Kinetics of Erythropoietic Cell Proliferation in Normal and Anemic Man A New Approach Using Quantitative 14C-Autoradiography

Dormer, Peter G.

doi:10.1016/s0079-6336(73)80006-3

Cited by 41 publications

(33 citation statements)

References 193 publications

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“…The TS values we obtained were strictly related to those drawn, on duplicate samples, from quantitative 14C-TdR cytoautoradiography (Riccardi et al, 1988), which is an accepted method for calculating TS. From the experimentally obtained LI and TS, a number of temporal kinetic parameters can be calculated (Dormer, 1973;Steel, 1977;Ucci et al, 1985), assuming either a steady state or other more complex models for cell proliferation (Steel, 1977). We calculated the Tpot, i.e.…”

Section: Discussionmentioning

confidence: 99%

Cell kinetics in leukaemia and solid tumours studied with in vivo bromodeoxyuridine and flow cytometry

Riccardi

Danova²,

Dionigi³

et al. 1989

Br J Cancer

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Section: Discussionmentioning

confidence: 99%

Cell kinetics in leukaemia and solid tumours studied with in vivo bromodeoxyuridine and flow cytometry

Riccardi

Danova²,

Dionigi³

et al. 1989

Br J Cancer

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“…M3 metaphases were found in normal bone marrow in up to 18%. The calculated generation time for these cells is a maximum of 16 h. Such short cell cycles have only been reported for myeloblasts (Cronkite and Vincent, 1970;Boll and Fuchs, 1970) and proerythroblasts (Dormer, 1973).…”

Section: Discussionmentioning

confidence: 84%

Sister chromatid differentiation and cell‐cycle‐specific patterns in chronic myelocytic leukemia and normal bone marrow

Becher¹,

Schmidt²

1982

Intl Journal of Cancer

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After in vitro incubation with bromodeoxyuridine (BrdUrd)-containing medium, metaphases of Philadelphia (Ph1)-chromosome positive cells in the blood and/or bone marrow of eight untreated patients with chronic myelocytic leukemia (CML) were analysed by means of sister chromatid differentiation (SCD) and compared to 10 normal bone marrows. The majority of proliferating Ph1-positive cells (range: 46-86%) were unable to complete more than one cell cycle during the culture period of 50 h (MI metaphases). Ph1-positive M2 metaphases which had completed two cell cycles were found in the range of 18-54% and almost no M3 metaphases were detected after passage through three cell cycles (0-1%). The corresponding findings in proliferating normal bone-marrow cells were: MI (13-68%), M2 (30-79%) and M3 (0-18%). These data support the notion that there is a prolonged cell cycle time in CML which can be measured by SCD, demonstrating a proportional shift from M3/M2 to MI metaphases. SCD is suggested for kinetic studies on human malignant cell clones characterized by marker chromosomes.

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“…However, cross-reactivity of these antibodies could be a problem in both IHC methods (Shibui et al, 1989;Hoshino et al, 1986) and FCM (Bakker et al, 1989;Raza, 1987 (Figure 3a). T. estimates have also been derived from quantitative analysis of nuclear uptake of one single radioactive DNA precursor (Dormer, 1973). However, this requires analysis of the whole nucleus, which is only possible in cell smears.…”

Section: Can T Of Explants Be Measured In Vitro?mentioning

confidence: 99%

Cell kinetic analysis of murine squamous cell carcinomas: a comparison of single versus double labelling using flow cytometry and immunohistochemistry

Schultz-Hector¹,

Begg²,

Hofland³

et al. 1993

Br J Cancer

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Summary The study was originally set up to measure accurate cell kinetic parameters in two murine squamous cell carcinomas (scc) for comparison with radiobiological data on proliferation during radiotherapy. The tumours, AT84 and AT478, were both moderately well differentiated aneuploid scc. In the course of the study, several Tumour cell kinetic studies are not only essential in investigating the many factors regulating proliferation but have also been shown to be useful for predicting the response to therapy (Silvestrini et al., 1984;Tubiana et al., 1989;Begg et al., 1992;Begg, 1993). Most cell kinetic studies have been carried out using either radio-labelled thymidine or a thymidine analog, which are incorporated into cells undergoing DNA synthesis. Such labelling of S-phase cells can be carried out in vivo or in vitro. In patients, administration of radiolabelled DNA precursors is precluded because of radiation hazards. Consequently, clinical studies have employed labelling of biopsy material in vitro (Silvestrini et al., 1984;Tubiana et al., 1989; The original goal of these studies was to measure accurately the cell kinetic parameters in two murine tumours for correlations with radiobiological characteristics of tumour proliferation during radiotherapy . The radiobiological data were available for tumours at two sizes, necessitating kinetic measurements at both these sizes. It was planned to use both FCM and IHC methods to ensure an accurate and correct description of the cell kinetics. The study was then also used to attempt to answer the questions posed above in these two well described animal tumour models. It thus provides comparisons of FCM and IHC methods, in vitro with in vivo labelling, single vs double labelling, the magnitude of intratumoural variations in kinetic parameters, in addition to the influence of tumour size. Materials and methodsTumours and tumour growth rate AT84 and 478 are both fairly well differentiated murine squamous cell carcinoma lines, derived from spontaneous tumours of the oral and vaginal mucosa respectively. An early, more slowly growing (AT478/4) and a late, much faster (AT478/25) generation of one tumour line were compared.

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Kinetics of Erythropoietic Cell Proliferation in Normal and Anemic Man A New Approach Using Quantitative 14C-Autoradiography

Cited by 41 publications

References 193 publications

Cell kinetics in leukaemia and solid tumours studied with in vivo bromodeoxyuridine and flow cytometry

Cell kinetics in leukaemia and solid tumours studied with in vivo bromodeoxyuridine and flow cytometry

Sister chromatid differentiation and cell‐cycle‐specific patterns in chronic myelocytic leukemia and normal bone marrow

Cell kinetic analysis of murine squamous cell carcinomas: a comparison of single versus double labelling using flow cytometry and immunohistochemistry

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