Kineococcus aurantiacus gen. nov., sp. nov., a New Aerobic, Gram-Positive, Motile Coccus with meso-Diaminopimelic Acid and Arabinogalactan in the Cell Wall

Yokota, Akira; Tamura, Tomohiko; Nishii, Tadashi; Hasegawa, Takeshi

doi:10.1099/00207713-43-1-52

Cited by 78 publications

(74 citation statements)

References 11 publications

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“…These characteristics also suggest that strain S2-20 T is not a member of the genus Kineosporia. Members of the genus Kineosporia contain almost equal amounts of LL-and meso-A 2 pm or mainly LL-or meso-A 2 pm as the diaminopimelic acid isomer and galactose, glucose, mannose and ribose as cell-wall sugars and lack anteiso-branched fatty acids (Kudo et al, 1998); members of the genus Kineococcus contain meso-A 2 pm, arabinose and galactose in the cell wall and anteiso-C 15 : 0 as the major fatty acid (Yokota et al, 1993;Phillips et al, 2002;Lee, 2006).…”

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confidence: 99%

“…Desiccation-and radiation-resistant bacteria have been observed in several genera, including Deinococcus, Rubrobacter, Hymenobacter, Methylobacterium, Kocuria and Kineococcus (Rainey et al, 2005; Phillips et al, 2002). The genus Kineococcus was first created by Yokota et al (1993) with the species Kineococcus aurantiacus, based on a single strain isolated from soil. Two more species have been added to the genus in recent years; Kineococcus radiotolerans and Kineococcus marinus were reported in 2002 and 2006, respectively.…”

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confidence: 99%

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Kineococcus xinjiangensis sp. nov., isolated from desert sand

Liu¹,

Peng²,

Wang³

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A brown-orange-pigmented, non-spore-forming, coccus-shaped actinomycete, designated S2-20 T , was isolated from desert sand from Xinjiang Province in China. The isolate stains Grampositive, is motile and produces a brownish diffusible pigment. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S2-20 T was phylogenetically affiliated to the genus Kineococcus, and the sequence similarity to the type strains of Kineococcus species was less than 96 %, making it clear that strain S2-20 T represents a species that is separate from recognized Kineococcus species. Its major fatty acid was anteiso-C 15 : 0 . The major menaquinone was MK-9(H 2 ). Whole-cell hydrolysates of strain S2-20 T contained meso-diaminopimelic acid, arabinose and galactose. The DNA G+C content was 77.8 mol%. On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain S2-20 T should be classified within a novel species of the genus Kineococcus, for which the name Kineococcus xinjiangensis sp. nov. is proposed. The type strain is S2-20 T (5CCTCC AB 207179 T 5KCTC 19474 T ).Various bacterial species have the capacity to survive under conditions that are commonly considered extreme, such as cold, hot, saline and dry environments. Because of our interest in radiation-resistant bacteria and the correlation that has been made between desiccation and radiation resistance (Mattimore & Battista, 1996), we have studied bacteria that survive in the desert. Desiccation-and radiation-resistant bacteria have been observed in several genera, including Deinococcus, Rubrobacter, Hymenobacter, Methylobacterium, Kocuria and Kineococcus (Rainey et al., 2005; Phillips et al., 2002). The genus Kineococcus was first created by Yokota et al. (1993) with the species Kineococcus aurantiacus, based on a single strain isolated from soil. Two more species have been added to the genus in recent years; Kineococcus radiotolerans and Kineococcus marinus were reported in 2002 and 2006, respectively. In the present study, a strain with diffusible pigment production was characterized as a novel member of the genus Kineococcus.Strain S2-20 T was isolated from a sand sample from a desert in Xinjiang Province using tenfold-diluted tryptic soy broth (TSB/10; Difco) agar. Colonies were brownorange, circular, convex with entire edges and produced a brownish diffusible pigment but not clusters after 7 days of incubation on TSB/10 agar at 30 u C. Cell morphology was examined by light microscopy (Olympus) and by electron microscopy (Hitachi) using methods published previously (Glauert, 1991;Bozzola & Russell, 1999). Motility was observed on motility agar (TSB/10 with 0.5 % agar). The isolate could also grow on tenfold-diluted marine agar 2216 (MA/10; Difco) but not on PTYG medium (Phillips et al., 2002).Genomic DNA for amplification of the 16S rRNA gene was extracted as described by Earl et al. (2002). The 16S rRNA gene was amplified by PCR with bacterial universal primers 27F and 1492R (Lane, 1991) and the PCR products were sequenced by Invi...

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confidence: 99%

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confidence: 99%

Kineococcus xinjiangensis sp. nov., isolated from desert sand

Liu¹,

Peng²,

Wang³

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…Polar lipids were extracted using the procedure described by Minnikin et al (1984) and analysed by LC-MS. Molecular species were identified from retention times and mass spectra of LC-MS. Cellular fatty acids were identified with the Sherlock Microbial Identification System (MIDI) after strains were grown on tryptic soy agar (TSA; Becton Dickinson) for 3 days at 30 u C. Genomic DNA was prepared according to the method of Suzuki et al (1999) and the DNA G+C content was quantified by HPLC analysis using a DNA-GC kit (Yamasa, Chiba, Japan). Cell walls were prepared by the methods described by Schleifer & Kandler (1972) and amino acids in the acid hydrolysates of the cell walls were identified by TLC (Harper & Davis, 1979), and by HPLC as their phenylthiocarbamoyl derivatives (Yokota et al, 1993). DNA-DNA hybridizations were carried out with photobiotin-labelled probes in microplate wells as described by Ezaki et al (1989).…”

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Demequina globuliformis sp. nov., Demequina oxidasica sp. nov. and Demequina aurantiaca sp. nov., actinobacteria isolated from marine environments, and proposal of Demequinaceae fam. nov.

Matsuo

Kasai

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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Demequina globuliformis sp. nov., Demequina oxidasica sp. nov. and Demequina aurantiaca sp. nov., actinobacteria isolated from marine environments, and proposal of Demequinaceae fam. nov. Three Gram-stain-positive actinobacterial strains, designated YM24-125 T , YM05-1041 T and YM12-102 T , were isolated from marine environments. Phylogenetic analysis based on 16S rRNA gene sequences indicated that they belonged to the suborder Micrococcineae and were most closely related to members of the genus Demequina with 95.0-98.4 % sequence similarities. Cells of strains YM05-1041 T and YM12-102 T were rod-shaped, similar to members of the genus Demequina, while cells of strain YM24-125 T were cocci to short ovoid rods. All strains grew in the absence of NaCl, but tolerated up to 15 % NaCl. The major menaquinone was demethylmenaquinone DMK-9 (H 4 ) and the major polar lipids were phosphatidylinositol, diphosphatidylglycerol and phosphatidyglycerol. All strains contained straight-chain, isobranched and anteiso-branched saturated fatty acids. The DNA G+C contents were 65.3-67.3 mol%. The peptidoglycans of strains YM05-1041 T and YM12-102 T were the A4b type with ornithine while that of strain YM24-125 T contained ornithine and lysine as diamino acids. Phylogenetic analyses and differences in physiological and biochemical characteristics indicated that these three isolates represent novel species within the genus Demequina, for which the names Demequina globuliformis sp. nov. (with YM24-125 T 5NBRC 106266 T 5KCTC 19747 T 5MBIC 08349 T as the type strain), Demequina oxidasica sp. nov. (with YM05-1041 T 5NBRC 106264 T 5KCTC 19746 T 5MBIC 08346 T as the type strain) and Demequina aurantiaca sp. nov. (with YM12-102 T 5NBRC 106265 T 5KCTC 19745 T 5MBIC 08347 T as the type strain) are proposed. Based on differences in the quinone system, the presence of demethylmenaquinone DMK-9(H 4 ) and the pattern of 16S rRNA signatures, the three novel strains and current members of the genus Demequina represent a unique family within the suborder Micrococcineae. Therefore, the family Demequinaceae fam. nov. is also proposed.The genus Demequina was proposed by Yi et al. (2007) and, at the time of writing, consists of three species: Demequina aestuarii JCM 12123 T , isolated from a tidal flat sediment (Yi et al., 2007), Demequina lutea DSM 19970 T , isolated from a permafrost soil (Finster et al., 2009) and Demequina salsinemoris DSM 22060 T , isolated from mangrove soil (Matsumoto et al., 2010). The genus Demequina is characterized by the presence of demethylmenaquinone in the quinone system and belongs to the family Cellulomonadaceae within the suborder Micrococcineae, order Actinomycetales of the subclass Actinobacteridae (Zhi et al., 2009 The three strains grew well on Luria Bertani (LB) agar (Difco), LB agar supplemented with 2 % (w/v) NaCl (final concentration) and on MA 2216 medium (Difco). The strains were maintained on LB agar supplemented with 2 % NaCl at 30 u C, which was also used for all phenotypic assays unless otherwise stated.M...

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“…At the time of writing, the family Kineosporiaceae included the genera Angustibacter (Tamura et al, 2010), Kineococcus (Yokota et al, 1993), Kineosporia (Pagani & Parenti, 1978) and Quadrisphaera (Maszenan et al, 2005).…”

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confidence: 99%

Pseudokineococcus lusitanus gen. nov., sp. nov., and reclassification of Kineococcus marinus Lee 2006 as Pseudokineococcus marinus comb. nov.

Jurado

Láiz

Ortiz-Martinez

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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A Gram-reaction-positive, motile, coccus-shaped actinobacterium, designated strain T2A-S27T, was isolated from a roof tile in Oporto (Portugal) and studied using a polyphasic approach. The 16S rRNA gene sequence of the novel isolate showed high similarity to that of Kineococcus marinus KST3-3T (97.8 % sequence similarity). Strain T2A-S27T showed lower 16S rRNA gene sequence similarities with other members of the genus Kineococcus and members of the family Kineosporiaceae (<94 %). A phylogenetic tree, based on 16S rRNA gene sequences, showed that strain T2A-S27T formed a coherent clade with the type strain of K. marinus and Quadrisphaera granulorum. The isolate was characterized by the presence of meso-diaminopimelic acid in the cell-wall peptidoglycan, MK-9(H2) as the predominant menaquinone and a polar lipid profile consisting of diphosphatidylglycerol and phosphatidylglycerol. The fatty acid profile was dominated by anteiso-C15 : 0. The DNA G+C content was 76.9 mol%. The low level of DNA–DNA relatedness to K. marinus (46–47 %) and the results of the chemotaxonomic and physiological studies clearly distinguished strain T2A-S27T from recognized species of the genus Kineococcus. On the basis of its phylogenetic position and phenotypic traits, strain T2A-S27T ( = LMG 24148T = CECT 7306T = DSM 23768T) represents a novel species of a new genus in the family Kineosporiaceae, for which the name Pseudokineococcus lusitanus gen. nov., sp. nov. is proposed. The misclassified species K. marinus is transferred to the new genus as Pseudokineococcus marinus comb. nov. The type strain of Pseudokineococcus marinus is KST3-3T ( = KCCM 42250T = NRRL B-24439T).

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Kineococcus aurantiacus gen. nov., sp. nov., a New Aerobic, Gram-Positive, Motile Coccus with meso-Diaminopimelic Acid and Arabinogalactan in the Cell Wall

Cited by 78 publications

References 11 publications

Kineococcus xinjiangensis sp. nov., isolated from desert sand

Kineococcus xinjiangensis sp. nov., isolated from desert sand

Demequina globuliformis sp. nov., Demequina oxidasica sp. nov. and Demequina aurantiaca sp. nov., actinobacteria isolated from marine environments, and proposal of Demequinaceae fam. nov.

Pseudokineococcus lusitanus gen. nov., sp. nov., and reclassification of Kineococcus marinus Lee 2006 as Pseudokineococcus marinus comb. nov.

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