Kinetic study of Pt-cytostatics reactions by HPLC

Brandšteterová, Eva; Kiss, František; Miertuš, Stanislav; Garaj, J.

doi:10.1007/bf01244284

Cited by 6 publications

(2 citation statements)

References 7 publications

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“…The reaction rate of this consecutive substitution is entropically controlled by the chelate effect of the CBDC leaving group. The rate constant for the hydrolysis of carboplatin is distinctly lower than that of cisplatin (at room temperature: carboplatin, k s 8.14Â10 À8 s À1 [12]; cisplatin, k s 6.32Â10 À5 s À1 [13]) and depends on the temperature as well as on the pH value. In the presence of other nucleophiles, e.g.…”

Section: Introductionmentioning

confidence: 99%

Investigations on the Stability of Carboplatin Infusion Solutions

Gust

Schnurr

1999

Monatshefte fuer Chemie

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The stability of the carboplatin market drug products Ribocarbo 1 and Ribocarbo-L 1 in 0.9% sodium chloride or 5% glucose infusion solution was investigated by HPLC analysis using a Nucleosil-120-5-C18 column, an eluent consisting of methanol and an aqueous solution of H 2 SO 4 (0.001 N with Na 2 SO 4 (0.02 M); 10:90 or 5:95 (v/v)), and UV detection.At room temperature, carboplatin is stable in 0.9% NaCl solution for 1 h only. During the following 168 h, a 10% degradation to cisplatin and the intermediate diamminechloro[O 1 -1-carboxylato-1-carboxycyclobutane]platinum(II) takes place. This reaction is independent of the carboplatin concentration used.Under identical conditions in 5% glucose solution the carboplatin concentrations decrease during 72 h by about 2±3% and by 5±6% after 168 h of storage. At 4 C, a 10 mg/ml solution is stable during the experiment, whereas from a 1 mg/ml carboplatin infusion, 2% of the drug are lost after day 7 and about 3% after day 28. Degradation products were unequivocally identi®ed. The presence of highly toxic carboplatin hydrolysis products, however, could be excluded.

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Section: Introductionmentioning

confidence: 99%

Investigations on the Stability of Carboplatin Infusion Solutions

Gust

Schnurr

1999

Monatshefte fuer Chemie

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“…The cytostatics were preseparated from sera on an octadecyl silica microcolumn and eluted successively with a mixture of CH3OH-H20 (1:1) and methanol [74]. The cytostatics were preseparated from sera on an octadecyl silica microcolumn and eluted successively with a mixture of CH3OH-H20 (1:1) and methanol [74].…”

Section: Hplc Of Platinum Anti-cancer Drugs Their Derivatives and Spmentioning

confidence: 99%

A survey of the potential of the high performance liquid chromatography and capillary zone electrophoresis for the determination of platinum and platinum group metals

Sommer

Vlašánková

2000

Chromatographia

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Various platinum group metals (PGMs) can be separated in the form of complexes with monodentate ligands, ion associates or chelates by high performance liquid chromatography (HPLC) or capillary zone electrophoresis (CZE). Ion associates of anionic monodentate PGM complexes with low and high-molecular-mass ammonium cations give satisfactory results in ion interaction HPLC on reversed phase silica in the presence of CH3CN or CH30H modifiers. PGM chelates often show considerable shortcomings due to kinetic hindrance during their formation and must be formed prior to HPLC application. Capillary zone electrophoresis (CZE) brings some new aspects to the separation of PGMs in addition to increasing separation speed and miniaturization of the system. Both methods can be successfully used for the treatment of platinum anti-cancer drugs, their organic impurities, hydrolytic products or metabolites in body fluids. Representative examples are discussed in this paper.

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Adsorption of the Pt^II Anticancer Drug Carboplatin by Mesoporous Silica

Pasqua

Wallner

Kerwood

et al. 2009

Chemistry & Biodiversity

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MCM-41, a mesoporous silica nanomaterial with a high surface area for adsorption of small molecules, is a potential new type of delivery vehicle for therapeutic and diagnostic agents. In this report, we show that MCM-41 adsorbs the front-line anticancer drug carboplatin, [Pt(CBDCA-O,O')(NH3)2] (CBDCA=cyclobutane-1,1-dicarboxylate; 1), which is used to treat ovarian, lung, and other types of cancer. UV/Visible difference absorption spectroscopy shows that MCM-41 adsorbs 1.8+/-0.2% of its own weight of carboplatin after a 24 h exposure to 26.9 mM drug in H2O. The pseudo-first-order rate constant for adsorption of carboplatin by MCM-41, measured using [1H,15N] heteronuclear single quantum coherence (HSQC) NMR, and 15N-labeled carboplatin is k(1)=2.92+/-2.17 x 10(-6) s(-1) at ca. 25 degrees.

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Kinetic study of Pt-cytostatics reactions by HPLC

Cited by 6 publications

References 7 publications

Investigations on the Stability of Carboplatin Infusion Solutions

Investigations on the Stability of Carboplatin Infusion Solutions

A survey of the potential of the high performance liquid chromatography and capillary zone electrophoresis for the determination of platinum and platinum group metals

Adsorption of the Pt^II Anticancer Drug Carboplatin by Mesoporous Silica

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Kinetic study of Pt-cytostatics reactions by HPLC

Cited by 6 publications

References 7 publications

Investigations on the Stability of Carboplatin Infusion Solutions

Investigations on the Stability of Carboplatin Infusion Solutions

A survey of the potential of the high performance liquid chromatography and capillary zone electrophoresis for the determination of platinum and platinum group metals

Adsorption of the PtII Anticancer Drug Carboplatin by Mesoporous Silica

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Adsorption of the Pt^II Anticancer Drug Carboplatin by Mesoporous Silica