Kinetic studies and medium improvement of growth and vitamin B12 production by Acetobacterium sp. in batch culture

Bainotti, A. E.; Setogaichi, Makoto; Nishio, Naomichi

doi:10.1016/0922-338x(96)80585-x

Cited by 11 publications

(6 citation statements)

References 18 publications

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“…However, the mechanism of the stimulatory effect is still largely unclear and possibly different for different microorganisms. It was reported that yeast extract is more effective than a cocktail of vitamins in enhancing the growth rate of acetogenic bacteria growing on methanol (Bainotti et al, 1996). Yeast extract has also been mentioned to form complexes with metals (Farrell et al, 1993;Ramamoorthy and Kushner 1975) but this has not been experimentally measured in sufficient detail yet.…”

Section: Introductionmentioning

confidence: 94%

“…The stimulatory effect of yeast extract on the growth of methylotrophic (Hutten et al, 1980;Mah et al, 1978) and acetogenic (Bainotti et al, 1996;Bomar et al, 1991;Savage and Drake, 1986) microorganisms has been discussed. However, the mechanism of the stimulatory effect is still largely unclear and possibly different for different microorganisms.…”

Section: Introductionmentioning

confidence: 99%

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Effect of yeast extract on speciation and bioavailability of nickel and cobalt in anaerobic bioreactors

Gonzalez-Gil

Jansen

Zandvoort

et al. 2003

Biotech & Bioengineering

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The speciation of metals plays an important role in their bioavailability. In the case of anaerobic reactors for the treatment of wastewaters, the ubiquitous presence of sulfide leads to extensive precipitation of metals like nickel and cobalt, which are essential for the metabolism of the anaerobic microorganisms that carry out the mineralization of the pollutants present in the wastewater. In practice, nickel, cobalt, and iron are added in excessive amounts to full-scale installations. This study is concerned with the complexation of nickel and cobalt with yeast extract and its effect on the biogas production by methanogenic biomass. Adsorptive stripping voltammetry (AdSV) was used to get information about the stability and complexing capacity of the metal-yeast extract complexes formed. Nickel and cobalt form relatively strong organic complexes with yeast extract. The bioavailability of these essential metals in anaerobic batch reactors was dramatically increased by the addition of yeast extract. This is due to the formation of dissolved bioavailable complexes, which favors the dissolution of metals from their sulfides. Trace doses of yeast extract may be effective in keeping additions of essential metals to anaerobic reactors at a minimum.

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Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Effect of yeast extract on speciation and bioavailability of nickel and cobalt in anaerobic bioreactors

Gonzalez-Gil

Jansen

Zandvoort

et al. 2003

Biotech & Bioengineering

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“…All manipulations of medium and cultures were carried out under the O 2 ‐free atmosphere of N 2 gas (100% v/v). Medium preparation, culture techniques and culture conditions were according to the protocol previously reported ( Bainotti et al . 1996 ).…”

Section: Methodsmentioning

confidence: 99%

“…1996 ). The medium was slightly modified (referred to as MF medium) from that previously used for methanol‐CO 2 studies and contained the following materials (l −1 deionized water): methanol, 4 g; sodium formate, 8·5 g unless otherwise specified in the text; NH 4 Cl, 1·0 g; MgSO 4 .7H 2 O, 0·1 g; CoCl 2 .6H 2 O, 0·02 g; 5,6‐dimethylbenzimidazole, 0·02 g; yeast extract, 2·0 g; l ‐cysteine‐HCl.H 2 O, 0·5 g; trace element solution, 80 ml ( Bainotti et al . 1996 ); vitamin solution, 40 ml ( Bainotti et al .…”

Section: Methodsmentioning

confidence: 99%

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Growth kinetics of Acetobacterium sp. on methanol-formate in continuous culture

Bainotti

Nishio

2000

J Appl Microbiol

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A .E . B A IN OT T I A ND N . N IS H IO . 2000.The fermentative metabolism of Acetobacterium sp. grown on methanol-formate in continuous culture is described. The reaction stoichiometry of methanol-formate, including cells, were as follows: CH 3 OH ¦ 1·13HCOOH : 0·87CH 3 COOH ¦ 0·47 cell C. Formate enhanced growth yields by approximately 60% compared with methanol-CO 2 -grown cultures. Comparison of yields on methanol-formate allowed calculation of an energy yield of 1·3 mol ATP per mol acetate formed during homoacetate fermentation. The magnitudes of Y EG , the theoretical maximum yield of Y E , and m, the maintenance coefficient, were determined by growing the organism in methanol-formate and resulted in 16·5 g cell (mol methanol catabolized) −1 and 0·674 mmol methanol catabolized (g cell) −1 h −1 , respectively. It is concluded that formate might replace CO 2 as a source of carboxyl donor.

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Production of Biofuels and Useful Materials by Anaerobic Organisms in Ecosystem of Methane Fermentation

Nakashimada

Nishio

2014

Microbial Production

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Kinetic studies and medium improvement of growth and vitamin B12 production by Acetobacterium sp. in batch culture

Cited by 11 publications

References 18 publications

Effect of yeast extract on speciation and bioavailability of nickel and cobalt in anaerobic bioreactors

Effect of yeast extract on speciation and bioavailability of nickel and cobalt in anaerobic bioreactors

Growth kinetics of Acetobacterium sp. on methanol-formate in continuous culture

Production of Biofuels and Useful Materials by Anaerobic Organisms in Ecosystem of Methane Fermentation

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