Kinetic analysis of interactions between bispecific monoclonal antibodies and immobilized antigens using a resonant mirror biosensor

Interaction of daunomycin antibiotic with human serum albumin: Investigation by resonant mirror biosensor technique, fluorescence spectroscopy and molecular modeling methods

“…Following each cycle of analysis, the cuvette was regenerated by washing with 1.0 × 10 −5 mol l −1 NaOH [18], and baseline was reestablished with binding buffer. IAsys FASTfit software, Version 2.03 (Affinity Sensors, Cambridge, UK) was used to fit the experimental data according to the procedures described in the software manual, which provided the values of observed binding rate (k on ) at each DM concentration.…”

Assaying cardiac biomarkers for toxicity testing using biosensing and cardiomyocytes derived from human embryonic stem cells

“…Especially useful alternatives for quantification of proteins in complex biological media could be surface-based immuno-biosensors (Marks et al, 2007). Examples of attractive techniques are the acoustic wave, surface plasmon resonance (SPR), and total internal refection fluorescence methods (Liedberg et al, 1983;Kunz et al, 1996;Spangler et al, 2001;Dmitriev et al, 2003).…”

Effects of propyl gallate on interaction between TNF-alpha and sTNFR-I using an affinity biosensor1

“…Changes in refractive index and thickness on the sensing surface occur upon binding (association) of ligate to the immobilized ligand. The IAsys biosensor detects these changes continuously, producing a plot of responses that are measured in arc seconds [24][25][26][27] . Following each interaction, the surface of the cuvette is regenerated to remove bound ligate, and another ligate can then be introduced to the sensing surface.…”