Kinetics of an extracellular exo-inulinase production from a 5-flourocytosine resistant mutant of Geotrichum candidum using two-factorial design

Mughal, M. S.; Ali, Sikander; Ashiq, Mohammed; Talish, A.S.

doi:10.1016/j.biortech.2009.01.051

Cited by 19 publications

(21 citation statements)

References 16 publications

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“…and Emericella sp., have been r eported to be phytase (Marlida et al, 2010). Mughal et al (2009) reported 45.65 IU/mL inulinase production from G. candidum.…”

Section: Enzyme Based Screeningmentioning

confidence: 99%

Probiotic attributes of a yeast-like fungus, Geotrichum klebahnii

Syal¹,

Vohra²

2014

Afr. J. Microbiol. Res.

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Geotrichum klebahnii, a filamentous yeast-like fungus, was isolated from a cheese sample. Several in vitro tests were carried out for its probiotic characterization. This isolate showed high survival rate of 100 ± 1.8% at low pH (pH 2) and 100 ± 0.9% at high oxbile concentration (1%) and also grew well at 37°C. It showed an auto-aggregation ability of 100.00 ± 1.5% after 20 h of incubation at 37°C, as well as 36.43 ± 0.70% and 52.13 ± 1.50% cell surface hydrophobicity with xylene and n-hexadecane, respectively. It had inhibitory activity against food-borne pathogens such as Salmonella sp., Vibrio sp. and Staphylococcus aureus. G. klebahnii produced the enzymes phytase and inulinase. It was a producer of vitamin B 12 and exoploysaccharides. It assimilated up to 29.42 ± 2.1% cholesterol after 48 h of incubation at 37°C. The organism did not produce gelatinase and DNase assay, indicating its safety as a probiotic microorganism. This is the first report of the probiotic potential of yeast like fungus, G. klebahnii.

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“…and Emericella sp., have been r eported to be phytase (Marlida et al, 2010). Mughal et al (2009) reported 45.65 IU/mL inulinase production from G. candidum.…”

Section: Enzyme Based Screeningmentioning

confidence: 99%

Probiotic attributes of a yeast-like fungus, Geotrichum klebahnii

Syal¹,

Vohra²

2014

Afr. J. Microbiol. Res.

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“…Entre las levaduras, además de Kluyveromyces marxianus que tienen alto potencial para producir inulinasa con altos rendimientos aceptables comercialmente, se han publicado últimamente que, se han seleccionado y mutado nuevas cepas como Geotrichum candidum (Mughal et al, 2009); la levadura marina Pichia guilliermondii (Yu et al, 2009) y se han clonado genes de inulinasa INU1 de Pichia guilliermondii en Pichia pastoris X-33 alcanzándose rendimientos de: 71,85 U/ml en 48 h de cultivo; 127. 7 U/ml en 120 h y 286.8 U/ml en 120 h de fermentación respectivamente, siendo estos valores los más altos publicados a la fecha, aunque esto involucra mayores tiempos de fermentación.…”

Section: Microorganismos Productores De Inulinasaunclassified

Inulinase production by yeat Kluyveromyces marxianus

Castillo

Chamy

2010

Sci. agropecu

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Recibido 04 agosto 2010; aceptado 22 octubre 2010 ResumenEn este trabajo se han revisado los principales avances alcanzados en los últimos veinte años, en el estudio de la fermentación de cepas de Kluyveromyces marxianus para la producción de la enzima inulinasa a partir de inulina pura y fuentes de inulina y la determinación de las propiedades y características de la enzima. Con el propósito de recopilar y esclarecer el mayor conocimiento de ésta área, la investigación de ésta materia ha sido dividida en tres partes: sustratos fuentes de inulina; producción microbiana de inulinasa y caracterización y propiedades de la inulinasa. Se discute la información existente sobre los microorganismos capaces de producir inulinasa los que han sido publicados con datos de rendimiento y en particular las condiciones de fermentación de cepas de Kluyveromyces marxianus para la producción de inulinasa a partir de varias fuentes vegetales de inulina. Se concluye que si bien es cierto existen cepas de Kluyveromyces marxianus capaces de producir satisfactoriamente inulinasa, la investigación para una fuente vegetal de inulina económicamente interesante todavía está en curso. Palabras clave: Kluyveromyces marxianus, inulinasa, inulina. AbstractIn this work the principal advances have been checked reached in the last twenty years, in the study of the fermentation of strains of Kluyveromyces marxianus for the production of the enzyme inulinase from inulin pure and sources of inulina and the determination of the properties and characteristics of the enzyme. With the aim of gathering and clarifying the greater knowledge of this one area, the investigation of this matter has been divided into three principal parts; substrate sources of inulin; microbial production of inulinase and characterization and properties of the inulinase. There is discussed the existing information about the microorganisms capable of producing inulinase those who have been brought by information of performance and especially the conditions of fermentation of strains of Kluyveromyces marxianus for the production of inulinase from several vegetable sources of inulin. One concludes that though it is true there exist strains of Kluyveromyces marxianus capable of producing satisfactorily inulinase, the investigation for a vegetable source of inulin economically interesting still is in process.

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“…According to Cao et al 67 an increase of 53 % in biomass and an increase of 158 % in lipid yield were obtained with the cultivation of a mutated strain of G. robustum. Mughal et al 65 obtained a lipase activity almost 24 times higher after inducing the mutagenesis of G. candidum. Yan et al 68 expressed G. candidum Y162 lipase genes in Pichia pastoris and obtained 55 U mL -1 of activity, and Pan et al 69 , by expressing Geotrichum sp.…”

Section: Production Of Geotrichum Spp Lipasesmentioning

confidence: 99%

“…The reduction in the quantity of inoculum probably reduced the crowding effect (a typical mycological phenomenon that causes self-inhibition in spore fungus germination under crowded conditions). A similar technique for sampling solid cultures of G. candidum and P. camembertii was applied by Aldarf et al 50 In relation to the bioreactor choice, several studies have been conducted in shaken flasks (Table 1), but other types of bioreactors have also been applied successfully for Geotrichum cultivation; in general, the scale up from shaken flasks to a bioreactor system requires optimized cultivation conditions that can be achieved with the factorial designs methods 49,[51][52][53][54]65 . The air lift bioreactor was successfully applied for lipase production from Geotrichum because the absence of mechanical agitation reduces the damage in the mycelium and provides better conditions for obtaining high lipase activity 52,53,60 .…”

Section: Production Of Geotrichum Spp Lipasesmentioning

confidence: 99%

A Review on Geotrichum Lipases: Production, Purification, Immobilization and Applications

Maldonado

Lopes

Aguiar‐Oliveira

et al. 2017

Chem.Biochem.Eng.Q.

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Lipases are enzymes produced from innumerous microorganisms, plants and animal cells. They catalyze reactions of different lipid sources. The Geotrichum fungi are good producers of lipases with high hydrolytic activity and specificity for unsaturated fatty acids. A great number of studies have reported the importance of lipase from this genus and described important fermentation parameters for the enzyme production, such as nutrients, temperature, pH, inoculum, time of fermentation and others. Furthermore, different strategies have been used to purify and immobilize lipases from Geotrichum and innumerous applications are cited in different processes as polyunsaturated fatty acids enrichment, hydrolysis and esterification of fat and oils, synthesis of aromas, biodiesel, and many others. This review highlights fundamental aspects of the production, purification, characterization, immobilization, and the applications of lipases produced by the genus Geotrichum.

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Kinetics of an extracellular exo-inulinase production from a 5-flourocytosine resistant mutant of Geotrichum candidum using two-factorial design

Cited by 19 publications

References 16 publications

Probiotic attributes of a yeast-like fungus, Geotrichum klebahnii

Probiotic attributes of a yeast-like fungus, Geotrichum klebahnii

Inulinase production by yeat Kluyveromyces marxianus

A Review on Geotrichum Lipases: Production, Purification, Immobilization and Applications

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