Kinetic approach for evaluation of total antioxidant activity

Antioxidant activity of blood plasma in Altai aborigines

“…The concentration of Н 2 О 2 in blood plasma was measured using amperometric detection, with planar electrodes and a biological sensor covered with Prussian blue [11]. Screen printed planar elec trodes had the following analytical properties: a sensi tivity of no less than 0.6-0.7 AMcm -2 ; a detection limit of no more than 1 × 10 -7 M; a stability of no less than 1000 measurements without loss of sensitivity; and an operating current of 1 nA to 2 µA.…”

Low-interferences Determination of the Antioxidant Capacity in Fruits Juices Based on Xanthine Oxidase and Mediated Amperometric Measurements in the Reduction Mode

“…The reaction between ascorbic acid and hydrogen peroxide was used to amperometrically evaluate the antioxidant capacity without the production of free radicals. 31 However, this approach has limited applicability because hydrogen peroxide is poorly reactive in the absence of transition metal ions and is ubiquitously present in biological media. 32 Nevertheless, hydrogen peroxide also induces cell death, 33 and a total antioxidant capacity should investigate the protection against both radicals and hydrogen peroxide.…”

“…Another alternative available for the detection of hydrogen peroxide is to operate in reduction mode at low overpotentials using horseradish peroxidase 34 or peroxidase mimicking mediators, such as Prussian blue. 31 The antioxidants may be detected either in batch or flow analysis systems to better control the reactions using microchannel electrodes 35 or the XOD immobilized in a reactor. 36 In this paper, we present an XOD based sensor for evaluating the antioxidant capacity that operates in the reduction mode with low interferences.…”

Age-related changes in blood plasma antioxidant activity in population of the southern Altai

“…Analysis of blood plasma was performed by using an AK-1 portal analyzer "Rusens" LTD (Moscow, Russia) with the 3-electrodes scheme connected to IBM PC. The method of ampere meter detection of H 2 O 2 concentration in blood plasma was used in the work with aid of planar electrodes and a biosensor covered with Prussian blue [2]. The method is based on determination of changes in the H 2 O 2 concentration added to the analyzer sample.…”

“…The plasma peroxidation was performed at the temperature of 37oC by administration of 50 μM of hydrogen peroxide for 8-10 min. The H 2 O 2 level in the plasma was determined on the basis of parameters of calibration graph reproduced on the IBM PC monitor as a result of quant absorption with the Prussian blue covering biosensor of the hydrogen peroxide molecules not inhibited by blood plasma antioxidants [2]. The statistical processing of data was performed by using the software package Statistica 6.0 for Windows.…”