Karyotypes in six leukaemia cell lines derived from patients with ALL

Two permanent T-cell leukemia lines designated KH-1 and KH-2 were established from the peripheral blood of a 9-year-old boy with acute lymphoblastic leukemia and a 47-year-old man with adult T-cell leukemia (ATL). No T-cell growth factor was used. KH-1 cells grew as single cells and KH-2 cells formed clusters in suspension culture. Erosette formation, the absence of immunoglobulin determinants and Epstein-Barr-virus-associated nuclear antigen, and the presence of T-cell antigens revealed by monoclonal antibodies were characteristics of these cell lines as in other established T-cell leukemia lines. Chromosome analysis at the beginning revealed mosaic presence of cells with 46, XY, t(8q+; 15q-) and 46, XY which was later completely replaced by the latter karyotype in KH-1, and abnormal karyotype, 47, XY, +3, t (8q-; 10p+) was maintained throughout the period of in vitro passage in KH-2. The donor patient of KH-2 formerly lived in the south-western part of Japan where ATL is considered endemic and numerous type-C virus particles were detected electron microscopically, in KH-2 cell pellets.

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Establishment and cytological characteristics of two in vitro T‐cell lines derived from a child with acute lymphatic leukemia and a man with adult T‐cell leukemia in Japan

Nagasaka

Maeda

Mabuchi

et al. 1982

Intl Journal of Cancer

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Karyotypic characterization of human T‐cell lines immortalized by Herpesvirus saimiri

Troidl

Simmer²,

Fickenscher

et al. 1994

Intl Journal of Cancer

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Herpesvirus saimiri subgroup-C strains transform non-neoplastic human T lymphocytes in vitro to antigen-independent continuous growth. The karyotypes of 8 of these T-cell lines, derived from peripheral blood or bone marrow, are analyzed here. In general, these lines showed a normal diploid karyotype, although one of them had acquired a clonal chromosomal rearrangement after 21 months in culture. Chromosomally aberrant cell clones were found more frequently in cell lines derived from blood lymphocytes of leukemia patients than in cultures from healthy donors. However, the observed aberrations, some of which were defined in detail using chromosome in situ suppression hybridization (CISS) with chromosome-specific recombinant DNA libraries, did not show any relation between the various cell lines nor to the respective types of leukemia.

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Establishment and characterization of a B-cell line from a patient with acute lymphoblastic leukemia

Kottaridis¹,

Perez²,

Kokkinopoulos³

et al. 1985

Leukemia Research

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Karyotypes in six leukaemia cell lines derived from patients with ALL

Cited by 6 publications

References 23 publications

Establishment and cytological characteristics of two in vitro T‐cell lines derived from a child with acute lymphatic leukemia and a man with adult T‐cell leukemia in Japan

Establishment and cytological characteristics of two in vitro T‐cell lines derived from a child with acute lymphatic leukemia and a man with adult T‐cell leukemia in Japan

Karyotypic characterization of human T‐cell lines immortalized by Herpesvirus saimiri

Establishment and characterization of a B-cell line from a patient with acute lymphoblastic leukemia

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