Kinetic investigation of penicillin g acylase from a mutant strain of Escherichia coli ATCC 11105 immobilized on oxirane–acrylic beads

Abstract

A B S T R A C T Highly purifiedpenicillin G acylase from a tnutant derivative

Hydrolysis of Penicillin G by Penicillin G Acylase Immobilized on Chitosan Microbeads in Different Reactor Systems

“…It can be also concluded that 6-APA is a noncompetitive and PAA is a competitive inhibitor. This is in agreement with the results previously reported [12,14,15,25,26]. Jianguo et al [25] determined inhibition constants as K iA = 0.009 M and K iB = 0.013 M for PGA immobilized on poly(vinyl acetate-co-divinyl benzene) beads.…”

“…Therefore, the immobilized enzyme has apparently lower affinity for the substrate than the free enzyme. The increase in K M after immobilization of the enzyme was also reported in literature [13,14,16,24,25]. Jianguo et al [25] reported a K M value of 0.033 M, which is almost the same as the K M value obtained in the present study showing the same affinities for the substrate of the immobilized enzymes.…”

“…Jianguo et al [25] reported a K M value of 0.033 M, which is almost the same as the K M value obtained in the present study showing the same affinities for the substrate of the immobilized enzymes. Likewise, some of the previously reported values for K M of the PGA immobilized on different carriers were 4.17 mM [12], 11.36 mM [14], and 3.18 mM [15]. These values are lower than the obtained K M value (32.6 mM) probably due to higher film diffusion resistance to the substrate transport near the chitosan microbeads.…”

“…Bryjak et al [13] characterized the preparation of penicillin acylase immobilized onto acrylic carriers and found that it is more effective than the native one. Erarslan and Guray [14] studied kinetic parameters of PGA immobilized on oxirane-acrylic beads. Erarslan et al [15] determined the kinetic properties of penicillin acylase, derived from a mutant strain of E. coli ATCC 11 105 obtained by chemical mutagenesis.…”

Development of immobilized systems with penicillin acylase from Esherichia coli for production of semisyntetic penicillins

“…Takođe je pokazano i da i slobodnu i imobilisanu PAC inhibira supstrat u višku i oba proizvoda reakcije, pri čemu je 6-APA nekompetitivni, a PAA je kompetitivni inhibitor. Ovi rezultati se slažu sa rezultatima objavljenim u literaturi [29,50,244,246,247]. Za imobilisanu PAC na poli(vinil acetat-ko-diviil benzen) česticama dobijene su vrednosti za K iA i K iB od 0,009 mmol/cm 3 i 0,013 mmol/ cm 3 , redom [246].…”