Establishment and characterization of a B-cell line from a patient with acute lymphoblastic leukemia

Biotransformation of the flavonoid tiliroside to 7-methylether tiliroside: bioactivity of this metabolite and of its acetylated derivative

“…Thus for reasons of comparison we examined the metabolic compound 2 and its acetylated derivative 2a for cytotoxic activity on leukemic cells. The following cell lines have been used: CCRF-CEM [11,12], MOLT3 [13], JURKAT [14], HUT78 [15], H9 [16] , (T cells) KM3 [17], NAMALWA [18], DAUDI [19,20], SDK [21], JIYOYE [22] , CCRF-SB [23] (B cell lines), HL60 [24] (promyelocytic cell line), K562 [25] (proerythrocytes), U937 [26] (monocytes). All cell lines were grown as exponentially proliferating suspension cultures in RPMI-1640 medium (Gibco Europe Ltd, Scotland), supplemented with 10% heat inactivated fetal calf serum (Myoclone Gibco), 2 mM L-glutamine (Gibco), and 50 mg/ml gentamycin and incubated at 37°C, in a humidified atmosphere and 5% CO 2 .…”