Kinetics Analysis of the Inhibitory Effects of Alpha-Glucosidase and Identification of Compounds from Ganoderma lipsiense Mycelium

Costa, Tania Maria; Mayer, Diego A.; Siebert, Diogo Alexandre; Micke, Gustavo Amadeu; Alberton, Michele Debiasi; Tavares, Lorena Benathar Ballod; Oliveira, Débora de

doi:10.1007/s12010-020-03239-4

Cited by 14 publications

(12 citation statements)

References 44 publications

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“…The results indicated that these compounds were bound with the same residues at the entrance area of the active site of alpha-glucosidase, as shown in Table 2 and Figure 5. These findings are in a good agreement with the previous studies, which suggested that the binding site of these compounds is around the active site of this enzyme [9][10][11]. It is well-known that the competitive inhibitor will prohibit the substrate to enter the active site of the enzyme.…”

Section: Molecular Dockingsupporting

confidence: 93%

Alpha-Glucosidase Inhibition and Molecular Docking of Isolated Compounds from Traditional Thai Medicinal Plant, Neuropeltis racemosa Wall.

et al. 2022

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Neuropeltis racemosa Wall. (Convolvulaceae) is wildly distributed in Asia. Its stem is used as the component in traditional Thai recipes for treatments of muscle rigidity, skin disorder, dysentery, and hypoglycemia. However, the chemical constituents and biological activities of N. racemosa have not been reported. From a screening assay, N. racemosa stem crude extract showed the potent effect on alpha-glucosidase inhibition at 2 mg/mL as 96.09%. The bioassay-guiding isolation led to 5 compounds that were identified by spectroscopic techniques as scopoletin (1), syringic acid (2), methyl 3-methyl-2-butenoate (3), N-trans-feruloyltyramine (4), and N-trans- coumaroyltyramine (5). Compounds 1, 4, and 5 exhibited an IC50 of 110.97, 29.87, and 0.92 µg/mL, respectively, while the IC50 of positive standard, acarbose was 272.72 µg/mL. Kinetic study showed that compound 1 performed as the mixed-type inhibition mechanism, whereas compounds 4 and 5 displayed the uncompetitive inhibition mechanism. The docking study provided the molecular understanding of isolated aromatic compounds (1, 2, 4 and 5) to alpha-glucosidase. Hence, this study would be the first report of isolated compounds and their anti-alpha-glucosidase activity with the mechanism of action from N. racemosa. Thus, these active compounds will be further studied to be the lead compounds among natural antidiabetic drugs.

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Section: Molecular Dockingsupporting

confidence: 93%

Alpha-Glucosidase Inhibition and Molecular Docking of Isolated Compounds from Traditional Thai Medicinal Plant, Neuropeltis racemosa Wall.

et al. 2022

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“…Phenolic compounds prevent the enzymatic hydrolysis of carbohydrates by inhibiting α-amylase and α-glucosidase, with a mixed mechanism (competitive and uncompetitive) [ 20 ]. Previous studies using in vitro and in vivo testing [ 21 , 22 , 23 ] demonstrated an antidiabetic activity from the rhizome extracts of C. orchioides and C. latifolia .…”

Section: Discussionmentioning

confidence: 99%

Untargeted Metabolomics Analysis Using FTIR and UHPLC-Q-Orbitrap HRMS of Two Curculigo Species and Evaluation of Their Antioxidant and α-Glucosidase Inhibitory Activities

et al. 2021

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Curculigo orchioides and C. latifolia have been used as traditional medicines such as antidiabetic and anticancer. This study measured the total phenolics and flavonoid contents as well as analyzed the functional groups and chemical compounds using Fourier-transform infrared (FTIR) spectra and UHPLC-Q-Orbitrap-HRMS profiling for the discrimination of plant parts, geographical origin, and compounds that presumably have a significant contribution as antioxidant and α-glucosidase inhibitors on both plants. The total phenolics and flavonoids contents in Curculigo species varied from 142.09 to 452.47 mg gallic acid equivalent (GAE/g) and from 0.82 to 5.44 mg quercetin equivalent (QE/g), respectively. The lowest IC50 for antioxidant and α-glucosidase inhibitory activities is presented by C. latifolia from a higher altitude region. Principal component analysis (PCA) from FTIR and UHPLC-Q-Orbitrap-HRMS data could discriminate the plant parts and geographical origin. Partial least squares (PLS) analysis has identified several functional groups, such as O–H, C–H, C=O, C–C, C–O, and chemical compounds, unknown-185 and unknown-85, that are most likely to contribute to the antioxidant and α-glucosidase inhibitory activities.

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“…Nowadays, HPLC coupled with sensitive DAD, fluorometer (FLD), ESI/MS and electrospray ionisation/quadrupole time‐of‐flight/mass spectrometer (ESI/Q‐TOF/MS) detectors are used for the analysis of phenolics, flavonoids, metabolites and phytochemicals (Okai & Higashi‐Okai, 2006; Costa et al ., 2019; Costa et al ., 2020). Iron and γ‐oryzanol contents were analysed in two lyophilised rice hydrolysates, and the results are shown in Table 1.…”

Section: Resultsmentioning

confidence: 99%

Production, iron analysis and consumer perception of functional Thai Sinlek iron rice (Oryza sativa) drink

Koonyosying

Flemming

Kusirisin³

et al. 2020

Int J of Food Sci Tech

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Production, assay of chemical compositions and bioactivity and consumer perception test for Sinlek iron rice. AAPH•, 2,2‐azo‐bis(2‐amidinopropane) dihydrochloride radical; ABTS•, 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulphonic acid radical; GAE, gallic acid equivalent; HPLC/MS, high‐performance liquid chromatography; HPLC/UV, high‐performance liquid chromatography/ultraviolet; ICP/MS, inductively coupled plasma/mass spectrometry; IR, iron rice (regular formula); IR$, iron rice (concentrate formula); TPC, total phenolic content.

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Kinetics Analysis of the Inhibitory Effects of Alpha-Glucosidase and Identification of Compounds from Ganoderma lipsiense Mycelium

Cited by 14 publications

References 44 publications

Alpha-Glucosidase Inhibition and Molecular Docking of Isolated Compounds from Traditional Thai Medicinal Plant, Neuropeltis racemosa Wall.

Alpha-Glucosidase Inhibition and Molecular Docking of Isolated Compounds from Traditional Thai Medicinal Plant, Neuropeltis racemosa Wall.

Untargeted Metabolomics Analysis Using FTIR and UHPLC-Q-Orbitrap HRMS of Two Curculigo Species and Evaluation of Their Antioxidant and α-Glucosidase Inhibitory Activities

Production, iron analysis and consumer perception of functional Thai Sinlek iron rice (Oryza sativa) drink

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