Simple, selective and fast detection of acrylamide based on glutathione <i>S</i>-transferase

Bucur, Madalina-Petruta; Bucur, Bogdan; Radu, Gabriel Lucian

doi:10.1039/c8ra02252f

Cited by 13 publications

(11 citation statements)

References 30 publications

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“…Table 4 compares the results of AA determination using methods published so far [17,21,22,[24][25][26] with the current study. It is evident that most of the electrochemical sensors are less sensitive when compared to our newly developed sensor.…”

Section: Discussionmentioning

confidence: 96%

Highly Sensitive Voltammetric Determination of Acrylamide Based on Ibuprofen Capped Mercury Nanoparticles

Sirajuddin

Tagar

Haq

et al. 2021

Sensors

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Highly stable, small-sized and evenly distributed solid mercury nanoparticles capped with ibuprofen (Ibu-HgNPs) were prepared via reduction with hydrazine and capped with ibuprofen as a stabilizing agent. Characterization of Ibu-HgNPs was carried out by UV-Vis spectrophotometry and transmission electron microscopy (TEM). The prepared Ibu-HgNPs were immobilized onto a glassy carbon electrode (GCE) and used for the first time as the sensing element for voltammetric determination of low concentrations of acrylamide (AA) in aqueous solutions. Various parameters such as the type of supporting electrolyte, voltammetric mode, frequency, deposition time, stirring rate and initial potential were optimized to obtain the highest peak current of AA. The sensor delivered the best results in combination with the square wave voltammetry (SWV) mode, with good repeatability (relative standard deviation (RSD) of 25 repetitions was 1.4% for 1000 ppb AA). The study further revealed that Ibu-HgNPs are strongly adhered to GCE and hence do not contaminate the environment even after several runs. The newly developed AA sensor provides linear calibration dependence in the range of 100–1300 ppb with an R2 value of 0.996 and limit of detection (LOD) of 8.5 ppb. Negligible interference was confirmed from several organic compounds, cations and anions. The developed sensor was successfully applied for AA determination in various types of environmental real water samples to prove its practical usefulness and applicability.

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Section: Discussionmentioning

confidence: 96%

Highly Sensitive Voltammetric Determination of Acrylamide Based on Ibuprofen Capped Mercury Nanoparticles

Sirajuddin

Tagar

Haq

et al. 2021

Sensors

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“…A linear calibration graph from 7 to 50 µM acrylamide and a limit of detection of 5 µM acrylamide were achieved. The method was used for analysis of thermally prepared foods such as potato chips and bread [ 33 ].…”

Section: The Innovative Use Of Enzyme Kinetic Particularities To Improve the Selectivitymentioning

confidence: 99%

Addressing the Selectivity of Enzyme Biosensors: Solutions and Perspectives

Bucur

Purcarea²,

Andreescu

et al. 2021

Sensors

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Enzymatic biosensors enjoy commercial success and are the subject of continued research efforts to widen their range of practical application. For these biosensors to reach their full potential, their selectivity challenges need to be addressed by comprehensive, solid approaches. This review discusses the status of enzymatic biosensors in achieving accurate and selective measurements via direct biocatalytic and inhibition-based detection, with a focus on electrochemical enzyme biosensors. Examples of practical solutions for tackling the activity and selectivity problems and preventing interferences from co-existing electroactive compounds in the samples are provided such as the use of permselective membranes, sentinel sensors and coupled multi-enzyme systems. The effect of activators, inhibitors or enzymatic substrates are also addressed by coupled enzymatic reactions and multi-sensor arrays combined with data interpretation via chemometrics. In addition to these more traditional approaches, the review discusses some ingenious recent approaches, detailing also on possible solutions involving the use of nanomaterials to ensuring the biosensors’ selectivity. Overall, the examples presented illustrate the various tools available when developing enzyme biosensors for new applications and stress the necessity to more comprehensively investigate their selectivity and validate the biosensors versus standard analytical methods.

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“…GST is an enzyme that supports detoxification by the enhancement of their polarity through coupling glutathione to Xenobiotic as shown in Fig. 4 [29]. GST activity can be measured spectrophotometrically with reduced glutathione and 1-chloro-2,4-dinitrobenzene [30].…”

Section: Glutathione S-transferases (Gsts)mentioning

confidence: 99%

Chemical Detection of the Toxicity of Nanoparticles of Metals and Metal Oxides

Rashdan¹

2021

Nano BioMed ENG

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The wide applications of nanoparticles increased the demand for their risk assessment, a number of studies on the diverse effects of nanoparticles on various systems have been published. This review provides an overview of the mechanisms of cellular uptake of nanoparticles (NPs) and the advanced toxicological studies of the nanoparticles of metals and metal oxides on various systems (in-vitro and in-vivo).

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Simple, selective and fast detection of acrylamide based on glutathione S-transferase

Cited by 13 publications

References 30 publications

Highly Sensitive Voltammetric Determination of Acrylamide Based on Ibuprofen Capped Mercury Nanoparticles

Highly Sensitive Voltammetric Determination of Acrylamide Based on Ibuprofen Capped Mercury Nanoparticles

Addressing the Selectivity of Enzyme Biosensors: Solutions and Perspectives

Chemical Detection of the Toxicity of Nanoparticles of Metals and Metal Oxides

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