Kinetics of circulating antigens in pigs experimentally infected with Taenia solium eggs

Nguekam, A.; Zoli, A; Vondou, L.; Pouedet, S.; Assana, Emmanuel; Dorny, Pierre; Brandt, Jef; Losson, Bertrand; Geerts, S.

doi:10.1016/s0304-4017(02)00391-6

Cited by 57 publications

(60 citation statements)

References 15 publications

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“…According to Deckers et al (2008), these temporary antigen responses could be due to aborted infections of developing cysticerci, which were not able to evade the pig's immune system. This hypothesis is supported by the findings of Nguekam et al (2003) and Deckers et al (2008), who found that antigen can be detected from as soon as two weeks post infection, thus before cysts are presumed to be fully developed. A consequence of this observation is that, under field conditions, a positive antigen test not necessarily indicates that the pig harbours infectious cysticerci, which complicates the interpretation of many epidemiological studies.…”

Section: Ag-elisa Resultssupporting

confidence: 74%

Complexities in using sentinel pigs to study Taenia solium transmission dynamics under field conditions

Devleesschauwer

Aryal

Jayaraman

et al. 2013

Veterinary Parasitology

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Section: Ag-elisa Resultssupporting

confidence: 74%

Complexities in using sentinel pigs to study Taenia solium transmission dynamics under field conditions

Devleesschauwer

Aryal

Jayaraman

et al. 2013

Veterinary Parasitology

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“…The Ag-ELISA only detects living cysticerci (Garcia et al 2000;Erhart et al 2002;Nguekam et al 2003b) and rapidly becomes negative when the cysts die after treatment (Nguekam et al 2003a). The Ab-ELISA, on the other hand, detects antibodies against living, degenerating and dead cysticerci and does not allow differentiation between these different kinds of cysts.…”

Section: Discussionmentioning

confidence: 99%

Combined use of an antigen and antibody detection enzyme‐linked immunosorbent assay for cysticercosis as tools in an epidemiological study of epilepsy in Burundi

Prado-Jean

Kanobana

Druet‐Cabanac

et al. 2007

Tropical Med Int Health

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Summaryobjective To evaluate the benefits of the detection of both circulating antibodies (Ab) and antigens (Ag) for the diagnosis of cysticercosis in people with epilepsy. Neurocysticercosis is a cause of neurological diseases world-wide, especially epilepsy. The clinical symptoms of neurocysticercosis are non-specific and diagnosis is often difficult.methods Serum samples were collected from subjects in a matched case-control study for epilepsy in the Kiremba area, Burundi, between March and April 2001 (epileptic cases ¼ 303; controls without epilepsy ¼ 606). The enzyme-linked immunosorbent assay (ELISA) was used for the detection of antibodies (Ab-ELISA) and circulating Ag (Ag-ELISA).results The Ab-ELISA revealed 58.7% positivity in epilepsy cases and 31.4% in healthy controls; and Ag-ELISA revealed 38.3% positivity in epilepsy cases and 20.0% in controls. The matched odds ratios were 3.6 (95%CI: 2.5-4.9) for Ab-ELISA, and 2.9 (95%CI: 2.1-4.3) for Ag-ELISA.conclusion Both Ag-and Ab-ELISA detected a significantly higher number of seropositives among people with epilepsy than among controls. The risk of epilepsy was high in cases with a positive Ag-ELISA, although less important than in cases with positivity for Ab-ELISA. Dead or degenerating cysticerci appear to be more frequently associated with epilepsy than living cysts. The high number of people with circulating Ag of Taenia solium suggests that the study area is a focus of active transmission of the parasite.

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“…The Ag-ELISA was performed as described by Dorny et al (2002), with minor modiϐications. Serum samples were pre--tre ated with 5% trich loroacetic acid (Dorny et al 2000, Nguekam et al 2003 and incubated for 20 min at room temperature to dissociate immune-complexes. After 5 minutes of centrifugation (10000g), the supernatant was neutralized using the same volume of a 0.6 M sodium carbonate/ bicarbonate buffer (pH 9.6).…”

Section: Methodsmentioning

confidence: 99%

“…Viability control is essential for calculating the efϐiciency of the establishment of infection and must be performed for each experiment (Santamaria et al 2002). Several authors have reported difϐiculties in infecting pigs experimentally (Aluja et al 1996, Verastegui et al 2000, Nguekam et al 2003. According to Nguekam et al (2003), the ingestion of whole proglotids is the most efϐi-cient means of infection, which suggests that there may be a factor in fecal matter and/or proglotids that enhances the infectivity of the eggs.…”

Section: Detection Of Circulating Antigen Using Ag-elisamentioning

confidence: 99%

“…Serodiagnosis of cysticercosis through the detection of antibodies against the parasite has been widely evaluated using target antigens ranging from total T. solium extracts of the metacestode to more selected preparations, such as cyst ϐluid, scolex or extracts from external membranes (Nunes et al 2000, Nguekam et al 2003, Soares et al 2006. Other detection methods include highly puriϐied or fractionated parasite glycoproteins (Tsang et al 1991, Aluja et al 1999 or recombinant and synthetic antigens (Sato et al 2003, Handali et al 2004, which increase the sensitivity and the speciϐicity of the tests.…”

Section: Introductionmentioning

confidence: 99%

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Cysticercosis in experimentally and naturally infected pigs: parasitological and immunological diagnosis

et al. 2012

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Our objective was to evaluate the diagnosis of swine cysticercosis by examining "ante mortem" (inspection of the tongue), "post mortem" (inspection and detailed necropsy) and ELISA for research in serum of antibodies (Ab-ELISA) and antigens (Ag-ELISA). Seven (7) pigs were experimentally infected orally with eggs of Taenia solium and another 10 were naturally infected. In the pigs experimentally infected, inspection of the tongue was negative in all animals, in the routine inspection detailed necropsy and cysticercis were identified in all of them. In pigs with heavy natural infection, inspection of the tongue identified cysticerci in two (20%), while at inspection with necropsy the parasites were identified in large quantities in all animals. In ELISA for antibody search (Ab-ELISA) TS-14 recombinant protein was used, and in search for antigen (Ag-ELISA) a monoclonal antibody against this protein. In animals experimentally infected, blood was collected weekly for 140 days. The Ab-ELISA identified an increase in titers of antibody to cysticerci 21 days after infection, and at the end of the experimental period six animals (86%) were positive to the test. The search for circulating antigens (Ag-ELISA) was positive in two pigs 28 to 91 days after infection. All naturally infected pigs were positive for Ag-ELISA and Ab-ELISA. The search for antibodies and antigens by ELISA in serum from 30 pigs of a local farm and without history of cysticercosis was negative. Thus, the use of TS-14 antigen in ELISA test (Ab-ELISA) can be useful for the diagnosis of cysticercosis in pigs with low infection.

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Kinetics of circulating antigens in pigs experimentally infected with Taenia solium eggs

Cited by 57 publications

References 15 publications

Complexities in using sentinel pigs to study Taenia solium transmission dynamics under field conditions

Complexities in using sentinel pigs to study Taenia solium transmission dynamics under field conditions

Combined use of an antigen and antibody detection enzyme‐linked immunosorbent assay for cysticercosis as tools in an epidemiological study of epilepsy in Burundi

Cysticercosis in experimentally and naturally infected pigs: parasitological and immunological diagnosis

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