Keloid explant culture: a model for keloid fibroblasts isolation and cultivation based on the biological differences of its specific regions

Tucci-Viegas, Vanina Monique; Hochman, Bernardo; França, Jerônimo Pereira de; Ferreira, Lydia Masako

doi:10.1111/j.1742-481x.2010.00698.x

Cited by 29 publications

(32 citation statements)

References 34 publications

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“…The medium in the petri dish was removed and replaced once every three days. 17 The cells were observed until 80% confluent had been achieved, the periodontal tissues were then removed, and the medium was washed with PBS. 18 During the secondary culture stage, 2ml of trypsin-EDTA 0.25% (SM-2003-C Sigma-Aldrich Pte.…”

Section: Methodsmentioning

confidence: 99%

The effects of different 650 nm laser diode irradiation times on the viability and proliferation of human periodontal ligament fibroblast cells

Ismiyatin¹,

Subiyanto²,

Tangdan³

et al. 2019

Dent. J.

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Background: Endo-perio lesions are clinical manifestations of inflammation in the periodontal and pulp tissue. Damage to the periodontal ligament can inhibit its ability to regenerate. Therefore, laser therapy use is expected to improve the prognosis with regard to healing lesions. Unfortunately, the duration of irradiation during laser diode therapy can influence the viability and proliferation of human periodontal ligament fibroblast (hPDLF) cells. Purpose: This study aims to determine the effects of different irradiation exposure times of the 650 nm laser diode of the pulsed mode type on the viability and proliferation of human periodontal ligament fibroblast cells. Methods: This study constituted a laboratory experiment on hPDLF cells using 650 nm laser diode irradiation. Six groups formed the research subjects in this study, namely; two control groups, two radiation groups respectively subjected to irradiation exposure of 15 seconds and 35 seconds duration followed by 24-hour incubation, and two radiation groups exposed to irradiation for 15 and 35 seconds respectively followed by 72-hour incubation period. The viability and proliferation of those cells were subsequently calculated by ELISA reader, while the data was analyzed by means of one-way ANOVA and Tukey tests. Results: The significance value of the viability scores between the 15-second irradiation group and the 35-second irradiation group was less than 0.05, indicating that there was a significant difference between these treatment groups. Similarly, the significance value of proliferation scores between the 15-second irradiation group and the 35-second irradiation group was less than 0.05, again indicating a significant difference between these treatment groups. Conclusion: Irradiation using a 650 nm laser diode 15 seconds and 35 seconds in duration can induce an increase in the viability and proliferation of hPDLF cells.

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Section: Methodsmentioning

confidence: 99%

The effects of different 650 nm laser diode irradiation times on the viability and proliferation of human periodontal ligament fibroblast cells

Ismiyatin¹,

Subiyanto²,

Tangdan³

et al. 2019

Dent. J.

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“…After exeresis of the keloid lesions, samples were obtained from adjacent skin and peripheral keloid (between the excisional margin and the central umbilication) of the surgical specimens using a circular punch of 3 mm diameter, from the epithelialized surface of tissue containing epithelium and connective tissue 18 .…”

Section: Collection Of Keloid Samplesmentioning

confidence: 99%

In vitro effect of 470 nm LED (Light Emitting Diode) in keloid fibroblasts

et al. 2011

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Purpose:To quantify keloid fibroblasts after irradiation with 470nm blue LED, in vitro. Methods: Fibroblasts from keloid and adjacent skin have been obtained from 6 patients. Cells have been cultivated and maintained in DMEM culture medium. In Petri dishes, they were irradiated with energy doses of 6J, 12J and 18J. After 24 h, counting was done by the average of the triplicates for each sample. Results: There were no significant differences in the number of irradiated keloid fibroblasts at the studied doses (p=0.261). In adjacent skin fibroblasts, differences were observed (p=0.025) concerning the doses of 18 J and 6 J (p=0.03). Conclusions: There was a reduction in the number of adjacent skin fibroblasts irradiated with 470nm blue LED at the energy dose of 18 J compared to the ones irradiated at the energy dose of 6 J. There were no changes in keloid fibroblasts counting at any of the doses applied, 24 h after irradiation. Key words: Fibroblasts. Keloid. Phototherapy. Lasers. Skin. Laser Therapy, Low-Level. RESUMOObjetivo: Quantificar fibroblastos de quelóide após irradiação com LED azul de 470nm, in vitro. Métodos: Foram obtidos fibroblastos de quelóide e pele adjacente, de seis pacientes. As células foram cultivadas e mantidas em meio de cultura DMEM. Em placas de Petri, receberam irradiação com doses de energia de 6J, 12J e 18J. Após 24 horas a contagem foi feita pela média da triplicata para cada amostra. Resultados: Não houve diferença na quantidade de fibroblastos de quelóide irradiados nas doses estudadas (p=0,261). Observou-se diferença nos fibroblastos de pele adjacente (p=0,025), com relação às doses de 18 J e 6 J (p=0,03). Conclusões: Houve redução dos fibroblastos de pele adjacente irradiados com LED azul de 470 nm na dose de energia de 18 J em relação à dose de 6 J. Não houve alteração na quantidade de fibroblastos de quelóide nas doses aplicadas após 24 horas da irradiação.

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“…The proliferative effect of neuropeptides on fibroblasts may cause disturbances of wound healing, which may be associated with hyperproliferative skin disorders (e.g., keloids) and mesenchymal disorders (e.g., scleroderma) [36,48,49]. The understanding of the neuropeptidergic action on fibroblasts may provide relevant information to a deeper comprehension of the healing process.…”

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confidence: 99%