Khellin determination in human serum and urine by high-performance liquid chromatography

El-Yazigi, Adnan; Said, S. A.

doi:10.1002/jps.2600691222

Cited by 10 publications

(4 citation statements)

References 3 publications

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“…[7][8][9] A thorough literature survey has revealed that a limited number of spectrophotometric, fluorimetric, voltametric, electrophoretic, and chromatographic methods have been reported for analysis of khellin. [10][11][12][13][14][15][16] A rapid gas chromatographic assay has been used for the separation and determination of khellin in human serum. [15] A reversed-phase highperformance liquid chromatography (RP-HPLC) method was used for the determination of khellin, visnagin, and visnadin in Ammi visnaga L. fruits and some pharmaceuticals with high and reproducible recoveries.…”

Section: Introductionmentioning

confidence: 99%

QUALITATIVE AND QUANTITATIVE ANALYSIS OF KHELLIN IN AMMI VISNAGA FRUITS AND PHARMACEUTICAL PREPARATIONS USING HPTLC AND HPLC

Alqasoumi

Alam

Anwer

et al. 2013

Journal of Liquid Chromatography & Related Technologies

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& An attempt was made to develop and validate a simple and accurate HPTLC method for the analysis of khellin in Ammi visnaga fruits and some pharmaceutical preparations. HPLC method was developed in order to verify the results of HPTLC analyses. HPTLC method was developed using chloroform-acetone 9:1 (%, v=v) as mobile phase. The analysis was performed on 10 Â 20 cm cm aluminum-backed plates coated with 0.2-mm layers of silica gel 60 F 254 (E-Merck, Germany). Camag TLC Scanner III was used for the UV densitometric scanning. RP-HPLC method was developed using a mobile phase composed of methanol-water 60:40 (%, v=v) and C18-ODS-Hypersil column in isocratic mode. Analyses were done in UV absorbance mode at 248 nm for both. The used HPTLC system was found to give sharp, symmetrical, and well-resolved peak at R f value of 0.29 AE 0.04 and linearity in the range 50-300 ng=spot (r 2 ¼ 0.9939). On the other hand, the peak of khellin was well-resolved at R t 4.73 min and linearity in the range of 100-1000 ng=mL (r 2 ¼ 0.9960) was used in the HPLC method. These results indicated that proposed HPTLC and HPLC methods could be successfully applied for quality control and routine analysis of khellin in commercial formulations.

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Section: Introductionmentioning

confidence: 99%

QUALITATIVE AND QUANTITATIVE ANALYSIS OF KHELLIN IN AMMI VISNAGA FRUITS AND PHARMACEUTICAL PREPARATIONS USING HPTLC AND HPLC

Alqasoumi

Alam

Anwer

et al. 2013

Journal of Liquid Chromatography & Related Technologies

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“…The detection limits of khellin in urine and serum (S/N = 3) were 32.5 and 65.0 ng ml -1 , respectively. The serum khellin levels following the administration of 60 mg ranged from 0.1 to 1.7 µg ml -1 , reported by El-Yazigi and Said, 6 and its detection ranges in urine and serum were 1 -20 and 0.1 -5 µg ml -1 . Therefore, the sensitivity of presented method suffices for the determination of khellin in serum and urine.…”

Section: Calibration and Precisionmentioning

confidence: 68%

“…[6][7][8][9] For human urine and serum, these methods require a pretreatment as a solvent extraction and/or lack of sensitivity. In the present study, it was found that the KHL fluorescence occurred by photoirradiation with hydrogen peroxide and ethanol.…”

mentioning

confidence: 99%

Fluorometric Determination of Khellin in Human Urine and Serum by High-Performance Liquid Chromatography Using Postcolumn Photoirradiation

et al. 2003

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“…[ 9 ] High-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) are the widely accepted analytical techniques for their high accuracy, precision and reproducibility of results whereas, HPTLC has many advantages because of low operating cost and less time consuming. Various analytical methods for determining the khellin content in the plant samples have been reported including thin-layer chromatography (TLC),[ 10 ] gas chromatography,[ 11 ] ultraviolet (UV)-visible spectrophotometric determination,[ 12 ] Fluorometric determination,[ 13 ] capillary electrophoresis,[ 14 ] voltammetry,[ 15 ] HPLC[ 16 17 18 ] and HPTLC. [ 19 ] UV-visible spectrophotometric methods are insufficient in determining an accurate measurement of the khellin due to interference from other constituents in the extracts.…”

mentioning

confidence: 99%

Development and validation of high-performance liquid chromatography and high-performance thin-layer chromatography methods for the quantification of khellin in Ammi visnaga seed

et al. 2015

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Objective:The present study was used to design simple, accurate and sensitive reversed phase-high-performance liquid chromatography RP-HPLC and high-performance thin-layer chromatography (HPTLC) methods for the development of quantification of khellin present in the seeds of Ammi visnaga.Materials and Methods:RP-HPLC analysis was performed on a C18 column with methanol: Water (75: 25, v/v) as a mobile phase. The HPTLC method involved densitometric evaluation of khellin after resolving it on silica gel plate using ethyl acetate: Toluene: Formic acid (5.5:4.0:0.5, v/v/v) as a mobile phase.Results:The developed HPLC and HPTLC methods were validated for precision (interday, intraday and intersystem), robustness and accuracy, limit of detection and limit of quantification. The relationship between the concentration of standard solutions and the peak response was linear in both HPLC and HPTLC methods with the concentration range of 10–80 μg/mL in HPLC and 25–1,000 ng/spot in HPTLC for khellin. The % relative standard deviation values for method precision was found to be 0.63–1.97%, 0.62–2.05% in HPLC and HPTLC for khellin respectively. Accuracy of the method was checked by recovery studies conducted at three different concentration levels and the average percentage recovery was found to be 100.53% in HPLC and 100.08% in HPTLC for khellin.Conclusions:The developed HPLC and HPTLC methods for the quantification of khellin were found simple, precise, specific, sensitive and accurate which can be used for routine analysis and quality control of A. visnaga and several formulations containing it as an ingredient.

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Khellin determination in human serum and urine by high-performance liquid chromatography

Cited by 10 publications

References 3 publications

QUALITATIVE AND QUANTITATIVE ANALYSIS OF KHELLIN IN AMMI VISNAGA FRUITS AND PHARMACEUTICAL PREPARATIONS USING HPTLC AND HPLC

QUALITATIVE AND QUANTITATIVE ANALYSIS OF KHELLIN IN AMMI VISNAGA FRUITS AND PHARMACEUTICAL PREPARATIONS USING HPTLC AND HPLC

Fluorometric Determination of Khellin in Human Urine and Serum by High-Performance Liquid Chromatography Using Postcolumn Photoirradiation

Development and validation of high-performance liquid chromatography and high-performance thin-layer chromatography methods for the quantification of khellin in Ammi visnaga seed

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