Low-interferences Determination of the Antioxidant Capacity in Fruits Juices Based on Xanthine Oxidase and Mediated Amperometric Measurements in the Reduction Mode

Bucur, Madalina-Petruta; Radulescu, Maria-Cristina; Bucur, Bogdan; Radu, Gabriel Lucian

doi:10.2116/analsci.32.135

Cited by 3 publications

(2 citation statements)

References 38 publications

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“…There are numerous electrodes modified with various electrochemical mediators reported for GSH detection. 23 Prussian blue is a mediator suitable for food analysis 24 that was reported to have a good sensitivity for the detection of GSH. 25 Thorough electrochemical studies have demonstrate that Prussian blue is an effective mediator for several thiolic compounds such as cysteamine or thiocholine, but ineffective for GSH.…”

Section: Resultsmentioning

confidence: 99%

Simple, selective and fast detection of acrylamide based on glutathione S-transferase

Bucur

Radu

2018

RSC Adv.

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Acrylamide (AA) is a toxic compound formed in thermally prepared foods by Maillard reaction. Besides foods, AA may be found in cosmetic products as an impurity of the widely-used non-toxic polyacrylamide. We present a novel, fast and selective detection method based on the amperometric monitoring of the coupling reaction between reduced glutathione (GSH) and AA catalyzed by glutathione S-transferase (GST) to produce an electrochemically inactive compound. We have used electrodes modified with cobalt-phthalocyanine to monitor the decrease of GHS concentration at +300 mV. Our system is simple, does not require supplementary substrates such as 1-chloro-2,4-dinitrobenzene (CDNB) nor have disadvantageous competitive kinetics characteristic to inhibition like signals. Using the optimum concentration of 100 mM GSH we have obtained a linear calibration graph from 7 to 50 mM AA and a limit of detection of 5 mM AA. The method is not affected by interfering compounds usually found in foods and was applied for real sample analysis.

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Section: Resultsmentioning

confidence: 99%

Simple, selective and fast detection of acrylamide based on glutathione S-transferase

Bucur

Radu

2018

RSC Adv.

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“…A chronoamperometric study was performed by Brainina et al [19], where the total antioxidant activity was correlated to the oxidation current of a mediator (potassium ferrocyanide K 4 [Fe(CN) 6 ]) which is formed in the reduction of potassium ferricyanide (K 3 [Fe(CN) 6 ]) in the presence of AOx. Another amperometric xanthine oxidase (XOD)- based sensor was developed by Bucur et al [20]. The quantification of the analytical signal was based on the detection of H 2 O 2 , where xanthine and ascorbic acid were considered antioxidants.…”

Section: Introductionmentioning

confidence: 99%

A Nanoparticle-Based Label-Free Sensor for Screening the Relative Antioxidant Capacity of Hydrosoluble Plant Extracts

David

Şerban

Popa

et al. 2019

Sensors

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One of the most important aspects of the detection of antioxidant compounds is developing a fast screening method. The screening of the overall relative antioxidant capacity (RAC) of several Romanian hydrosoluble plant extracts is the focus of this work. This is important because of the presence of increasing levels of reactive oxygen species (such as H2O2) generates oxidative stress in the human body. The consequences are a large number of medical conditions that can be helped by a larger consumption of plant extracts as food supplements, which do not necessarily contain the specified antioxidant contents. By exploiting the catalytic properties of gold nanoparticles, a specific and sensitive nanoparticle-based label-free electrochemical sensor was developed, where the working parameters were optimized for RAC screening of hydrosoluble plant extracts. First, electrochemical measurements (cyclic voltammetry and amperometry) were used to characterize different nanoparticle-based sensors, revealing the best performance of gold nanoparticle-based sensors, obtaining a RAC of 98% for lavender extracts. The sensing principle is based on the quenching effect of antioxidants for H2O2 amperometric detection, where the decrease in electrical signal suggests an increasing antioxidant capacity. The obtained results were expressed in terms of ascorbic acid and Trolox equivalents in order to be able to correlate our results with classical methods like chemiluminescence and UV-Vis spectrophotometry, where a correlation coefficient of 0.907 was achieved, suggesting a good correlation between electrochemistry and spectrophotometry. Considering these results, the optimized gold nanoparticle-based label-free sensor can be used as a simple, rapid alternative towards classical methods for relative antioxidant capacity detection of hydrosoluble plant extracts.

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Ester flavorants detection in foods with a bienzymatic biosensor based on a stable Prussian blue-copper electrodeposited on carbon paper electrode

Radulescu

Bucur

et al. 2019

Talanta

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Low-interferences Determination of the Antioxidant Capacity in Fruits Juices Based on Xanthine Oxidase and Mediated Amperometric Measurements in the Reduction Mode

Cited by 3 publications

References 38 publications

Simple, selective and fast detection of acrylamide based on glutathione S-transferase

Simple, selective and fast detection of acrylamide based on glutathione S-transferase

A Nanoparticle-Based Label-Free Sensor for Screening the Relative Antioxidant Capacity of Hydrosoluble Plant Extracts

Ester flavorants detection in foods with a bienzymatic biosensor based on a stable Prussian blue-copper electrodeposited on carbon paper electrode

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