Thymidylate synthase expression and activity: relation to S-phase parameters and 5-fluorouracil sensitivity

Mirjolet, Jean François; Barberi‐Heyob, Muriel; Merlin, Jean‐Louis; Marchal, Sophie; Etienne, Marie‐Christine; Milano, G.; Bey, P.

doi:10.1038/bjc.1998.443

Cited by 41 publications

(32 citation statements)

References 20 publications

(8 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TS protein or gene expression has been reported to be strongly associated with response to 5-FU treatment and patient survival after chemotherapy, 11,12,20,31,32) but the correlation between sensitivity to 5-FU and TS activity has been reported to be relatively poor in panels of human tumor cell lines, 9,10) and the absence of any correlation between TS activity and sensitivity to 5-FU has been documented in several recent reports. 16,18,33) The inconsistent results concerning the association between TS expression and response to 5-FU may in part be attributable to the different measurement procedures. The role of TS in sensitivity to 5-FU may be more accurately demonstrated by real-time RT-PCR than by enzyme activity, because it provides a very broad dynamic range.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Measurement of Thymidylate Synthase and Dihydropyrimidine Dehydrogenase mRNA Level in Gastric Cancer by Real‐time RT‐PCR

Fujiwara¹,

Terashima²,

Irinoda³

et al. 2002

Japanese Journal of Cancer Research

View full text Add to dashboard Cite

1, 2) Thus, it is important to select patients in whom treatment is likely to be effective in order to improve the results of chemotherapy. In vitro anticancer drug sensitivity tests, which we developed with this goal in mind, have already yielded favorable results.3) However, tests that involve cell culturing procedures are complicated by the need for special facilities, and there can be problems with failures attributable to low cell growth.Standard regimens for gastric cancer involve some form of concomitant treatment that includes biomodulated 5-fluorouracil (5-FU) chemotherapy. 4,5) The mechanism of action of 5-FU has been explained in terms of incorporation of fluorouridine 5′-triphosphate (FUTP) into RNA, resulting in altered gene expression, or in terms of inhibition of thymidylate synthase (TS) by the active metabolite 5-fluorouridine monophosphate (FdUMP).6-8) Several reports have indicated that TS expression in the tumors was related to the sensitivity to 5-FU-based chemotherapy both in the laboratory and clinically. [9][10][11][12] On the other hand, dihydropyrimidine dehydrogenase (DPD) which is both an initial and a rate-limiting catabolic enzyme of 5-FU has been reported to play an important role in the pharmacokinetics of 5-FU and was correlated with the antitumor effectiveness of 5-FU in cancer cell lines and tumors. 9,[13][14][15][16][17][18] Recently, both basic and clinical researches have shown a correlation between the antitumor effectiveness of 5-FU and TS or DPD mRNA levels in tumors by using semiquantitative reverse-transcription polymerase chain reaction (RT-PCR). 11,12,16,[19][20][21] One of the major advantages of these methods is that they can be performed with ultralow-volume samples as compared with measurement of the enzyme activities and in vitro chemosensitivity tests.A real-time RT-PCR method based on TaqMan fluorescence methodology requires fewer steps after PCR than conventional PCR methods, simplifying the procedures 5 To whom correspondence should be addressed.

show abstract

Section: Discussionmentioning

confidence: 99%

Quantitative Measurement of Thymidylate Synthase and Dihydropyrimidine Dehydrogenase mRNA Level in Gastric Cancer by Real‐time RT‐PCR

Fujiwara¹,

Terashima²,

Irinoda³

et al. 2002

Japanese Journal of Cancer Research

View full text Add to dashboard Cite

show abstract

“…Because this is the only pathway that provides the essential thymidylate precursor for DNA synthesis, and because TS is highly expressed in oral cancer cells (48) and in patients diagnosed with oral cancer (49), TS represents an important and critical target in oral cancer chemotherapy. 5-FU, the most common drug used to treat oral cancer, arrests different types of cancer cells in the S phase of the cell cycle (42,44).…”

Section: Discussionmentioning

confidence: 99%

Quercetin Induces Necrosis and Apoptosis in SCC-9 Oral Cancer Cells

Haghiac

Walle²

2005

Nutrition and Cancer

View full text Add to dashboard Cite

“…Tumor cells were incubated (+10 µL of drug in 190 µL of culture medium) with a range of equivalent DTX concentrations from 0.5 to 500 nM (100 nM of DTX corresponds to 0.35 µg of TiONts from TGA). After 96 h of incubation, cell viability was evaluated using MTS assay (Promega Corporation, Fitchburg, WI, USA) according to Mirjolet et al 15 …”

Section: In Vitro Evaluation Of Nanohybrid Cytotoxicitymentioning

confidence: 99%

Docetaxel-titanate nanotubes enhance radiosensitivity in an androgen-independent prostate cancer model

Mirjolet¹,

Boudon²,

Loiseau³

et al. 2017

IJN

View full text Add to dashboard Cite

IF 4.320International audienceAround 40% of high-risk prostate cancer patients who undergo radiotherapy (RT) will experience biochemical failure. Chemotherapy, such as docetaxel (DTX), can enhance the efficacy of RT. Multidrug resistance mechanisms often limit drug efficacy by decreasing intracellular concentrations of drugs in tumor cells. It is, therefore, of interest to develop nanocarriers of DTX to maintain the drug inside cancer cells and thus improve treatment efficacy. The purpose of this study was to investigate the use of titanate nanotubes (TiONts) to develop a TiONts-DTX nanocarrier and to evaluate its radiosensitizing in vivo efficacy in a prostate cancer model. In vitro cytotoxic activity of TiONts-DTX was evaluated using an MTS assay. The biodistribution of TiONts-DTX was analyzed in vivo by single-photon emission computed tomography. The benefit of TiONts-DTX associated with RT was evaluated in vivo. Eight groups with seven mice in each were used to evaluate the efficacy of the nanohybrid combined with RT: control with buffer IT injection ± RT, free DXL ± RT, TiONts ± RT and TiONts-DXL ± RT. Mouse behavior, health status and tumor volume were monitored twice a week until the tumor volume reached a maximum of 2,000 mm3. More than 70% of nanohybrids were localized inside the tumor 96 h after administration. Tumor growth was significantly slowed by TiONts-DTX associated with RT, compared with free DTX in the same conditions (P=0.013). These results suggest that TiONts-DTX improved RT efficacy and might enhance local control in high-risk localized prostate cancer

show abstract

Thymidylate synthase expression and activity: relation to S-phase parameters and 5-fluorouracil sensitivity

Cited by 41 publications

References 20 publications

Quantitative Measurement of Thymidylate Synthase and Dihydropyrimidine Dehydrogenase mRNA Level in Gastric Cancer by Real‐time RT‐PCR

Quantitative Measurement of Thymidylate Synthase and Dihydropyrimidine Dehydrogenase mRNA Level in Gastric Cancer by Real‐time RT‐PCR

Quercetin Induces Necrosis and Apoptosis in SCC-9 Oral Cancer Cells

Docetaxel-titanate nanotubes enhance radiosensitivity in an androgen-independent prostate cancer model

Contact Info

Product

Resources

About