Kinetic Optimization of Bacteriorhodopsin Films for Holographic Interferometry

Seitz, Arne; Hampp, Norbert

doi:10.1021/jp993949k

Cited by 29 publications

(14 citation statements)

References 38 publications

(52 reference statements)

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“…The dried PM are stacked on top of each other, focusing in the plane of the matrix, so that a layer with 1 m thickness contains about 200 monolayers [33]. When being illuminated such nanofilms exert the electric potential at 100200 mV, which coincides with the membrane potential of living cells [34]. These factors are of great practical importance for integration of PM into polymeric nanomatrix with keeping photochemical properties.…”

Section: Resultsmentioning

confidence: 99%

Metabolism, Physiology and Biotechnological Applications of Halobacteria

Mosin¹,

Ignatov²

2015

European Journal of Molecular Biotechnology

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Halophiles (lat. "salt-loving") is the taxonomic group of extreme aerobic obligate Gramnegative microorganisms that live in conditions of high salinity -in the seas, salt lakes, saline soils etc. These microorganisms are known to reddish patina on products, preserved with using large quantities of salt (NaCl). Halophiles were isolated for the first time at the beginning of the XX century from the marine flora estuary mud, but their systematic study was started only at the end of the second decade of the XX century. The internal environment of the human body is not suitable for existence of halobacteria, since none of them are known to have pathogenic forms. Halobacteria have great practical potential for using in molecular bioelectronics and bionanotechnology due to their unique ability to convert the energy of sunlight into electrochemical energy of protons H + due to the presence in their cells a special photo transforming retinal containing integral protein -bacteriorhodopsin, the mechanism of action of which has been currently studied in detail. This article describes the characteristics of the metabolism and physiology of halophilic bacteria, as well as a method of biosynthesis and preparation of bacteriorhodopsin from purple membranes of cells of the extreme photoorganotrophic halobacterium Halobacterium halobium.

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Section: Resultsmentioning

confidence: 99%

Metabolism, Physiology and Biotechnological Applications of Halobacteria

Mosin¹,

Ignatov²

2015

European Journal of Molecular Biotechnology

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“…Dried PM are stacked on top of each other, focusing in the plane of the matrix, so that a layer with 1 m thickness contains about 200 monolayers [25]. When illuminated such nanofilms exert the electric potential 100200 mV, which coincides with the membrane potential of living cells [26]. These factors are of great practical importance for integration of PM into polymeric nanomatrix with keeping photochemical properties.…”

Section: Resultsmentioning

confidence: 99%

Studying of Phototransformation of Light Signal by Photoreceptor Pigments - Rhodopsin, Iodopsin and Bacteriorhodopsin

Ignatov¹,

Mosin²

2014

Nanotechnology Research and Practice

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This review article views predominately the structure and function of animal and bacterial photoreceptor pigments (rhodopsin, iodopsin, bacteriorhodopsin) and their aspects of nano-and biotechnological usage. On an example of bacteriorhodopsin is described the method of its isolation from purple membranes of photo-organotrophic halobacterium Halobacterium halobium by cellular autolysis by distilled water, processing of bacterial biomass by ultrasound at 22 KHz, alcohol extraction of low and high-weight molecular impurities, cellular RNA, carotenoids and lipids, the solubilization with 0,5 % (w/v) SDS-Na and subsequent fractionation by methanol and gel filtration chromatography on Sephadex G-200 Column balanced with 0.09 M Tris-borate buffer (pH = 8,35) with 0,1 % (w/v) SDS-Na and 2,5 mM EDTA. Within the framework of the research the mechanism of color perception by the visual analyzer having the ability to analyze certain ranges of the optical spectrum, as colors was studied along with an analysis of the additive mixing of two colors. It was shown that at the mixing of electromagnetic waves with different wavelengths, the visual analyzer perceive them as separate or average wave length corresponding to mix color.Keywords: vision; rhodopsin; iodopsin; bacteriorhodopsin; additive color mixing. IntroductionVision (visual perception) is a process of psycho-physiological processing of the images of surrounding objects, carried out by the visual system, which allows to get an idea of the size, shape and color of surrounding objects, their relative position and distance between them. By means of this animals can receive 90 % of all incoming information to the brain.The function of the visual system is carried out through various interrelated complex structures designated as visual analyzer, consisting of a peripheral part (retina, optic nerve, optic tract) and the central department of combining stem and subcortical centers of the midbrain, as

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“…Dried PM is stacked on top of each other, focusing in the plane of the matrix, so that a layer with 1 m thickness contains about 200 monolayers [25]. When being illuminated the nanofilms exert the electric potential 100200 mV, which coincides with the membrane potential of living cells [26]. These factors are of great practical importance for integration of PM into polymeric nanomatrix with keeping photochemical properties.…”

Section: Bacteriorhodopsin and Its Applicationsmentioning

confidence: 99%

New Nano- and Biotechnological Applications of Bacterial and Animal Photoreceptor Pigments  Bacteriorhodopsin, Rhodopsin and Iodopsin

2016

Nanotechnology Research and Practice

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This paper views predominately the structure and function of animal and bacterial photoreceptor pigments (rhodopsin, iodopsin, bacteriorhodopsin) and new aspects of their nanoand biotechnological usage. On an example of bacteriorhodopsin was described the method of its isolation from purple membranes of photo-organotrophic halobacterium Halobacterium halobium by cellular autolysis by distilled water, processing of bacterial biomass by ultrasound at 22 KHz, alcohol extraction of low and high-weight molecular impurities, cellular RNA, carotenoids and lipids, the solubilization with 0,5 % (w/v) SDS-Na and subsequent fractionation by methanol and gel filtration chromatography on Sephadex G-200 Column balanced with 0.09 M Tris-HCl buffer (pH = 6,76) with 0,1 % (w/v) SDS-Na and 2,5 mM EDTA. Within the framework of the research the mechanism of color perception by the visual analyzer having the ability to analyze certain ranges of the optical spectrum, as colors was studied along with an analysis of the additive mixing of two colors. It was shown that at the mixing of electromagnetic waves with different wavelengths, the visual analyzer perceive them as separate or average wave length corresponding to mix color.

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Kinetic Optimization of Bacteriorhodopsin Films for Holographic Interferometry

Cited by 29 publications

References 38 publications

Metabolism, Physiology and Biotechnological Applications of Halobacteria

Metabolism, Physiology and Biotechnological Applications of Halobacteria

Studying of Phototransformation of Light Signal by Photoreceptor Pigments - Rhodopsin, Iodopsin and Bacteriorhodopsin

New Nano- and Biotechnological Applications of Bacterial and Animal Photoreceptor Pigments  Bacteriorhodopsin, Rhodopsin and Iodopsin

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