Cell kinetics in leukaemia and solid tumours studied with in vivo bromodeoxyuridine and flow cytometry

Abstract

Summary During a 15-month period, we used in vivo bromodeoxyuridine (BUDR)

Thymidylate synthase expression and activity: relation to S-phase parameters and 5-fluorouracil sensitivity

“…Tberefore, flow cytometry analysis could be especially informative in determining the doubling time of umour tissue and would therefore allow a logical choice for either a biochemical modulation thrapy or a conconitant radio-chenmoherapy. In addition, as LI can also be used for radiotapy tratment planning by evaluation of cell labelling by flow cytometiy (Riccardi et al, 1988;Marchal et al, 1997) this parameter should be especially relevant in case of concomitant radio-chemotrapy.…”

Comparison of BrdUrd and [3H]TdR incorporation to estimate cell proliferation, cell loss, and potential doubling time in tumor xenografts

“…This method is faster and simpler than PLM and has the added advantage of being applicable to studies of tumor cell proliferation in the patient. The latter feature is important, as the results of several clinical studies indicate that the cell kinetic properties of a tumor may be of prognostic value for response to therapy (5,7,8,12,13,16,19,22,26).…”

An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo‐ and chlorodeoxyuridine incorporated into DNA

“…A mouse monoclonal anti-BrdUrd antibody from Becton Dickinson used in a 1:2 dilution binds to all halogenated deoxyuridines but, when the cells were extensively washed with Tris buffer with a high salt concentration, almost no binding to CldUrd was observed. An immunofluorescence procedure was developed, based on these primary antibodies, raised in different species (rat and mouse), in combination with highly purified second antibodies: FITC conjugated goat antirat and TexasRed conjugated goat antimouse.Key terms: Immunochemistry, double staining procedure, IdUrd, CldUrd, flow cytometry, cell kineticsThe study of experimental and human tumors has been facilitated by the recent introduction of immunocytochemical procedures for the detection of halogenated deoxyuridines incorporated into cellular DNA (1,2,(4)(5)(6)(7)(8)(9)(10)(11)13,15,17,18). These methods are rapid and easy to perform, and data from a single sample can provide several cell kinetic parameters (cf.…”

“…The study of experimental and human tumors has been facilitated by the recent introduction of immunocytochemical procedures for the detection of halogenated deoxyuridines incorporated into cellular DNA (1,2,(4)(5)(6)(7)(8)(9)(10)(11)13,15,17,18). These methods are rapid and easy to perform, and data from a single sample can provide several cell kinetic parameters (cf.…”