen with homologue proteins of only distantly related pestiviruses. The chimeric viruses “Ra”, “Pro”, and “RaPro” contained E rns sequences of Norway rat and Pronghorn pestiviruses or a combination of both, respectively. In porcine cells, the “Pro” chimera replicated to high titers, while replication of the “Ra” chimera was limited. The “RaPro” chimera showed an intermediate phenotype. All vaccine candidates were attenuated in a vaccination/ challenge trial in pigs, but to different extents. Inoculation induced moderate to high levels of neutralizing antibodies that protected against infection with a genetically heterologous, highly virulent CSFV. Importantly, serum samples of vaccinated animals did not show any cross-reactivity in a CSFV E rns antibody ELISA. In conclusion, the E rns antigen from distantly related pestiviruses can provide a robust serological negative marker for a new generation of improved CSFV marker vaccines based on the chimeric pestivirus concept.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetically distinct pestiviruses pave the way to improved classical swine fever marker vaccine candidates based on the chimeric pestivirus concept

Postel

Becher

2020

Emerging Microbes & Infections

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“…In 2014, a new live attenuated marker vaccine CP7_E2alf was licensed by the European Medicines Agency. The resulting data from Friedrich- Loeffler-Institut showed that “CP7_E2alf” is a new instrument in the tool-box of CSF control and can be used to revisit emergency vaccination scenarios (79). Although the vaccines are currently sufficient to provide effective immune protection, they are not omnipotent.…”

Section: Evolution Of Variants and Vaccine Protectionmentioning

confidence: 99%

Classical Swine Fever in China-An Update Minireview

Zhou

2019

Front. Vet. Sci.

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Classical swine fever (CSF) remains one of the most economically important viral diseases of domestic pigs and wild boar worldwide. The causative agent is CSF virus, it is highly contagious, with high morbidity and mortality rates; as such, it is an OIE-listed disease. Owing to a nationwide policy of vaccinations of pigs, CSF is well-controlled in China, with large-scale outbreaks rarely seen. Sporadic outbreaks are however still reported every year. In order to cope with future crises and to eradicate CSF, China should strengthen and support biosecurity measures such as the timely reporting of suspected disease, technologies for reliable diagnoses, culling infected herds, and tracing possible contacts, as well as continued vaccination and support of research into drug and genetic therapies. This mini-review summarizes the epidemiology of and control strategies for CSF in China.

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“…Therefore the vaccine was safe for use in pregnant animals, even in immunosuppressed ones. The same authors reported that E2 subunit vaccines were also highly safe, apart from a possible local tissue reaction at the injection site (Blome et al, 2017).…”

Section: Classical Swine Fever Vaccinationmentioning

confidence: 99%

“…In order to enable differentiation between vaccine and field strain, many other vaccines were developed. However, it has been shown that the best candidate is the recombinant CP7_E2alf vaccine (Blome et al, 2017). Blome et al (2006) showed that vaccine based on attenuated C strain could cause no damage in vaccinated animals.…”

Section: Classical Swine Fever Vaccinationmentioning

confidence: 99%

Some adverse events following immunization in veterinary medicine

Milićević¹,

Veljović²,

Maksimović-Zorić³

et al. 2018

AVM

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Vaccines are a very effective tool for the prevention and eradication of infective diseases in both veterinary and human medicine. Although for safety reasons, vaccines undergo very strict controls before being placed on the market, the risk of adverse reactions is not eliminated. According to the World Health Organization (WHO), adverse event following immunization (AEFI) is any untoward medical occurrence that follows immunization and which does not necessarily have a causal relationship with the usage of the vaccine. Adverse reactions may arise as a direct consequence of immunization due to the specificity of the vaccine itself, the quality of the vaccine or the immunization errors. In addition, adverse reactions may also be the result of a coincidental relationship between the effect and immunization. However, biological mechanisms of AEFI are very complex. During the mass vaccination campaigns, when a large number of animals are vaccinated in a short period of time, adverse reactions are expected to be the most frequently reported. In Serbia, livestock is currently being vaccinated against Lumpy Skin Disease, Bluetongue, and Classical swine fever.

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A decade of research into classical swine fever marker vaccine CP7_E2alf (Suvaxyn® CSF Marker): a review of vaccine properties

Cited by 34 publications

References 51 publications

Genetically distinct pestiviruses pave the way to improved classical swine fever marker vaccine candidates based on the chimeric pestivirus concept

Genetically distinct pestiviruses pave the way to improved classical swine fever marker vaccine candidates based on the chimeric pestivirus concept

Classical Swine Fever in China-An Update Minireview

Some adverse events following immunization in veterinary medicine

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